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Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey.
BMC Complement Altern Med. 2013 Jul 01; 13:151.BC

Abstract

BACKGROUND

Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.

METHODS

Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.

RESULTS

Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.

CONCLUSIONS

Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23815879

Citation

Pimentel, Renah Boanerges de Queiroz, et al. "Antimicrobial Activity and Rutin Identification of Honey Produced By the Stingless Bee Melipona Compressipes Manaosensis and Commercial Honey." BMC Complementary and Alternative Medicine, vol. 13, 2013, p. 151.
Pimentel RB, da Costa CA, Albuquerque PM, et al. Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey. BMC Complement Altern Med. 2013;13:151.
Pimentel, R. B., da Costa, C. A., Albuquerque, P. M., & Junior, S. D. (2013). Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey. BMC Complementary and Alternative Medicine, 13, 151. https://doi.org/10.1186/1472-6882-13-151
Pimentel RB, et al. Antimicrobial Activity and Rutin Identification of Honey Produced By the Stingless Bee Melipona Compressipes Manaosensis and Commercial Honey. BMC Complement Altern Med. 2013 Jul 1;13:151. PubMed PMID: 23815879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey. AU - Pimentel,Renah Boanerges de Queiroz, AU - da Costa,Cristovão Alves, AU - Albuquerque,Patrícia Melchionna, AU - Junior,Sergio Duvoisin, Y1 - 2013/07/01/ PY - 2012/11/03/received PY - 2013/06/24/accepted PY - 2013/7/3/entrez PY - 2013/7/3/pubmed PY - 2014/10/7/medline SP - 151 EP - 151 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 13 N2 - BACKGROUND: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds. METHODS: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm. RESULTS: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee. CONCLUSIONS: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/23815879/Antimicrobial_activity_and_rutin_identification_of_honey_produced_by_the_stingless_bee_Melipona_compressipes_manaosensis_and_commercial_honey_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-151 DB - PRIME DP - Unbound Medicine ER -