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DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus.
Environ Toxicol Pharmacol. 2013 Sep; 36(2):539-547.ET

Abstract

The present study was undertaken to evaluate the genotoxic and oxidative stress modulatory effects of commercial formulation of glyphosate-based herbicide (Roundup(®)) in freshwater fish Channa punctatus. Three sublethal test concentrations of the herbicide viz., SL-I (1/10th of LC50=∼3.25mgL(-1)), SL-II (1/8th of LC50=∼4.07mgL(-1)) and SL-III (1/5th of LC50=∼6.51mgL(-1)) were calculated using 96-LC50 value and the test specimens were exposed to these concentrations. Blood and gill cells of the exposed specimens were sampled on day 1, 7, 14, 21, 28 and 35 to examine the DNA damage using comet assay and to assess the alteration in lipid peroxidation and antioxidant enzymes activities. The highest DNA damage was observed on day 14 at all test concentrations followed by gradual non-linear decline. Induction of oxidative stress in the blood and gill cells were evidenced by increased lipid peroxidation level, while antioxidants namely superoxide dismutase, catalase and glutathione reductase responded in a concentration-dependent manner. The results supported the integrated use of comet and antioxidant assays in determining the toxicity of water pollutants which could be used as part of monitoring programs.

Authors+Show Affiliations

Molecular Biology and Biotechnology Unit Department of Zoology and Environmental Biology, University of Nigeria, Nsukka, Enugu State, Nigeria; Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, UP, India.Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, UP, India.Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, UP, India. Electronic address: ravindra.scientist@gmail.com.Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, UP, India.Molecular Biology and Biotechnology Division, National Bureau of Fish Genetic Resources (Indian Council of Agricultural Research), Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, UP, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23816461

Citation

Nwani, C D., et al. "DNA Damage and Oxidative Stress Modulatory Effects of Glyphosate-based Herbicide in Freshwater Fish, Channa Punctatus." Environmental Toxicology and Pharmacology, vol. 36, no. 2, 2013, pp. 539-547.
Nwani CD, Nagpure NS, Kumar R, et al. DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environ Toxicol Pharmacol. 2013;36(2):539-547.
Nwani, C. D., Nagpure, N. S., Kumar, R., Kushwaha, B., & Lakra, W. S. (2013). DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environmental Toxicology and Pharmacology, 36(2), 539-547. https://doi.org/10.1016/j.etap.2013.06.001
Nwani CD, et al. DNA Damage and Oxidative Stress Modulatory Effects of Glyphosate-based Herbicide in Freshwater Fish, Channa Punctatus. Environ Toxicol Pharmacol. 2013;36(2):539-547. PubMed PMID: 23816461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. AU - Nwani,C D, AU - Nagpure,N S, AU - Kumar,Ravindra, AU - Kushwaha,Basdeo, AU - Lakra,W S, Y1 - 2013/06/07/ PY - 2012/10/07/received PY - 2013/05/30/revised PY - 2013/06/03/accepted PY - 2013/7/3/entrez PY - 2013/7/3/pubmed PY - 2014/3/13/medline KW - Antioxidant enzymes KW - Channa punctatus KW - Comet assay KW - DNA damage KW - Oxidative stress KW - Roundup(®) SP - 539 EP - 547 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 36 IS - 2 N2 - The present study was undertaken to evaluate the genotoxic and oxidative stress modulatory effects of commercial formulation of glyphosate-based herbicide (Roundup(®)) in freshwater fish Channa punctatus. Three sublethal test concentrations of the herbicide viz., SL-I (1/10th of LC50=∼3.25mgL(-1)), SL-II (1/8th of LC50=∼4.07mgL(-1)) and SL-III (1/5th of LC50=∼6.51mgL(-1)) were calculated using 96-LC50 value and the test specimens were exposed to these concentrations. Blood and gill cells of the exposed specimens were sampled on day 1, 7, 14, 21, 28 and 35 to examine the DNA damage using comet assay and to assess the alteration in lipid peroxidation and antioxidant enzymes activities. The highest DNA damage was observed on day 14 at all test concentrations followed by gradual non-linear decline. Induction of oxidative stress in the blood and gill cells were evidenced by increased lipid peroxidation level, while antioxidants namely superoxide dismutase, catalase and glutathione reductase responded in a concentration-dependent manner. The results supported the integrated use of comet and antioxidant assays in determining the toxicity of water pollutants which could be used as part of monitoring programs. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/23816461/DNA_damage_and_oxidative_stress_modulatory_effects_of_glyphosate_based_herbicide_in_freshwater_fish_Channa_punctatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(13)00133-6 DB - PRIME DP - Unbound Medicine ER -