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Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae.
Funct Integr Genomics. 2013 Aug; 13(3):309-22.FI

Abstract

Rice blast is one of the important diseases of rice which can be effectively managed by the deployment of resistance genes. Pi-ta is one of the major blast resistant genes effective against pathogen populations in different parts of India. We analysed allelic variants of Pi-ta from 48 rice lines selected after phenotyping of 529 rice landraces across three eco-geographical blast hot spot regions. Besides, Pi-ta orthologue sequences of 220 rice accessions belonging to wild and cultivated species of rice were also included in the study for a better evo-devo perspective of the diversity present in the gene and the selection pressures acting on this locus. We obtained high nucleotide variations (SNPs and insertion-deletions) in the intronic region. We also identified 64 haplotypes based on nucleotide polymorphism in these alleles. Pi-ta orthologues of Indian landraces were scattered in eight major haplotypes indicating its heterogenous nature. We identified a total of 47 different Pi-ta protein variants on the basis of deduced amino acid residues amongst the orthologues. Five unique and novel Pi-ta variants were identified for the first time in rice landraces exhibiting different reaction types against the Magnaporthe oryzae population. A high value of Pi(non/syn) was observed only in the leucine-rich domain of the alleles cloned from Indian landraces, indicating strong selective forces acting on this region. The detailed molecular analysis of the Pi-ta orthologues provides insights to a high degree of inter- and intraspecific relationships amongst the Oryza species. We identified rice landraces possessing the effective alleles of this resistance gene which can be used in future blast resistance breeding programmes.

Authors+Show Affiliations

National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi 110012, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23818197

Citation

Thakur, S, et al. "Molecular Diversity in Rice Blast Resistance Gene Pi-ta Makes It Highly Effective Against Dynamic Population of Magnaporthe Oryzae." Functional & Integrative Genomics, vol. 13, no. 3, 2013, pp. 309-22.
Thakur S, Gupta YK, Singh PK, et al. Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae. Funct Integr Genomics. 2013;13(3):309-22.
Thakur, S., Gupta, Y. K., Singh, P. K., Rathour, R., Variar, M., Prashanthi, S. K., Singh, A. K., Singh, U. D., Chand, D., Rana, J. C., Singh, N. K., & Sharma, T. R. (2013). Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae. Functional & Integrative Genomics, 13(3), 309-22. https://doi.org/10.1007/s10142-013-0325-4
Thakur S, et al. Molecular Diversity in Rice Blast Resistance Gene Pi-ta Makes It Highly Effective Against Dynamic Population of Magnaporthe Oryzae. Funct Integr Genomics. 2013;13(3):309-22. PubMed PMID: 23818197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae. AU - Thakur,S, AU - Gupta,Y K, AU - Singh,P K, AU - Rathour,R, AU - Variar,M, AU - Prashanthi,S K, AU - Singh,A K, AU - Singh,U D, AU - Chand,D, AU - Rana,J C, AU - Singh,N K, AU - Sharma,T R, Y1 - 2013/07/02/ PY - 2013/02/05/received PY - 2013/05/28/accepted PY - 2013/05/12/revised PY - 2013/7/3/entrez PY - 2013/7/3/pubmed PY - 2013/11/2/medline SP - 309 EP - 22 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 13 IS - 3 N2 - Rice blast is one of the important diseases of rice which can be effectively managed by the deployment of resistance genes. Pi-ta is one of the major blast resistant genes effective against pathogen populations in different parts of India. We analysed allelic variants of Pi-ta from 48 rice lines selected after phenotyping of 529 rice landraces across three eco-geographical blast hot spot regions. Besides, Pi-ta orthologue sequences of 220 rice accessions belonging to wild and cultivated species of rice were also included in the study for a better evo-devo perspective of the diversity present in the gene and the selection pressures acting on this locus. We obtained high nucleotide variations (SNPs and insertion-deletions) in the intronic region. We also identified 64 haplotypes based on nucleotide polymorphism in these alleles. Pi-ta orthologues of Indian landraces were scattered in eight major haplotypes indicating its heterogenous nature. We identified a total of 47 different Pi-ta protein variants on the basis of deduced amino acid residues amongst the orthologues. Five unique and novel Pi-ta variants were identified for the first time in rice landraces exhibiting different reaction types against the Magnaporthe oryzae population. A high value of Pi(non/syn) was observed only in the leucine-rich domain of the alleles cloned from Indian landraces, indicating strong selective forces acting on this region. The detailed molecular analysis of the Pi-ta orthologues provides insights to a high degree of inter- and intraspecific relationships amongst the Oryza species. We identified rice landraces possessing the effective alleles of this resistance gene which can be used in future blast resistance breeding programmes. SN - 1438-7948 UR - https://www.unboundmedicine.com/medline/citation/23818197/Molecular_diversity_in_rice_blast_resistance_gene_Pi_ta_makes_it_highly_effective_against_dynamic_population_of_Magnaporthe_oryzae_ L2 - https://dx.doi.org/10.1007/s10142-013-0325-4 DB - PRIME DP - Unbound Medicine ER -