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Identification of perivascular mesenchymal stromal/stem cells by flow cytometry.
Cytometry A 2013; 83(8):714-20C

Abstract

Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach.

Authors+Show Affiliations

UCLA-Orthopaedic Hospital Department of Orthopaedic Surgery, The Orthopaedic Hospital Research Center, Los Angeles, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

23818229

Citation

Corselli, Mirko, et al. "Identification of Perivascular Mesenchymal Stromal/stem Cells By Flow Cytometry." Cytometry. Part a : the Journal of the International Society for Analytical Cytology, vol. 83, no. 8, 2013, pp. 714-20.
Corselli M, Crisan M, Murray IR, et al. Identification of perivascular mesenchymal stromal/stem cells by flow cytometry. Cytometry A. 2013;83(8):714-20.
Corselli, M., Crisan, M., Murray, I. R., West, C. C., Scholes, J., Codrea, F., ... Péault, B. (2013). Identification of perivascular mesenchymal stromal/stem cells by flow cytometry. Cytometry. Part a : the Journal of the International Society for Analytical Cytology, 83(8), pp. 714-20. doi:10.1002/cyto.a.22313.
Corselli M, et al. Identification of Perivascular Mesenchymal Stromal/stem Cells By Flow Cytometry. Cytometry A. 2013;83(8):714-20. PubMed PMID: 23818229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of perivascular mesenchymal stromal/stem cells by flow cytometry. AU - Corselli,Mirko, AU - Crisan,Mihaela, AU - Murray,Iain R, AU - West,Christopher C, AU - Scholes,Jessica, AU - Codrea,Felicia, AU - Khan,Nusrat, AU - Péault,Bruno, Y1 - 2013/07/01/ PY - 2013/04/03/received PY - 2013/05/07/accepted PY - 2013/7/3/entrez PY - 2013/7/3/pubmed PY - 2014/2/19/medline KW - mesenchymal stem cells KW - pericytes; flow cytometry KW - perivascular cells SP - 714 EP - 20 JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology JO - Cytometry A VL - 83 IS - 8 N2 - Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach. SN - 1552-4930 UR - https://www.unboundmedicine.com/medline/citation/23818229/Identification_of_perivascular_mesenchymal_stromal/stem_cells_by_flow_cytometry_ L2 - https://doi.org/10.1002/cyto.a.22313 DB - PRIME DP - Unbound Medicine ER -