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[Influence of electroacupuncture on p38-mitogen activated protein kinase in substantia nigra cells of rats with Parkinson disease model].

Abstract

OBJECTIVE

To explore the role of inflammatory reaction mediated by p38-mitogen activated protein kinase (p38-MAPK) signal path on prevention and treatment of Parkinson disease (PD) model rats by electroacupuncture (EA).

METHODS

Thirty-two healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and an EA group, eight rats in each one. The PD model was established in the model group and EA group by subcutaneous injection of rotenone in skin-back area (2 mg/kg, dissolved in sunflower oil, 2 mg/mL in density), while the injection of sunflower oil emulsion without rotenone at the same point and quantity as the model group was applied in the sham operation group. The normal group was not given any intervention. The EA treatment (continuous wave, 2 Hz in frequency, 1 mA in intensity, 20 min) was applied at "Fengfu" (GV 16) and "Taichong" (LR 3) in the EA group, once a day for continuously 14 days. No treatment was given in the other groups. The expression of tyrosine hydroxylase (TH), phosphorylated p38-MAPK, cyclooxygenase-2 (COX-2) in the substantia nigra were detected with immunohistochemical method.

RESULTS

There was typical PD ethology change in the model group. Compared with the normal group and sham operation group, the expression of TH positive neuron in the substantia nigra in the model group was significantly decreased, while the expression of phosphorylated p38-MAPK and COX-2 were significantly increased (all P < 0.01). Compared with the model group, the expression of TH positive neuron in the EA group was apparently increased, while the expression of phosphorylated p38-MAPK and COX-2 were significantly decreased (all P < 0.01).

CONCLUSION

The EA therapy could obviously reduce the expression of inflammation mediator COX-2, inhibit the phosphorylation of p38-MAPK, reduce the damage of dopaminergic neurons in the rats with PD, and this effect may be related with the impact of p38-MAPK signal path

Authors+Show Affiliations

,

Orthopedics Department of Hubei University of CM, Wuhan 430065, China.

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Source

MeSH

Animals
Cyclooxygenase 2
Electroacupuncture
Humans
Male
Parkinson Disease
Phosphorylation
Rats
Rats, Sprague-Dawley
Substantia Nigra
Tyrosine 3-Monooxygenase
p38 Mitogen-Activated Protein Kinases

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23819239

Citation

Wang, Shu-Ju, et al. "[Influence of Electroacupuncture On P38-mitogen Activated Protein Kinase in Substantia Nigra Cells of Rats With Parkinson Disease Model]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 33, no. 4, 2013, pp. 329-33.
Wang SJ, Fang JQ, Ma J, et al. [Influence of electroacupuncture on p38-mitogen activated protein kinase in substantia nigra cells of rats with Parkinson disease model]. Zhongguo Zhen Jiu. 2013;33(4):329-33.
Wang, S. J., Fang, J. Q., Ma, J., Wang, Y. C., Zeng, X. L., Zhou, D., & Sun, G. J. (2013). [Influence of electroacupuncture on p38-mitogen activated protein kinase in substantia nigra cells of rats with Parkinson disease model]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 33(4), pp. 329-33.
Wang SJ, et al. [Influence of Electroacupuncture On P38-mitogen Activated Protein Kinase in Substantia Nigra Cells of Rats With Parkinson Disease Model]. Zhongguo Zhen Jiu. 2013;33(4):329-33. PubMed PMID: 23819239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of electroacupuncture on p38-mitogen activated protein kinase in substantia nigra cells of rats with Parkinson disease model]. AU - Wang,Shu-Ju, AU - Fang,Jian-Qiao, AU - Ma,Jun, AU - Wang,Yan-Chun, AU - Zeng,Xiao-Ling, AU - Zhou,Dan, AU - Sun,Guo-Jie, PY - 2013/7/4/entrez PY - 2013/7/4/pubmed PY - 2013/8/14/medline SP - 329 EP - 33 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 33 IS - 4 N2 - OBJECTIVE: To explore the role of inflammatory reaction mediated by p38-mitogen activated protein kinase (p38-MAPK) signal path on prevention and treatment of Parkinson disease (PD) model rats by electroacupuncture (EA). METHODS: Thirty-two healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and an EA group, eight rats in each one. The PD model was established in the model group and EA group by subcutaneous injection of rotenone in skin-back area (2 mg/kg, dissolved in sunflower oil, 2 mg/mL in density), while the injection of sunflower oil emulsion without rotenone at the same point and quantity as the model group was applied in the sham operation group. The normal group was not given any intervention. The EA treatment (continuous wave, 2 Hz in frequency, 1 mA in intensity, 20 min) was applied at "Fengfu" (GV 16) and "Taichong" (LR 3) in the EA group, once a day for continuously 14 days. No treatment was given in the other groups. The expression of tyrosine hydroxylase (TH), phosphorylated p38-MAPK, cyclooxygenase-2 (COX-2) in the substantia nigra were detected with immunohistochemical method. RESULTS: There was typical PD ethology change in the model group. Compared with the normal group and sham operation group, the expression of TH positive neuron in the substantia nigra in the model group was significantly decreased, while the expression of phosphorylated p38-MAPK and COX-2 were significantly increased (all P < 0.01). Compared with the model group, the expression of TH positive neuron in the EA group was apparently increased, while the expression of phosphorylated p38-MAPK and COX-2 were significantly decreased (all P < 0.01). CONCLUSION: The EA therapy could obviously reduce the expression of inflammation mediator COX-2, inhibit the phosphorylation of p38-MAPK, reduce the damage of dopaminergic neurons in the rats with PD, and this effect may be related with the impact of p38-MAPK signal path SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/23819239/ L2 - http://www.diseaseinfosearch.org/result/5603 DB - PRIME DP - Unbound Medicine ER -