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Effect of biphasic electrical current stimulation on IL-1β-stimulated annulus fibrosus cells using in vitro microcurrent generating chamber system.
Spine (Phila Pa 1976) 2013; 38(22):E1368-76S

Abstract

STUDY DESIGN

Human annulus fibrosus (AF) cells were stimulated in vitro with interleukin (IL)-1β and exposed to biphasic electrical currents.

OBJECTIVE

To identify the effect of biphasic electrical currents on the production of the extracellular matrix-modifying enzymes and inflammatory mediators in IL-1β-stimulated AF cells.

SUMMARY OF BACKGROUND DATA

Symptomatic disc degeneration is an important cause of chronic intractable lumbar pain and is associated with macrophage-mediated inflammation in the AF. The inflammatory reaction relationship has not been studied in the AF.

METHODS

Human AF cells were treated with 1 ng/mL IL-1β and cultured in a microcurrent generating chamber system. The levels of matrix metalloproteinase (MMP)-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, IL-6, IL-8, vascular endothelial growth factor (VEGF), insulin-like growth factor, and nitric oxide (NO) were measured. Expression of cyclooxygenase 2 and type I collagen mRNA was analyzed.

RESULTS

Compared with unstimulated cells, IL-1β-stimulated AF cells produced significantly higher levels of MMP-1, MMP-3, IL-6, IL-8, NO, and VEGF, and lower levels of TIMP-1 and TIMP-2. Exposure to a 250-mV/mm field induced time-dependent increases in IL-6, NO, MMP-1, TIMP-1, VEGF, and insulin-like growth factor-1 production. The cells exposed to 500-mV/mm field produced significantly less MMP-1, TIMP-1, IL-6, and VEGF than unexposed cells (MMP-1, 17.2 ± 4.7 ng/mL vs. 27.3 ± 3.9 ng/mL, P< 0.05; TIMP-1, 12.4 ± 3.3 ng/mL vs. 22.3 ± 2.1 ng/mL, P< 0.02; IL-6, 2.5 ± 0.9 ng/mL vs. 6.39 ± 1.90 ng/mL, P< 0.05; and VEGF, 0.1 ± 0.04 ng/mL vs. 0.44 ± 0.15 ng/mL, P< 0.03). NO production was markedly increased at 500 mV/mm (P< 0.0001).

CONCLUSION

We showed that exposure of IL-1β-stimulated AF cells to a 500 mV/mm inhibited MMP-1, IL-6, VEGF, and TIMP-1 production. The results suggest that biphasic electrical current stimulation may have efficacy in diminishing symptomatic disc degeneration.

LEVEL OF EVIDENCE

N/A.

Authors+Show Affiliations

*Department of Neurosurgery, Guro Hospital, College of Medicine, Korea University, Seoul, South Korea †Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul, South Korea ‡Seoul International School, Seoul, Korea §Medical Devices Clinical Trial Center, Guro Hospital, Korea University, Seoul, Korea; and ¶Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23823576

Citation

Kim, Joo Han, et al. "Effect of Biphasic Electrical Current Stimulation On IL-1β-stimulated Annulus Fibrosus Cells Using in Vitro Microcurrent Generating Chamber System." Spine, vol. 38, no. 22, 2013, pp. E1368-76.
Kim JH, Choi H, Suh MJ, et al. Effect of biphasic electrical current stimulation on IL-1β-stimulated annulus fibrosus cells using in vitro microcurrent generating chamber system. Spine. 2013;38(22):E1368-76.
Kim, J. H., Choi, H., Suh, M. J., Shin, J. H., Hwang, M. H., & Lee, H. M. (2013). Effect of biphasic electrical current stimulation on IL-1β-stimulated annulus fibrosus cells using in vitro microcurrent generating chamber system. Spine, 38(22), pp. E1368-76. doi:10.1097/BRS.0b013e3182a211e3.
Kim JH, et al. Effect of Biphasic Electrical Current Stimulation On IL-1β-stimulated Annulus Fibrosus Cells Using in Vitro Microcurrent Generating Chamber System. Spine. 2013 Oct 15;38(22):E1368-76. PubMed PMID: 23823576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of biphasic electrical current stimulation on IL-1β-stimulated annulus fibrosus cells using in vitro microcurrent generating chamber system. AU - Kim,Joo Han, AU - Choi,Hyuk, AU - Suh,Min Ji, AU - Shin,Jae Hee, AU - Hwang,Min Ho, AU - Lee,Heung-Man, PY - 2013/7/5/entrez PY - 2013/7/5/pubmed PY - 2014/5/13/medline SP - E1368 EP - 76 JF - Spine JO - Spine VL - 38 IS - 22 N2 - STUDY DESIGN: Human annulus fibrosus (AF) cells were stimulated in vitro with interleukin (IL)-1β and exposed to biphasic electrical currents. OBJECTIVE: To identify the effect of biphasic electrical currents on the production of the extracellular matrix-modifying enzymes and inflammatory mediators in IL-1β-stimulated AF cells. SUMMARY OF BACKGROUND DATA: Symptomatic disc degeneration is an important cause of chronic intractable lumbar pain and is associated with macrophage-mediated inflammation in the AF. The inflammatory reaction relationship has not been studied in the AF. METHODS: Human AF cells were treated with 1 ng/mL IL-1β and cultured in a microcurrent generating chamber system. The levels of matrix metalloproteinase (MMP)-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, IL-6, IL-8, vascular endothelial growth factor (VEGF), insulin-like growth factor, and nitric oxide (NO) were measured. Expression of cyclooxygenase 2 and type I collagen mRNA was analyzed. RESULTS: Compared with unstimulated cells, IL-1β-stimulated AF cells produced significantly higher levels of MMP-1, MMP-3, IL-6, IL-8, NO, and VEGF, and lower levels of TIMP-1 and TIMP-2. Exposure to a 250-mV/mm field induced time-dependent increases in IL-6, NO, MMP-1, TIMP-1, VEGF, and insulin-like growth factor-1 production. The cells exposed to 500-mV/mm field produced significantly less MMP-1, TIMP-1, IL-6, and VEGF than unexposed cells (MMP-1, 17.2 ± 4.7 ng/mL vs. 27.3 ± 3.9 ng/mL, P< 0.05; TIMP-1, 12.4 ± 3.3 ng/mL vs. 22.3 ± 2.1 ng/mL, P< 0.02; IL-6, 2.5 ± 0.9 ng/mL vs. 6.39 ± 1.90 ng/mL, P< 0.05; and VEGF, 0.1 ± 0.04 ng/mL vs. 0.44 ± 0.15 ng/mL, P< 0.03). NO production was markedly increased at 500 mV/mm (P< 0.0001). CONCLUSION: We showed that exposure of IL-1β-stimulated AF cells to a 500 mV/mm inhibited MMP-1, IL-6, VEGF, and TIMP-1 production. The results suggest that biphasic electrical current stimulation may have efficacy in diminishing symptomatic disc degeneration. LEVEL OF EVIDENCE: N/A. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/23823576/Effect_of_biphasic_electrical_current_stimulation_on_IL_1β_stimulated_annulus_fibrosus_cells_using_in_vitro_microcurrent_generating_chamber_system_ L2 - http://dx.doi.org/10.1097/BRS.0b013e3182a211e3 DB - PRIME DP - Unbound Medicine ER -