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Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats.
Food Chem Toxicol. 2013 Sep; 59:485-91.FC

Abstract

Nymphaea pubescens Willd. is used as ingredient of ethnic diet and folk medicine in South-East Asia. The water (NPW), methanol (NPM) and chloroform (NPC) extracts of N. pubescens flowers were investigated for NO·, O2·[-] and DPPH radical scavenging and iron chelating activities in vitro. NPW was found to be the most potent free radical scavenger (EC50<100 μg/mL) whereas NPC did not show EC50 at 500 μg/mL. Therefore, NPW was selected for further studies on anti-inflammatory and hepatoprotective activities, using standard in vitro and in vivo models. NPW exhibited inhibition of nitrogen radical generation in LPS-activated macrophages (IC50=75.5 μg/mL) through suppression of iNOS protein, with no associated toxicity in the cells. Further, 500 mg/kg of NPW reduced rat paw edema by ~50% after 6h of carrageenan administration. Hepatoprotective activity of NPW was also evaluated in vivo on CCl4-induced hepatotoxicity model in rats. NPW treatment (500 mg/kg/day for ten days) attenuated CCl4-induced increase in serum enzymes, viz. alanine and aspartate aminotransferases (ALT and AST) and bilirubin. Also, glutathione and superoxide dismutase (SOD)-levels were restored towards normalcy in the liver of CCl4-treated rats, indicating the hepatoprotective role of NPW, which was found to contain a fair amount of flavonoids, phenolics, and saponin constituents.

Authors+Show Affiliations

Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23827777

Citation

Debnath, Sukalyani, et al. "Inhibitory Effect of Nymphaea Pubescens Willd. Flower Extract On Carrageenan-induced Inflammation and CCl4-induced Hepatotoxicity in Rats." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 59, 2013, pp. 485-91.
Debnath S, Ghosh S, Hazra B. Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats. Food Chem Toxicol. 2013;59:485-91.
Debnath, S., Ghosh, S., & Hazra, B. (2013). Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 59, 485-91. https://doi.org/10.1016/j.fct.2013.06.036
Debnath S, Ghosh S, Hazra B. Inhibitory Effect of Nymphaea Pubescens Willd. Flower Extract On Carrageenan-induced Inflammation and CCl4-induced Hepatotoxicity in Rats. Food Chem Toxicol. 2013;59:485-91. PubMed PMID: 23827777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats. AU - Debnath,Sukalyani, AU - Ghosh,Subhalakshmi, AU - Hazra,Banasri, Y1 - 2013/07/01/ PY - 2013/03/14/received PY - 2013/05/21/revised PY - 2013/06/19/accepted PY - 2013/7/6/entrez PY - 2013/7/6/pubmed PY - 2014/3/29/medline KW - Anti-inflammatory KW - CCl4-induced hepatotoxicity KW - Carrageenan-induced paw edema KW - Free radical scavenger KW - Nymphaea pubescens KW - iNOS SP - 485 EP - 91 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 59 N2 - Nymphaea pubescens Willd. is used as ingredient of ethnic diet and folk medicine in South-East Asia. The water (NPW), methanol (NPM) and chloroform (NPC) extracts of N. pubescens flowers were investigated for NO·, O2·[-] and DPPH radical scavenging and iron chelating activities in vitro. NPW was found to be the most potent free radical scavenger (EC50<100 μg/mL) whereas NPC did not show EC50 at 500 μg/mL. Therefore, NPW was selected for further studies on anti-inflammatory and hepatoprotective activities, using standard in vitro and in vivo models. NPW exhibited inhibition of nitrogen radical generation in LPS-activated macrophages (IC50=75.5 μg/mL) through suppression of iNOS protein, with no associated toxicity in the cells. Further, 500 mg/kg of NPW reduced rat paw edema by ~50% after 6h of carrageenan administration. Hepatoprotective activity of NPW was also evaluated in vivo on CCl4-induced hepatotoxicity model in rats. NPW treatment (500 mg/kg/day for ten days) attenuated CCl4-induced increase in serum enzymes, viz. alanine and aspartate aminotransferases (ALT and AST) and bilirubin. Also, glutathione and superoxide dismutase (SOD)-levels were restored towards normalcy in the liver of CCl4-treated rats, indicating the hepatoprotective role of NPW, which was found to contain a fair amount of flavonoids, phenolics, and saponin constituents. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23827777/Inhibitory_effect_of_Nymphaea_pubescens_Willd__flower_extract_on_carrageenan_induced_inflammation_and_CCl4_induced_hepatotoxicity_in_rats_ DB - PRIME DP - Unbound Medicine ER -