Tags

Type your tag names separated by a space and hit enter

Interstitial cajal-like cells and bile lithogenicity in the pathogenesis of gall-stone disease.
Pol Przegl Chir 2013; 85(6):311-6PP

Abstract

Gall-stone disease constitutes a serious clinical problem and is the most frequent cause of elective cholecystectomies. There are many etiopatogenic factors however; lithogenic bile and its stasis due to gall-bladder hypomotility seem to be the most important. In recent years discovery of pacemaker function of Interstitial Cells of Cajal changed our understanding of smooth muscle physiology and helped to disclose many gastrointestinal motility disorders.

THE AIM OF THE STUDY

was identification and quantification of interstitial Cajal-like cells (ICLCs) in gall-bladder muscle wall from patients with cholelithiasis and in gall-stone-free controls, as well as determination of the relationship between the number of ICLCs and Cholesterol Saturation Index (CSI) of bile in both analyzed groups.

MATERIAL AND METHODS

20 patients operated for symptomatic cholelithiasis were enrolled into the study group. The control group consisted of 20 patients operated for pancreatic head tumors, with no pre- and intraoperative signs of gall-stones. Identification of ICLCs in the gall-bladder was performed by means of double immunofluorescence technique with anti c-Kit and anti-mast cell tryptase antibodies. Quantitative analysis was carried out under fluorescence microscopy conjoined with image analysis software. Bile samples were used for calculation of CSI.

RESULTS

ICLCs were detected within gall-bladder muscle wall. Number of ICLCs was statistically significantly lower in patients from the study group as compared to control. The study also revealed statistically significantly higher CSI in the study group.

CONCLUSIONS

The quantity of ICLCs is diminished in the gall-bladder from patients with cholelithiasis and there is negative correlation between the number of ICLCs and CSI of bile. Regarding the role of ICCs in regulation of GI tract motility, it appears that reduction in their number may be important etiopatogenic factor of cholelithiasis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23828411

Citation

Pasternak, Artur, et al. "Interstitial Cajal-like Cells and Bile Lithogenicity in the Pathogenesis of Gall-stone Disease." Polski Przeglad Chirurgiczny, vol. 85, no. 6, 2013, pp. 311-6.
Pasternak A, Matyja A, Gil K, et al. Interstitial cajal-like cells and bile lithogenicity in the pathogenesis of gall-stone disease. Pol Przegl Chir. 2013;85(6):311-6.
Pasternak, A., Matyja, A., Gil, K., Gajda, M., Tomaszewski, K. A., Gajda, M., ... Kulig, J. (2013). Interstitial cajal-like cells and bile lithogenicity in the pathogenesis of gall-stone disease. Polski Przeglad Chirurgiczny, 85(6), pp. 311-6. doi:10.2478/pjs-2013-0046.
Pasternak A, et al. Interstitial Cajal-like Cells and Bile Lithogenicity in the Pathogenesis of Gall-stone Disease. Pol Przegl Chir. 2013;85(6):311-6. PubMed PMID: 23828411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interstitial cajal-like cells and bile lithogenicity in the pathogenesis of gall-stone disease. AU - Pasternak,Artur, AU - Matyja,Andrzej, AU - Gil,Krzysztof, AU - Gajda,Mariusz, AU - Tomaszewski,Krzysztof A, AU - Gajda,Mariusz, AU - Tomaszewski,Krzysztof A, AU - Matyja,Maciej, AU - Walocha,Jerzy A, AU - Kulig,Jan, PY - 2013/7/6/entrez PY - 2013/7/6/pubmed PY - 2016/1/6/medline SP - 311 EP - 6 JF - Polski przeglad chirurgiczny JO - Pol Przegl Chir VL - 85 IS - 6 N2 - UNLABELLED: Gall-stone disease constitutes a serious clinical problem and is the most frequent cause of elective cholecystectomies. There are many etiopatogenic factors however; lithogenic bile and its stasis due to gall-bladder hypomotility seem to be the most important. In recent years discovery of pacemaker function of Interstitial Cells of Cajal changed our understanding of smooth muscle physiology and helped to disclose many gastrointestinal motility disorders. THE AIM OF THE STUDY: was identification and quantification of interstitial Cajal-like cells (ICLCs) in gall-bladder muscle wall from patients with cholelithiasis and in gall-stone-free controls, as well as determination of the relationship between the number of ICLCs and Cholesterol Saturation Index (CSI) of bile in both analyzed groups. MATERIAL AND METHODS: 20 patients operated for symptomatic cholelithiasis were enrolled into the study group. The control group consisted of 20 patients operated for pancreatic head tumors, with no pre- and intraoperative signs of gall-stones. Identification of ICLCs in the gall-bladder was performed by means of double immunofluorescence technique with anti c-Kit and anti-mast cell tryptase antibodies. Quantitative analysis was carried out under fluorescence microscopy conjoined with image analysis software. Bile samples were used for calculation of CSI. RESULTS: ICLCs were detected within gall-bladder muscle wall. Number of ICLCs was statistically significantly lower in patients from the study group as compared to control. The study also revealed statistically significantly higher CSI in the study group. CONCLUSIONS: The quantity of ICLCs is diminished in the gall-bladder from patients with cholelithiasis and there is negative correlation between the number of ICLCs and CSI of bile. Regarding the role of ICCs in regulation of GI tract motility, it appears that reduction in their number may be important etiopatogenic factor of cholelithiasis. SN - 2299-2847 UR - https://www.unboundmedicine.com/medline/citation/23828411/Interstitial_cajal_like_cells_and_bile_lithogenicity_in_the_pathogenesis_of_gall_stone_disease_ L2 - https://www.degruyter.com/doi/10.2478/pjs-2013-0046 DB - PRIME DP - Unbound Medicine ER -