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Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis.
Vet Immunol Immunopathol. 2013 Sep 01; 155(1-2):38-47.VI

Abstract

Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the β fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the α fragment of pollen allergen Amb a 1.0201.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23830203

Citation

Ognjenovic, Jana, et al. "Immunoproteomic Characterization of Ambrosia Artemisiifolia Pollen Allergens in Canine Atopic Dermatitis." Veterinary Immunology and Immunopathology, vol. 155, no. 1-2, 2013, pp. 38-47.
Ognjenovic J, Milcic-Matic N, Smiljanic K, et al. Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. Vet Immunol Immunopathol. 2013;155(1-2):38-47.
Ognjenovic, J., Milcic-Matic, N., Smiljanic, K., Vuckovic, O., Burazer, L., Popovic, N., Stanic-Vucinic, D., & Velickovic, T. C. (2013). Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. Veterinary Immunology and Immunopathology, 155(1-2), 38-47. https://doi.org/10.1016/j.vetimm.2013.06.005
Ognjenovic J, et al. Immunoproteomic Characterization of Ambrosia Artemisiifolia Pollen Allergens in Canine Atopic Dermatitis. Vet Immunol Immunopathol. 2013 Sep 1;155(1-2):38-47. PubMed PMID: 23830203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. AU - Ognjenovic,Jana, AU - Milcic-Matic,Natalija, AU - Smiljanic,Katarina, AU - Vuckovic,Olga, AU - Burazer,Lidija, AU - Popovic,Nikola, AU - Stanic-Vucinic,Dragana, AU - Velickovic,Tanja Cirkovic, Y1 - 2013/06/07/ PY - 2013/03/20/received PY - 2013/05/22/revised PY - 2013/06/03/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/4/4/medline KW - AD KW - Amb a 1 allergens KW - Ambrosia artemisiifolia pollen KW - CAD KW - CBB KW - Canine atopic dermatitis KW - Coomassie brilliant blue KW - Dog KW - ID KW - IDST KW - Immunoproteomics KW - LTQ KW - MS KW - PAA KW - PNU KW - Short ragweed KW - TTBS KW - Tris buffered saline with 0.1% Tween 20 (pH 7.4) KW - atopic dermatitis KW - canine atopic dermatitis KW - internal diameter KW - intradermal skin test KW - linear ion trap with quadrupole mass filter KW - mass spectroscopy KW - nLC–MS/MS KW - nano-liquid chromatography coupled to tandem mass spectroscopy KW - polyacrylamide KW - protein nitrogen unit SP - 38 EP - 47 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 155 IS - 1-2 N2 - Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the β fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the α fragment of pollen allergen Amb a 1.0201. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/23830203/Immunoproteomic_characterization_of_Ambrosia_artemisiifolia_pollen_allergens_in_canine_atopic_dermatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(13)00178-5 DB - PRIME DP - Unbound Medicine ER -