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Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea.
Food Chem Toxicol. 2013 Sep; 59:527-33.FC

Abstract

An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50-200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H₂O₂) treatment. Compared with the H₂O₂ treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties.

Authors+Show Affiliations

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23831194

Citation

Fan, Jiang Ping, et al. "Free Radical Scavenging and Anti-oxidative Activities of an Ethanol-soluble Pigment Extract Prepared From Fermented Zijuan Pu-erh Tea." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 59, 2013, pp. 527-33.
Fan JP, Fan C, Dong WM, et al. Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea. Food Chem Toxicol. 2013;59:527-33.
Fan, J. P., Fan, C., Dong, W. M., Gao, B., Yuan, W., & Gong, J. S. (2013). Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 59, 527-33. https://doi.org/10.1016/j.fct.2013.06.047
Fan JP, et al. Free Radical Scavenging and Anti-oxidative Activities of an Ethanol-soluble Pigment Extract Prepared From Fermented Zijuan Pu-erh Tea. Food Chem Toxicol. 2013;59:527-33. PubMed PMID: 23831194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea. AU - Fan,Jiang Ping, AU - Fan,Chong, AU - Dong,Wen Min, AU - Gao,Bin, AU - Yuan,Wei, AU - Gong,Jia Shun, Y1 - 2013/07/03/ PY - 2013/02/15/received PY - 2013/06/19/revised PY - 2013/06/23/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/3/29/medline KW - Anti-oxidation KW - Ethanol-soluble pigment KW - Free radical KW - Zijuan Pu-erh tea SP - 527 EP - 33 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 59 N2 - An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography-tandem mass spectroscopy (HPLC-MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50-200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H₂O₂) treatment. Compared with the H₂O₂ treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23831194/Free_radical_scavenging_and_anti_oxidative_activities_of_an_ethanol_soluble_pigment_extract_prepared_from_fermented_Zijuan_Pu_erh_tea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(13)00426-2 DB - PRIME DP - Unbound Medicine ER -