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CCL18 in serum, BAL fluid and alveolar macrophage culture supernatant in interstitial lung diseases.
Respir Med. 2013 Sep; 107(9):1444-52.RM

Abstract

BACKGROUND

CCL18 is a CC chemokine produced mainly by antigen-presenting cells, and is chemotactic predominantly for T-lymphocytes. CCL18 can stimulate pulmonary fibroblasts and increase the collagen production in vitro.

OBJECTIVES

This study aimed to compare the CCL18 levels in a variety of human biological fluids between various interstitial lung diseases (ILDs), and to reveal potential correlations with BAL cell differentials.

METHODS

Serum and bronchoalveolar lavage fluid (BALF) samples were collected from 199 patients with idiopathic pulmonary fibrosis (IPF), idiopathic non-specific interstitial pneumonia (iNSIP), respiratory bronchiolitis interstitial lung disease/desquamative interstitial pneumonia (RB-ILD/DIP), cryptogenic organizing pneumonia (COP), hypersensitivity pneumonitis (HP) or sarcoidosis. Alveolar macrophage (AM) culture was performed in 44 patients with IPF, iNSIP, COP, HP, sarcoidosis or non-ILDs. The CCL18 levels in serum, BALF and AM culture supernatant were measured with ELISA.

RESULTS

Both serum and BALF CCL18 levels in all ILDs were higher than in controls (all p < 0.005). In HP, CCL18 serum levels were the highest of all ILDs, and its BALF levels were significantly higher than in other ILDs except iNSIP. The BALF CCL18 levels markedly correlated with BAL cell differentials, especially with the percentage of BAL lymphocytes. In AM culture supernatant, the spontaneous CCL18 production was higher in HP and COP than in IPF and controls.

CONCLUSION

CCL18 levels in serum, BALF and AM culture supernatant are markedly increased in various inflammatory and fibrotic ILDs. However, the CCL18 level being highest in HP among the investigated ILDs suggests that CCL18 may be more profoundly involved in inflammatory immune responses.

Authors+Show Affiliations

Department of Pneumology and Allergology, Ruhrlandklinik, University Hospital, University Duisburg-Essen, Tueschener Weg 40, D-45239 Essen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23831213

Citation

Cai, Miaotian, et al. "CCL18 in Serum, BAL Fluid and Alveolar Macrophage Culture Supernatant in Interstitial Lung Diseases." Respiratory Medicine, vol. 107, no. 9, 2013, pp. 1444-52.
Cai M, Bonella F, He X, et al. CCL18 in serum, BAL fluid and alveolar macrophage culture supernatant in interstitial lung diseases. Respir Med. 2013;107(9):1444-52.
Cai, M., Bonella, F., He, X., Sixt, S. U., Sarria, R., Guzman, J., & Costabel, U. (2013). CCL18 in serum, BAL fluid and alveolar macrophage culture supernatant in interstitial lung diseases. Respiratory Medicine, 107(9), 1444-52. https://doi.org/10.1016/j.rmed.2013.06.004
Cai M, et al. CCL18 in Serum, BAL Fluid and Alveolar Macrophage Culture Supernatant in Interstitial Lung Diseases. Respir Med. 2013;107(9):1444-52. PubMed PMID: 23831213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CCL18 in serum, BAL fluid and alveolar macrophage culture supernatant in interstitial lung diseases. AU - Cai,Miaotian, AU - Bonella,Francesco, AU - He,Xuan, AU - Sixt,Stephan U, AU - Sarria,Rafael, AU - Guzman,Josune, AU - Costabel,Ulrich, Y1 - 2013/07/05/ PY - 2013/02/06/received PY - 2013/05/11/revised PY - 2013/06/03/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/3/26/medline KW - AM KW - AMAC KW - ATS/ERS KW - AaO(2) KW - American Thoracic Society/European Respiratory Society KW - BALF KW - CC chemokine ligand KW - CCL KW - CCL18 KW - COP KW - DC-CK KW - DIP KW - DLco KW - ELISA KW - FEV KW - Fibrosis KW - HP KW - HRCT KW - Hypersensitivity pneumonitis KW - IFN KW - IIP KW - IL KW - ILD KW - IPF KW - Idiopathic pulmonary fibrosis KW - Inflammation KW - Interstitial lung disease KW - LPS KW - MIP KW - NSIP KW - PARC KW - RB-ILD KW - SaO(2) KW - T-helper KW - TNF KW - Th KW - VC KW - alternative macrophage activation-associated CC chemokine KW - alveolar macrophage KW - alveolar-arterial PO(2) difference KW - bronchoalveolar lavage fluid KW - cryptogenic organizing pneumonia KW - dendritic cell-chemokine KW - desquamative interstitial pneumonia KW - diffusing capacity of the lung for carbon monoxide KW - enzyme-linked immunosorbent assay KW - forced expiratory volume KW - high-resolution computed tomography KW - hypersensitivity pneumonitis KW - idiopathic interstitial pneumonia KW - idiopathic pulmonary fibrosis KW - interferon KW - interleukin KW - interstitial lung disease KW - lipopolysaccharide KW - monocyte inflammatory protein KW - non-specific interstitial pneumonia KW - oxygen percent saturation (arterial) KW - pulmonary and activity-regulated chemokine KW - respiratory bronchiolitis interstitial lung disease KW - tumor necrosis factor KW - vital capacity SP - 1444 EP - 52 JF - Respiratory medicine JO - Respir Med VL - 107 IS - 9 N2 - BACKGROUND: CCL18 is a CC chemokine produced mainly by antigen-presenting cells, and is chemotactic predominantly for T-lymphocytes. CCL18 can stimulate pulmonary fibroblasts and increase the collagen production in vitro. OBJECTIVES: This study aimed to compare the CCL18 levels in a variety of human biological fluids between various interstitial lung diseases (ILDs), and to reveal potential correlations with BAL cell differentials. METHODS: Serum and bronchoalveolar lavage fluid (BALF) samples were collected from 199 patients with idiopathic pulmonary fibrosis (IPF), idiopathic non-specific interstitial pneumonia (iNSIP), respiratory bronchiolitis interstitial lung disease/desquamative interstitial pneumonia (RB-ILD/DIP), cryptogenic organizing pneumonia (COP), hypersensitivity pneumonitis (HP) or sarcoidosis. Alveolar macrophage (AM) culture was performed in 44 patients with IPF, iNSIP, COP, HP, sarcoidosis or non-ILDs. The CCL18 levels in serum, BALF and AM culture supernatant were measured with ELISA. RESULTS: Both serum and BALF CCL18 levels in all ILDs were higher than in controls (all p < 0.005). In HP, CCL18 serum levels were the highest of all ILDs, and its BALF levels were significantly higher than in other ILDs except iNSIP. The BALF CCL18 levels markedly correlated with BAL cell differentials, especially with the percentage of BAL lymphocytes. In AM culture supernatant, the spontaneous CCL18 production was higher in HP and COP than in IPF and controls. CONCLUSION: CCL18 levels in serum, BALF and AM culture supernatant are markedly increased in various inflammatory and fibrotic ILDs. However, the CCL18 level being highest in HP among the investigated ILDs suggests that CCL18 may be more profoundly involved in inflammatory immune responses. SN - 1532-3064 UR - https://www.unboundmedicine.com/medline/citation/23831213/CCL18_in_serum_BAL_fluid_and_alveolar_macrophage_culture_supernatant_in_interstitial_lung_diseases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(13)00216-3 DB - PRIME DP - Unbound Medicine ER -