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Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration.
Anal Bioanal Chem. 2013 Sep; 405(23):7269-79.AB

Abstract

Oral fluid (OF) is a valuable biological alternative for clinical and forensic drug testing. Evaluating OF to plasma (OF/P) cannabinoid ratios provides important pharmacokinetic data on the disposition of drug and factors influencing partition between matrices. Eleven chronic cannabis smokers resided on a closed research unit for 51 days. There were four 5-day sessions of 0, 30, 60, and 120 mg oral ∆(9)-tetrahydrocannabinol (THC)/day followed by a five-puff smoked cannabis challenge on Day 5. Each session was separated by 9 days ad libitum cannabis smoking. OF and plasma specimens were analyzed for THC and metabolites. During ad libitum smoking, OF/P THC ratios were high (median, 6.1; range, 0.2-348.5) within 1 h after last smoking, decreasing to 0.1-20.7 (median, 2.1) by 13.0-17.1 h. OF/P THC ratios also decreased during 5-days oral THC dosing, and after the smoked cannabis challenge, median OF/P THC ratios decreased from 1.4 to 5.5 (0.04-245.6) at 0.25 h to 0.12 to 0.17 (0.04-5.1) at 10.5 h post-smoking. In other studies, longer exposure to more potent cannabis smoke and oromucosal cannabis spray was associated with increased OF/P THC peak ratios. Median OF/P 11-nor-9-carboxy-THC (THCCOOH) ratios were 0.3-2.5 (range, 0.1-14.7) ng/μg, much more consistent in various dosing conditions over time. OF/P THC, but not THCCOOH, ratios were significantly influenced by oral cavity contamination after smoking or oromucosal spray of cannabinoid products, followed by time-dependent decreases. Establishing relationships between OF and plasma cannabinoid concentrations is essential for making inferences of impairment or other clinical outcomes from OF concentrations.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Biomedical Research Center, Baltimore, MD 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

23831756

Citation

Lee, Dayong, et al. "Oral Fluid/plasma Cannabinoid Ratios Following Controlled Oral THC and Smoked Cannabis Administration." Analytical and Bioanalytical Chemistry, vol. 405, no. 23, 2013, pp. 7269-79.
Lee D, Vandrey R, Milman G, et al. Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration. Anal Bioanal Chem. 2013;405(23):7269-79.
Lee, D., Vandrey, R., Milman, G., Bergamaschi, M., Mendu, D. R., Murray, J. A., Barnes, A. J., & Huestis, M. A. (2013). Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration. Analytical and Bioanalytical Chemistry, 405(23), 7269-79. https://doi.org/10.1007/s00216-013-7159-8
Lee D, et al. Oral Fluid/plasma Cannabinoid Ratios Following Controlled Oral THC and Smoked Cannabis Administration. Anal Bioanal Chem. 2013;405(23):7269-79. PubMed PMID: 23831756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration. AU - Lee,Dayong, AU - Vandrey,Ryan, AU - Milman,Garry, AU - Bergamaschi,Mateus, AU - Mendu,Damodara R, AU - Murray,Jeannie A, AU - Barnes,Allan J, AU - Huestis,Marilyn A, Y1 - 2013/07/06/ PY - 2013/05/08/received PY - 2013/06/18/accepted PY - 2013/06/17/revised PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/10/28/medline SP - 7269 EP - 79 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 405 IS - 23 N2 - Oral fluid (OF) is a valuable biological alternative for clinical and forensic drug testing. Evaluating OF to plasma (OF/P) cannabinoid ratios provides important pharmacokinetic data on the disposition of drug and factors influencing partition between matrices. Eleven chronic cannabis smokers resided on a closed research unit for 51 days. There were four 5-day sessions of 0, 30, 60, and 120 mg oral ∆(9)-tetrahydrocannabinol (THC)/day followed by a five-puff smoked cannabis challenge on Day 5. Each session was separated by 9 days ad libitum cannabis smoking. OF and plasma specimens were analyzed for THC and metabolites. During ad libitum smoking, OF/P THC ratios were high (median, 6.1; range, 0.2-348.5) within 1 h after last smoking, decreasing to 0.1-20.7 (median, 2.1) by 13.0-17.1 h. OF/P THC ratios also decreased during 5-days oral THC dosing, and after the smoked cannabis challenge, median OF/P THC ratios decreased from 1.4 to 5.5 (0.04-245.6) at 0.25 h to 0.12 to 0.17 (0.04-5.1) at 10.5 h post-smoking. In other studies, longer exposure to more potent cannabis smoke and oromucosal cannabis spray was associated with increased OF/P THC peak ratios. Median OF/P 11-nor-9-carboxy-THC (THCCOOH) ratios were 0.3-2.5 (range, 0.1-14.7) ng/μg, much more consistent in various dosing conditions over time. OF/P THC, but not THCCOOH, ratios were significantly influenced by oral cavity contamination after smoking or oromucosal spray of cannabinoid products, followed by time-dependent decreases. Establishing relationships between OF and plasma cannabinoid concentrations is essential for making inferences of impairment or other clinical outcomes from OF concentrations. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/23831756/Oral_fluid/plasma_cannabinoid_ratios_following_controlled_oral_THC_and_smoked_cannabis_administration_ L2 - https://dx.doi.org/10.1007/s00216-013-7159-8 DB - PRIME DP - Unbound Medicine ER -