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Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene.
Bioresour Technol 2013; 143:531-7BT

Abstract

This study aims to investigate whether overexpressing the trehalose biosynthetic gene, otsBA operon, in β-carotene-producing recombinant Escherichia coli protects cells from toxic impurities in crude glycerol. The concentrations of potassium and methanol in crude glycerol were too low to inhibit cell growth. Cell growth and production in control cell culture were inhibited significantly in the presence of a small amount of crude fatty acids. Peroxides were generated in the presence of crude fatty acids during autoclaving and, thus, the inhibitory effect of crude fatty acids was caused primarily by these peroxides. Engineered cells overexpressing otsBA tolerated crude fatty acids (≤42 wet-g/L), methanol (≤7.5 g/L), and t-BuOOH (≤60 μM) in separate experiments and tolerated up to 60 g/L crude glycerol. These results demonstrate that overexpressing otsBA endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use.

Authors+Show Affiliations

Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju 660-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23831895

Citation

Nguyen, Anh Do Quynh, et al. "Improved Tolerance of Recombinant Escherichia Coli to the Toxicity of Crude Glycerol By Overexpressing Trehalose Biosynthetic Genes (otsBA) for the Production of Β-carotene." Bioresource Technology, vol. 143, 2013, pp. 531-7.
Nguyen Ado Q, Kim YG, Kim SB, et al. Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene. Bioresour Technol. 2013;143:531-7.
Nguyen, A. d. o. . Q., Kim, Y. G., Kim, S. B., & Kim, C. J. (2013). Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene. Bioresource Technology, 143, pp. 531-7. doi:10.1016/j.biortech.2013.06.034.
Nguyen Ado Q, et al. Improved Tolerance of Recombinant Escherichia Coli to the Toxicity of Crude Glycerol By Overexpressing Trehalose Biosynthetic Genes (otsBA) for the Production of Β-carotene. Bioresour Technol. 2013;143:531-7. PubMed PMID: 23831895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene. AU - Nguyen,Anh Do Quynh, AU - Kim,You Geun, AU - Kim,Sung Bae, AU - Kim,Chang-Joon, Y1 - 2013/06/19/ PY - 2013/04/02/received PY - 2013/06/10/revised PY - 2013/06/11/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/3/1/medline KW - Crude glycerol KW - Recombinant Escherichia coli KW - Tolerance KW - Trehalose biosynthetic operon KW - β-Carotene SP - 531 EP - 7 JF - Bioresource technology JO - Bioresour. Technol. VL - 143 N2 - This study aims to investigate whether overexpressing the trehalose biosynthetic gene, otsBA operon, in β-carotene-producing recombinant Escherichia coli protects cells from toxic impurities in crude glycerol. The concentrations of potassium and methanol in crude glycerol were too low to inhibit cell growth. Cell growth and production in control cell culture were inhibited significantly in the presence of a small amount of crude fatty acids. Peroxides were generated in the presence of crude fatty acids during autoclaving and, thus, the inhibitory effect of crude fatty acids was caused primarily by these peroxides. Engineered cells overexpressing otsBA tolerated crude fatty acids (≤42 wet-g/L), methanol (≤7.5 g/L), and t-BuOOH (≤60 μM) in separate experiments and tolerated up to 60 g/L crude glycerol. These results demonstrate that overexpressing otsBA endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/23831895/Improved_tolerance_of_recombinant_Escherichia_coli_to_the_toxicity_of_crude_glycerol_by_overexpressing_trehalose_biosynthetic_genes__otsBA__for_the_production_of_β_carotene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(13)00953-X DB - PRIME DP - Unbound Medicine ER -