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Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes.
Luminescence. 2014 Jun; 29(4):338-43.L

Abstract

A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N = 3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N = 3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n = 11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results.

Authors+Show Affiliations

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23832716

Citation

Zhou, Yuan, et al. "Chemiluminescence Immunoassay for the Rapid and Sensitive Detection of Antibody Against Porcine Parvovirus By Using Horseradish Peroxidase/detection Antibody-coated Gold Nanoparticles as Nanoprobes." Luminescence : the Journal of Biological and Chemical Luminescence, vol. 29, no. 4, 2014, pp. 338-43.
Zhou Y, Zhou T, Zhou R, et al. Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes. Luminescence. 2014;29(4):338-43.
Zhou, Y., Zhou, T., Zhou, R., & Hu, Y. (2014). Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes. Luminescence : the Journal of Biological and Chemical Luminescence, 29(4), 338-43. https://doi.org/10.1002/bio.2549
Zhou Y, et al. Chemiluminescence Immunoassay for the Rapid and Sensitive Detection of Antibody Against Porcine Parvovirus By Using Horseradish Peroxidase/detection Antibody-coated Gold Nanoparticles as Nanoprobes. Luminescence. 2014;29(4):338-43. PubMed PMID: 23832716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes. AU - Zhou,Yuan, AU - Zhou,Tao, AU - Zhou,Rui, AU - Hu,Yonggang, Y1 - 2013/07/05/ PY - 2012/12/13/received PY - 2013/05/01/revised PY - 2013/05/10/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/12/23/medline KW - chemiluminescence immunoassay KW - enzymatic amplification KW - gold nanoparticles KW - porcine parvovirus SP - 338 EP - 43 JF - Luminescence : the journal of biological and chemical luminescence JO - Luminescence VL - 29 IS - 4 N2 - A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N = 3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N = 3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n = 11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results. SN - 1522-7243 UR - https://www.unboundmedicine.com/medline/citation/23832716/Chemiluminescence_immunoassay_for_the_rapid_and_sensitive_detection_of_antibody_against_porcine_parvovirus_by_using_horseradish_peroxidase/detection_antibody_coated_gold_nanoparticles_as_nanoprobes_ L2 - https://doi.org/10.1002/bio.2549 DB - PRIME DP - Unbound Medicine ER -