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Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples.
Biomed Chromatogr. 2013 Nov; 27(11):1481-8.BC

Abstract

This study presents the use of molecularly imprinted polymer (MIP) as packing material for microextraction by packed syringe (MEPS) to achieve higher extraction selectivity. Pentycaine was used as template for MIP. Development and validation of the determination of lidocaine, ropivacaine, mepivacaine and bupivacaine in human plasma and urine samples utilizing MIP-MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. The MEPS MIP-cartridge could be used for 100 extractions before it was discarded. The extraction recovery ranged from 60 to 80%. The correlation coefficients values were >0.999 for all assays using lidocaine, ropivacaine, mepivacaine and bupivacaine in the calibration range 5-2000 nmol/L. The accuracy of the studied compounds, given as a percentage variation from the nominal concentration values, ranged from -4.9 to 8.4% using plasma and urine samples. The between-batch precision, given as the relative standard deviation, at three different concentrations (quality control samples) was ranged from -4.7 to 14.0% and from 1.8 to 12.7% in plasma and urine, respectively. The lower limit of quantification and limit of detection of the studied substances were 5.0 and 1.0 nm, respectively.

Authors+Show Affiliations

Department of Analytical Chemistry, Stockholm University, SE-10691, Stockholm, Sweden; Faculty of Chemistry, Bu Ali-Sina University, Hamedan, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

23832864

Citation

Daryanavard, Seyed Mosayeb, et al. "Molecularly Imprinted Polymer in Microextraction By Packed Sorbent for the Simultaneous Determination of Local Anesthetics: Lidocaine, Ropivacaine, Mepivacaine and Bupivacaine in Plasma and Urine Samples." Biomedical Chromatography : BMC, vol. 27, no. 11, 2013, pp. 1481-8.
Daryanavard SM, Jeppsson-Dadoun A, Andersson LI, et al. Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples. Biomed Chromatogr. 2013;27(11):1481-8.
Daryanavard, S. M., Jeppsson-Dadoun, A., Andersson, L. I., Hashemi, M., Colmsjö, A., & Abdel-Rehim, M. (2013). Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples. Biomedical Chromatography : BMC, 27(11), 1481-8. https://doi.org/10.1002/bmc.2946
Daryanavard SM, et al. Molecularly Imprinted Polymer in Microextraction By Packed Sorbent for the Simultaneous Determination of Local Anesthetics: Lidocaine, Ropivacaine, Mepivacaine and Bupivacaine in Plasma and Urine Samples. Biomed Chromatogr. 2013;27(11):1481-8. PubMed PMID: 23832864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics: lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples. AU - Daryanavard,Seyed Mosayeb, AU - Jeppsson-Dadoun,Amin, AU - Andersson,Lars I, AU - Hashemi,Mahdi, AU - Colmsjö,Anders, AU - Abdel-Rehim,Mohamed, Y1 - 2013/07/05/ PY - 2013/04/01/received PY - 2013/04/29/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2014/5/16/medline KW - LC-MS/MS KW - MEPS KW - MIP KW - local anesthetic drugs KW - plasma and urine samples SP - 1481 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 27 IS - 11 N2 - This study presents the use of molecularly imprinted polymer (MIP) as packing material for microextraction by packed syringe (MEPS) to achieve higher extraction selectivity. Pentycaine was used as template for MIP. Development and validation of the determination of lidocaine, ropivacaine, mepivacaine and bupivacaine in human plasma and urine samples utilizing MIP-MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. The MEPS MIP-cartridge could be used for 100 extractions before it was discarded. The extraction recovery ranged from 60 to 80%. The correlation coefficients values were >0.999 for all assays using lidocaine, ropivacaine, mepivacaine and bupivacaine in the calibration range 5-2000 nmol/L. The accuracy of the studied compounds, given as a percentage variation from the nominal concentration values, ranged from -4.9 to 8.4% using plasma and urine samples. The between-batch precision, given as the relative standard deviation, at three different concentrations (quality control samples) was ranged from -4.7 to 14.0% and from 1.8 to 12.7% in plasma and urine, respectively. The lower limit of quantification and limit of detection of the studied substances were 5.0 and 1.0 nm, respectively. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/23832864/Molecularly_imprinted_polymer_in_microextraction_by_packed_sorbent_for_the_simultaneous_determination_of_local_anesthetics:_lidocaine_ropivacaine_mepivacaine_and_bupivacaine_in_plasma_and_urine_samples_ L2 - https://doi.org/10.1002/bmc.2946 DB - PRIME DP - Unbound Medicine ER -