Tags

Type your tag names separated by a space and hit enter

Indocyanine green-loaded perfluorocarbon nanoemulsions for bimodal (19)F-magnetic resonance/nearinfrared fluorescence imaging and subsequent phototherapy.
Quant Imaging Med Surg. 2013 Jun; 3(3):132-40.QI

Abstract

We have developed an indocyanine green-loaded perfluorocarbon (ICG/PFCE) nanoemulsion as a multifunctional theranostic nanomedicine which enables not only (19)F magnetic resonance (MR)/near-infrared fluorescence (NIRF) bimodal imaging but also subsequent photodynamic/photothermal dual therapy of cancer. The hydrodynamic size of ICG/PFCE nanoemulsions was 164.2 nm. The stability of indocyanine green (ICG) in aqueous solution was significantly improved when loaded on perfluorocarbon nanoemulsions. In addition, ICG/PFCE nanoemulsions showed good dispersion stability in aqueous media containing 10% fetal bovine serum, for at least 14 days. (19)F-MRI of ICG/PFCE nanoemulsions showed that the signal intensity increased with increasing nanoemulsion concentration with no signal observed from the surrounding background. Using NIRF imaging with perfluorocarbon nanoemulsion alone, without ICG, did not produce NIRF, while clear and bright fluorescent images were obtained with ICG/PFCE nanoemulsions at 10-µM ICG equivalent. The capacity of ICG-loaded nanoemulsions to generate heat following light irradiation by using an 810-nm laser was comparable to that of free ICG, while singlet oxygen generation of ICG-loaded nanoemulsions was significantly better than that of free ICG. In vitro cytotoxicity tests and fluorescence microscopy confirmed biocompatibility of the nanoemulsion. Upon light irradiation, U87MG glioblastoma cells incubated with ICG/PFCE nanoemulsions underwent necrotic cell death. The therapeutic mechanism during light illumination appears to be mainly due to the photodynamic effect at lower ICG concentrations, whilst the photothermal effect became more obvious at increased ICG concentrations, enabling combined photodynamic/photothermal therapy of cancer cells.

Authors+Show Affiliations

Molecular Imaging and Therapy Branch, National Cancer Center, 323 Ilsan-ro, Goyang-si, Gyeonggi-do 410-769, Korea;No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23833726

Citation

Wang, Yuan-Guo, et al. "Indocyanine Green-loaded Perfluorocarbon Nanoemulsions for Bimodal (19)F-magnetic Resonance/nearinfrared Fluorescence Imaging and Subsequent Phototherapy." Quantitative Imaging in Medicine and Surgery, vol. 3, no. 3, 2013, pp. 132-40.
Wang YG, Kim H, Mun S, et al. Indocyanine green-loaded perfluorocarbon nanoemulsions for bimodal (19)F-magnetic resonance/nearinfrared fluorescence imaging and subsequent phototherapy. Quant Imaging Med Surg. 2013;3(3):132-40.
Wang, Y. G., Kim, H., Mun, S., Kim, D., & Choi, Y. (2013). Indocyanine green-loaded perfluorocarbon nanoemulsions for bimodal (19)F-magnetic resonance/nearinfrared fluorescence imaging and subsequent phototherapy. Quantitative Imaging in Medicine and Surgery, 3(3), 132-40. https://doi.org/10.3978/j.issn.2223-4292.2013.06.03
Wang YG, et al. Indocyanine Green-loaded Perfluorocarbon Nanoemulsions for Bimodal (19)F-magnetic Resonance/nearinfrared Fluorescence Imaging and Subsequent Phototherapy. Quant Imaging Med Surg. 2013;3(3):132-40. PubMed PMID: 23833726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indocyanine green-loaded perfluorocarbon nanoemulsions for bimodal (19)F-magnetic resonance/nearinfrared fluorescence imaging and subsequent phototherapy. AU - Wang,Yuan-Guo, AU - Kim,Hyunjin, AU - Mun,Saehun, AU - Kim,Daehong, AU - Choi,Yongdoo, PY - 2013/06/01/received PY - 2013/06/21/accepted PY - 2013/7/9/entrez PY - 2013/7/9/pubmed PY - 2013/7/9/medline KW - 19F-magnetic resonance imaging KW - Indocyanine green KW - near-infrared fluorescence imaging KW - perfluorocarbon KW - photodynamic therapy KW - photothermal therapy SP - 132 EP - 40 JF - Quantitative imaging in medicine and surgery JO - Quant Imaging Med Surg VL - 3 IS - 3 N2 - We have developed an indocyanine green-loaded perfluorocarbon (ICG/PFCE) nanoemulsion as a multifunctional theranostic nanomedicine which enables not only (19)F magnetic resonance (MR)/near-infrared fluorescence (NIRF) bimodal imaging but also subsequent photodynamic/photothermal dual therapy of cancer. The hydrodynamic size of ICG/PFCE nanoemulsions was 164.2 nm. The stability of indocyanine green (ICG) in aqueous solution was significantly improved when loaded on perfluorocarbon nanoemulsions. In addition, ICG/PFCE nanoemulsions showed good dispersion stability in aqueous media containing 10% fetal bovine serum, for at least 14 days. (19)F-MRI of ICG/PFCE nanoemulsions showed that the signal intensity increased with increasing nanoemulsion concentration with no signal observed from the surrounding background. Using NIRF imaging with perfluorocarbon nanoemulsion alone, without ICG, did not produce NIRF, while clear and bright fluorescent images were obtained with ICG/PFCE nanoemulsions at 10-µM ICG equivalent. The capacity of ICG-loaded nanoemulsions to generate heat following light irradiation by using an 810-nm laser was comparable to that of free ICG, while singlet oxygen generation of ICG-loaded nanoemulsions was significantly better than that of free ICG. In vitro cytotoxicity tests and fluorescence microscopy confirmed biocompatibility of the nanoemulsion. Upon light irradiation, U87MG glioblastoma cells incubated with ICG/PFCE nanoemulsions underwent necrotic cell death. The therapeutic mechanism during light illumination appears to be mainly due to the photodynamic effect at lower ICG concentrations, whilst the photothermal effect became more obvious at increased ICG concentrations, enabling combined photodynamic/photothermal therapy of cancer cells. SN - 2223-4292 UR - https://www.unboundmedicine.com/medline/citation/23833726/Indocyanine_green_loaded_perfluorocarbon_nanoemulsions_for_bimodal__19_F_magnetic_resonance/nearinfrared_fluorescence_imaging_and_subsequent_phototherapy_ L2 - https://doi.org/10.3978/j.issn.2223-4292.2013.06.03 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.