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An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge.
Virol J. 2013 Jul 09; 10:227.VJ

Abstract

BACKGROUND

Vaccination is considered as the most effective preventive method to control influenza. The hallmark of influenza virus is the remarkable variability of its major surface glycoproteins, HA and NA, which allows the virus to evade existing anti-influenza immunity in the target population. So it is necessary to develop a novel vaccine to control animal influenza virus. Also we know that the ectodomain of influenza matrix protein 2 (M2e) is highly conserved in animal influenza A viruses, so a vaccine based on the M2e could avoid several drawbacks of the traditional vaccines. In this study we designed a novel tetra-branched multiple antigenic peptide (MAP) based vaccine, which was constructed by fusing four copies of M2e to one copy of foreign T helper (Th) cell epitope, and then investigated its immune responses.

RESULTS

Our results show that the M2e-MAP induced strong M2e-specific IgG antibody,which responses following 2 doses immunization in the presence of Freunds' adjuvant. M2e-MAP vaccination limited viral replication substantially. Also it could attenuate histopathological damage in the lungs of challenged mice and counteracted weight loss. M2e-MAP-based vaccine protected immunized mice against the lethal challenge with PR8 virus.

CONCLUSIONS

Based on these findings, M2e-MAP-based vaccine seemed to provide useful information for the research of M2e-based influenza vaccine. Also it show huge potential to study vaccines for other similarly viruses.

Authors+Show Affiliations

Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23834899

Citation

Ma, Ji-Hong, et al. "An M2e-based Synthetic Peptide Vaccine for Influenza a Virus Confers Heterosubtypic Protection From Lethal Virus Challenge." Virology Journal, vol. 10, 2013, p. 227.
Ma JH, Yang FR, Yu H, et al. An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge. Virol J. 2013;10:227.
Ma, J. H., Yang, F. R., Yu, H., Zhou, Y. J., Li, G. X., Huang, M., Wen, F., & Tong, G. (2013). An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge. Virology Journal, 10, 227. https://doi.org/10.1186/1743-422X-10-227
Ma JH, et al. An M2e-based Synthetic Peptide Vaccine for Influenza a Virus Confers Heterosubtypic Protection From Lethal Virus Challenge. Virol J. 2013 Jul 9;10:227. PubMed PMID: 23834899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge. AU - Ma,Ji-Hong, AU - Yang,Fu-Ru, AU - Yu,Hai, AU - Zhou,Yan-Jun, AU - Li,Guo-Xin, AU - Huang,Meng, AU - Wen,Feng, AU - Tong,Guangzhi, Y1 - 2013/07/09/ PY - 2012/11/10/received PY - 2013/07/04/accepted PY - 2013/7/10/entrez PY - 2013/7/10/pubmed PY - 2014/1/23/medline SP - 227 EP - 227 JF - Virology journal JO - Virol J VL - 10 N2 - BACKGROUND: Vaccination is considered as the most effective preventive method to control influenza. The hallmark of influenza virus is the remarkable variability of its major surface glycoproteins, HA and NA, which allows the virus to evade existing anti-influenza immunity in the target population. So it is necessary to develop a novel vaccine to control animal influenza virus. Also we know that the ectodomain of influenza matrix protein 2 (M2e) is highly conserved in animal influenza A viruses, so a vaccine based on the M2e could avoid several drawbacks of the traditional vaccines. In this study we designed a novel tetra-branched multiple antigenic peptide (MAP) based vaccine, which was constructed by fusing four copies of M2e to one copy of foreign T helper (Th) cell epitope, and then investigated its immune responses. RESULTS: Our results show that the M2e-MAP induced strong M2e-specific IgG antibody,which responses following 2 doses immunization in the presence of Freunds' adjuvant. M2e-MAP vaccination limited viral replication substantially. Also it could attenuate histopathological damage in the lungs of challenged mice and counteracted weight loss. M2e-MAP-based vaccine protected immunized mice against the lethal challenge with PR8 virus. CONCLUSIONS: Based on these findings, M2e-MAP-based vaccine seemed to provide useful information for the research of M2e-based influenza vaccine. Also it show huge potential to study vaccines for other similarly viruses. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/23834899/An_M2e_based_synthetic_peptide_vaccine_for_influenza_A_virus_confers_heterosubtypic_protection_from_lethal_virus_challenge_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-10-227 DB - PRIME DP - Unbound Medicine ER -