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Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew.
Chromosome Res. 2013 Jul; 21(4):419-32.CR

Abstract

Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat-rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat-rye T4BL·4RL and T7AS·4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgt isolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7, Pm8, Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.

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Publisher Full Text (DOI)

Authors+Show Affiliations

An D
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology IGDB, Chinese Academy of Sciences CAS, Shijiazhuang 050021, China. dgan@sjziam.ac.cn
Zheng Q
No affiliation info available
Zhou Y
No affiliation info available
Ma P
No affiliation info available
Lv Z
No affiliation info available
Li L
No affiliation info available
Li B
No affiliation info available
Luo Q
No affiliation info available
Xu H
No affiliation info available
Xu Y
No affiliation info available

MeSH

Chromosome MappingChromosomes, PlantCytogeneticsDisease ResistanceIn Situ Hybridization, FluorescenceMicrosatellite RepeatsPlant DiseasesPlant ProteinsSecaleTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23836161

Citation

An, Diaoguo, et al. "Molecular Cytogenetic Characterization of a New Wheat-rye 4R Chromosome Translocation Line Resistant to Powdery Mildew." Chromosome Research : an International Journal On the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology, vol. 21, no. 4, 2013, pp. 419-32.
An D, Zheng Q, Zhou Y, et al. Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew. Chromosome Res. 2013;21(4):419-32.
An, D., Zheng, Q., Zhou, Y., Ma, P., Lv, Z., Li, L., Li, B., Luo, Q., Xu, H., & Xu, Y. (2013). Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew. Chromosome Research : an International Journal On the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology, 21(4), 419-32. https://doi.org/10.1007/s10577-013-9366-8
An D, et al. Molecular Cytogenetic Characterization of a New Wheat-rye 4R Chromosome Translocation Line Resistant to Powdery Mildew. Chromosome Res. 2013;21(4):419-32. PubMed PMID: 23836161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew. AU - An,Diaoguo, AU - Zheng,Qi, AU - Zhou,Yilin, AU - Ma,Pengtao, AU - Lv,Zhenling, AU - Li,Lihui, AU - Li,Bin, AU - Luo,Qiaoling, AU - Xu,Hongxing, AU - Xu,Yunfeng, Y1 - 2013/07/09/ PY - 2013/04/13/received PY - 2013/06/06/accepted PY - 2013/05/29/revised PY - 2013/7/10/entrez PY - 2013/7/10/pubmed PY - 2014/2/4/medline SP - 419 EP - 32 JF - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JO - Chromosome Res VL - 21 IS - 4 N2 - Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat-rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat-rye T4BL·4RL and T7AS·4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgt isolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7, Pm8, Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement. SN - 1573-6849 UR - https://www.unboundmedicine.com/medline/citation/23836161/Molecular_cytogenetic_characterization_of_a_new_wheat_rye_4R_chromosome_translocation_line_resistant_to_powdery_mildew_ L2 - https://doi.org/10.1007/s10577-013-9366-8 DB - PRIME DP - Unbound Medicine ER -