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Expression of ion transporters in gill mitochondrion-rich cells in Japanese eel acclimated to a wide range of environmental salinity.
Comp Biochem Physiol A Mol Integr Physiol. 2013 Oct; 166(2):323-32.CB

Abstract

We examined morphological changes and molecular mechanisms of ion regulation in mitochondrion-rich (MR) cells of Japanese eel acclimated to different environmental salinities. Electron microscopic observations revealed that the apical membrane of MR cells appeared as a flat or slightly projecting disk with a mesh-like structure on its surface in eel acclimated to freshwater (FW). In seawater (SW)-acclimated eel, in contrast, the apical membrane of MR cells showed a slightly concave surface without a mesh-like structure. The mRNA expression of Na(+)/H(+) exchanger-3 (NHE3) in deionized FW and normal SW was higher than that in normal FW and 30%-diluted SW. Expression of Na(+)/K(+)/2Cl(-) cotransporter-1a (NKCC1a) became higher with increasing environmental salinity. Immunofluorescence staining showed that the apical NHE3 immunoreaction was stronger in deionized FW and normal SW than in the other groups. Basolateral NKCC1 immunoreaction was most intense in normal SW. These results indicate that apical NHE3 is involved in ion uptake in fish acclimated to hypotonic environments, and that basolateral NKCC1 is important for acclimation to hypertonic environments. The relatively high expression of NHE3 in SW further indicates a possible role of NHE3 in acid-base regulation in the gills in SW-acclimated fish.

Authors+Show Affiliations

Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan. myseo@marine.fs.a.u-tokyo.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23838143

Citation

Seo, Mi Young, et al. "Expression of Ion Transporters in Gill Mitochondrion-rich Cells in Japanese Eel Acclimated to a Wide Range of Environmental Salinity." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 166, no. 2, 2013, pp. 323-32.
Seo MY, Mekuchi M, Teranishi K, et al. Expression of ion transporters in gill mitochondrion-rich cells in Japanese eel acclimated to a wide range of environmental salinity. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(2):323-32.
Seo, M. Y., Mekuchi, M., Teranishi, K., & Kaneko, T. (2013). Expression of ion transporters in gill mitochondrion-rich cells in Japanese eel acclimated to a wide range of environmental salinity. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 166(2), 323-32. https://doi.org/10.1016/j.cbpa.2013.07.004
Seo MY, et al. Expression of Ion Transporters in Gill Mitochondrion-rich Cells in Japanese Eel Acclimated to a Wide Range of Environmental Salinity. Comp Biochem Physiol A Mol Integr Physiol. 2013;166(2):323-32. PubMed PMID: 23838143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of ion transporters in gill mitochondrion-rich cells in Japanese eel acclimated to a wide range of environmental salinity. AU - Seo,Mi Young, AU - Mekuchi,Miyuki, AU - Teranishi,Keitaro, AU - Kaneko,Toyoji, Y1 - 2013/07/06/ PY - 2013/04/20/received PY - 2013/07/02/revised PY - 2013/07/02/accepted PY - 2013/7/11/entrez PY - 2013/7/11/pubmed PY - 2014/3/25/medline KW - Gill KW - Japanese eel KW - Mitochondrion-rich cell KW - NHE3 KW - NKCC1a KW - Osmoregulation KW - V-ATPase SP - 323 EP - 32 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 166 IS - 2 N2 - We examined morphological changes and molecular mechanisms of ion regulation in mitochondrion-rich (MR) cells of Japanese eel acclimated to different environmental salinities. Electron microscopic observations revealed that the apical membrane of MR cells appeared as a flat or slightly projecting disk with a mesh-like structure on its surface in eel acclimated to freshwater (FW). In seawater (SW)-acclimated eel, in contrast, the apical membrane of MR cells showed a slightly concave surface without a mesh-like structure. The mRNA expression of Na(+)/H(+) exchanger-3 (NHE3) in deionized FW and normal SW was higher than that in normal FW and 30%-diluted SW. Expression of Na(+)/K(+)/2Cl(-) cotransporter-1a (NKCC1a) became higher with increasing environmental salinity. Immunofluorescence staining showed that the apical NHE3 immunoreaction was stronger in deionized FW and normal SW than in the other groups. Basolateral NKCC1 immunoreaction was most intense in normal SW. These results indicate that apical NHE3 is involved in ion uptake in fish acclimated to hypotonic environments, and that basolateral NKCC1 is important for acclimation to hypertonic environments. The relatively high expression of NHE3 in SW further indicates a possible role of NHE3 in acid-base regulation in the gills in SW-acclimated fish. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/23838143/Expression_of_ion_transporters_in_gill_mitochondrion_rich_cells_in_Japanese_eel_acclimated_to_a_wide_range_of_environmental_salinity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(13)00185-2 DB - PRIME DP - Unbound Medicine ER -