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Characterisation of diarylheptanoid- and flavonoid-type phenolics in Corylus avellana L. leaves and bark by HPLC/DAD-ESI/MS.
Phytochem Anal. 2013 Sep-Oct; 24(5):493-503.PA

Abstract

INTRODUCTION

The leaves of Corylus avellana L. (common hazel, Betulaceae), a plant with a wide distribution in Europe, have been used in folk medicine for various diseases, but phytochemical exploration of C. avellana is still incomplete. To the best of our knowledge there is no previous report concerning diarylheptanoids in C. avellana, although these compounds show a frequent occurrence among Betulaceae plants.

OBJECTIVE

To improve existing online chromatographic methods for the investigation of the phenolic compounds in C. avellana leaves and bark, focusing on diarylheptanoid-type molecules.

METHODS

Dried and powdered leaves and bark of C. avellana were extracted with increasing polarity solvents (n-hexane, chloroform, ethyl acetate and methanol) in Soxhlet extractor apparatus. For the characterisation of the phenolic compounds in the ethyl acetate and methanolic extracts, UV spectral data, obtained by LC with a diode-array detector (DAD), accurate molecular mass and formula, acquired by LC and electrospray ionisation (ESI) with time-of-flight (TOF) MS and fragmentation pattern, given by LC-ESI/MS/MS analyses were used. Quantitation of the compounds was performed by LC-MS/MS.

RESULTS

In the methanolic and ethyl acetate extracts of C. avellana bark four flavonoid glycosides and a caffeoyl hexoside derivative were detected and characterised, while in C. avellana leaves, seven diarylheptanoid-type molecules were tentatively identified in addition to six flavonoid components. As far as we know this is the first study where the presence of diarylheptanoids in C. avellana is reported.

CONCLUSION

The improved HPLC/DAD-ESI/MS method was successfully utilised for the characterisation and quantitation of the phenolic compounds in C. avellana bark and leaves extracts.

Authors+Show Affiliations

Department of Pharmacognosy, Semmelweis University, H-1085, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

23839957

Citation

Riethmüller, Eszter, et al. "Characterisation of Diarylheptanoid- and Flavonoid-type Phenolics in Corylus Avellana L. Leaves and Bark By HPLC/DAD-ESI/MS." Phytochemical Analysis : PCA, vol. 24, no. 5, 2013, pp. 493-503.
Riethmüller E, Alberti A, Tóth G, et al. Characterisation of diarylheptanoid- and flavonoid-type phenolics in Corylus avellana L. leaves and bark by HPLC/DAD-ESI/MS. Phytochem Anal. 2013;24(5):493-503.
Riethmüller, E., Alberti, A., Tóth, G., Béni, S., Ortolano, F., & Kéry, A. (2013). Characterisation of diarylheptanoid- and flavonoid-type phenolics in Corylus avellana L. leaves and bark by HPLC/DAD-ESI/MS. Phytochemical Analysis : PCA, 24(5), 493-503. https://doi.org/10.1002/pca.2452
Riethmüller E, et al. Characterisation of Diarylheptanoid- and Flavonoid-type Phenolics in Corylus Avellana L. Leaves and Bark By HPLC/DAD-ESI/MS. Phytochem Anal. 2013 Sep-Oct;24(5):493-503. PubMed PMID: 23839957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation of diarylheptanoid- and flavonoid-type phenolics in Corylus avellana L. leaves and bark by HPLC/DAD-ESI/MS. AU - Riethmüller,Eszter, AU - Alberti,Agnes, AU - Tóth,Gergő, AU - Béni,Szabolcs, AU - Ortolano,Francesco, AU - Kéry,Agnes, Y1 - 2013/07/09/ PY - 2012/08/01/received PY - 2013/04/25/revised PY - 2013/04/29/accepted PY - 2013/7/11/entrez PY - 2013/7/11/pubmed PY - 2014/4/1/medline KW - Corylus avellana KW - HPLC-MS KW - diarylheptanoid KW - flavonoid KW - hazel SP - 493 EP - 503 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 24 IS - 5 N2 - INTRODUCTION: The leaves of Corylus avellana L. (common hazel, Betulaceae), a plant with a wide distribution in Europe, have been used in folk medicine for various diseases, but phytochemical exploration of C. avellana is still incomplete. To the best of our knowledge there is no previous report concerning diarylheptanoids in C. avellana, although these compounds show a frequent occurrence among Betulaceae plants. OBJECTIVE: To improve existing online chromatographic methods for the investigation of the phenolic compounds in C. avellana leaves and bark, focusing on diarylheptanoid-type molecules. METHODS: Dried and powdered leaves and bark of C. avellana were extracted with increasing polarity solvents (n-hexane, chloroform, ethyl acetate and methanol) in Soxhlet extractor apparatus. For the characterisation of the phenolic compounds in the ethyl acetate and methanolic extracts, UV spectral data, obtained by LC with a diode-array detector (DAD), accurate molecular mass and formula, acquired by LC and electrospray ionisation (ESI) with time-of-flight (TOF) MS and fragmentation pattern, given by LC-ESI/MS/MS analyses were used. Quantitation of the compounds was performed by LC-MS/MS. RESULTS: In the methanolic and ethyl acetate extracts of C. avellana bark four flavonoid glycosides and a caffeoyl hexoside derivative were detected and characterised, while in C. avellana leaves, seven diarylheptanoid-type molecules were tentatively identified in addition to six flavonoid components. As far as we know this is the first study where the presence of diarylheptanoids in C. avellana is reported. CONCLUSION: The improved HPLC/DAD-ESI/MS method was successfully utilised for the characterisation and quantitation of the phenolic compounds in C. avellana bark and leaves extracts. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/23839957/Characterisation_of_diarylheptanoid__and_flavonoid_type_phenolics_in_Corylus_avellana_L__leaves_and_bark_by_HPLC/DAD_ESI/MS_ L2 - https://doi.org/10.1002/pca.2452 DB - PRIME DP - Unbound Medicine ER -