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Analysis of multiple constituents in Cong-Ming-Tang, a Chinese herbal formula for the treatment of amnesia, by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
Phytochem Anal. 2013 Nov-Dec; 24(6):677-88.PA

Abstract

INTRODUCTION

Cong-Ming-Tang (CMT), named smart-soup in English, is a well-known traditional Chinese medicine formula for the treatment of amnesia in China. However, the isolation, purification and identification procedures of the major bioactive constituents in CMT are difficult and time consuming.

OBJECTIVE

To establish a rapid and sensitive high-performance liquid chromatography/electrospray ionisation with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF/MS/MS) method that could be applied to rapidly separate and identify the major bioactive constituents in CMT.

METHODS

Methanolic extract of CMT was used for HPLC-QTOF/MS/MS analysis. Separation was performed on an Agilent Poroshell 120 EC- C18 column (2.7 ×100 mm .i.d., 2.7 µm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Both positive and negative ion modes were employed.

RESULTS

This analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retention times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phenylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules.

CONCLUSION

HPLC-QTOF/MS/MS provides a new powerful approach to identify the major chemical constituents in CMT rapidly and accurately. This study proposed a series of potential bioactive components without preparative isolation from the crude extract of CMT and indicated that the HPLC-QTOF/MS/MS method also can be a promising tool for the analysis of other traditional Chinese medicines.

Authors+Show Affiliations

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23839964

Citation

Ling, Yun, et al. "Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, By High-performance Liquid Chromatography With Quadrupole Time-of-flight Mass Spectrometry." Phytochemical Analysis : PCA, vol. 24, no. 6, 2013, pp. 677-88.
Ling Y, Li Z, Chen M, et al. Analysis of multiple constituents in Cong-Ming-Tang, a Chinese herbal formula for the treatment of amnesia, by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Phytochem Anal. 2013;24(6):677-88.
Ling, Y., Li, Z., Chen, M., Sun, Z., Fan, M., & Huang, C. (2013). Analysis of multiple constituents in Cong-Ming-Tang, a Chinese herbal formula for the treatment of amnesia, by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Phytochemical Analysis : PCA, 24(6), 677-88. https://doi.org/10.1002/pca.2454
Ling Y, et al. Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, By High-performance Liquid Chromatography With Quadrupole Time-of-flight Mass Spectrometry. Phytochem Anal. 2013 Nov-Dec;24(6):677-88. PubMed PMID: 23839964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of multiple constituents in Cong-Ming-Tang, a Chinese herbal formula for the treatment of amnesia, by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. AU - Ling,Yun, AU - Li,Zhixiong, AU - Chen,Mingcang, AU - Sun,Zhaolin, AU - Fan,Mingsong, AU - Huang,Chenggang, Y1 - 2013/07/09/ PY - 2013/01/25/received PY - 2013/05/01/revised PY - 2013/05/02/accepted PY - 2013/7/11/entrez PY - 2013/7/11/pubmed PY - 2014/5/30/medline KW - Cong-Ming-Tang KW - HPLC-QTOF/MS/MS KW - chemical constituents KW - traditional Chinese medicine formula SP - 677 EP - 88 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 24 IS - 6 N2 - INTRODUCTION: Cong-Ming-Tang (CMT), named smart-soup in English, is a well-known traditional Chinese medicine formula for the treatment of amnesia in China. However, the isolation, purification and identification procedures of the major bioactive constituents in CMT are difficult and time consuming. OBJECTIVE: To establish a rapid and sensitive high-performance liquid chromatography/electrospray ionisation with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF/MS/MS) method that could be applied to rapidly separate and identify the major bioactive constituents in CMT. METHODS: Methanolic extract of CMT was used for HPLC-QTOF/MS/MS analysis. Separation was performed on an Agilent Poroshell 120 EC- C18 column (2.7 ×100 mm .i.d., 2.7 µm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Both positive and negative ion modes were employed. RESULTS: This analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retention times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phenylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules. CONCLUSION: HPLC-QTOF/MS/MS provides a new powerful approach to identify the major chemical constituents in CMT rapidly and accurately. This study proposed a series of potential bioactive components without preparative isolation from the crude extract of CMT and indicated that the HPLC-QTOF/MS/MS method also can be a promising tool for the analysis of other traditional Chinese medicines. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/23839964/Analysis_of_multiple_constituents_in_Cong_Ming_Tang_a_Chinese_herbal_formula_for_the_treatment_of_amnesia_by_high_performance_liquid_chromatography_with_quadrupole_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/pca.2454 DB - PRIME DP - Unbound Medicine ER -