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In vitro antioxidant and cytotoxic properties of ethanol extract of Alpinia oxyphylla fruits.
Pharm Biol. 2013 Nov; 51(11):1419-25.PB

Abstract

CONTEXT

Alpinia oxyphylla Miquel (Zingiberaceae) is a traditional Chinese herbal medicine widely used for the treatment of intestinal disorders, urosis and diuresis. However, information about antioxidant and cytotoxic properties of its fruits remains to be elucidated.

OBJECTIVE

The ethanol crude extract (CE) and its fractions [petroleum ether fraction (PF), ethyl acetate fraction (EF), n-butanol fraction (BF) and water fraction (WF) extracted by petroleum ether, ethyl acetate, n-butanol and water, respectively] of A. oxyphylla fruits were investigated for their antioxidant activity and cytotoxicity.

MATERIALS AND METHODS

The total phenolic content (TPC) and antioxidant activity of the extracts were determined by Folin-Ciocalteu reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), Trolox equivalent antioxidant capacity and reducing power assay. Cytotoxicity of the extracts (0-200 μg/mL) was tested on six human cancer cell lines (breast cancer cell line, cervix carcinoma cell line, lung adenocarcinoma cell line, liver carcinoma cell line, gastric cancer cell line and colon cancer cell line) using the sulforhodamine B assay.

RESULTS

The TPC of extracts varied from 8.2 to 20.3 mg gallic acid equivalents/g dry weight. DPPH radical scavenging effect of extracts decreased in the order of EF > BF > CE > PF > WF, with IC50 values ranging from 74.7 to 680.8 μg/mL. 2,2-azo-bis(3-Ethylbenzothiazoline-6-sulfoic acid) diammonium salt scavenging activity ranged from 0.118 to 0.236 mmol Trolox equivalence/mg extract. The extracts exhibited concentration-dependent reducing power, and EF showed the highest reducing ability. A satisfactory correlation (R(2) > 0.826) between TPC and antioxidant activity was observed. In addition, EF, PF and CE exhibited potent anticancer effects on six cancer cell lines with IC50 values ranging from 40.1 to 166.3 μg/mL.

DISCUSSION AND CONCLUSION

The ethanol extract of A. oxyphylla fruit, especially the EF, was found to possess potent antioxidant and anticancer activities, and thus a great potential for the application in food and drug products.

Authors+Show Affiliations

Shanghai Key Laboratory of Functional Materials Chemistry, and Research Center of Analysis and Test, East China University of Science and Technology , Shanghai , P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23841520

Citation

Wang, Cheng-zhong, et al. "In Vitro Antioxidant and Cytotoxic Properties of Ethanol Extract of Alpinia Oxyphylla Fruits." Pharmaceutical Biology, vol. 51, no. 11, 2013, pp. 1419-25.
Wang CZ, Yuan HH, Bao XL, et al. In vitro antioxidant and cytotoxic properties of ethanol extract of Alpinia oxyphylla fruits. Pharm Biol. 2013;51(11):1419-25.
Wang, C. Z., Yuan, H. H., Bao, X. L., & Lan, M. B. (2013). In vitro antioxidant and cytotoxic properties of ethanol extract of Alpinia oxyphylla fruits. Pharmaceutical Biology, 51(11), 1419-25. https://doi.org/10.3109/13880209.2013.794844
Wang CZ, et al. In Vitro Antioxidant and Cytotoxic Properties of Ethanol Extract of Alpinia Oxyphylla Fruits. Pharm Biol. 2013;51(11):1419-25. PubMed PMID: 23841520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant and cytotoxic properties of ethanol extract of Alpinia oxyphylla fruits. AU - Wang,Cheng-zhong, AU - Yuan,Hui-hui, AU - Bao,Xiao-li, AU - Lan,Min-bo, Y1 - 2013/07/10/ PY - 2013/7/12/entrez PY - 2013/7/12/pubmed PY - 2014/5/28/medline SP - 1419 EP - 25 JF - Pharmaceutical biology JO - Pharm Biol VL - 51 IS - 11 N2 - CONTEXT: Alpinia oxyphylla Miquel (Zingiberaceae) is a traditional Chinese herbal medicine widely used for the treatment of intestinal disorders, urosis and diuresis. However, information about antioxidant and cytotoxic properties of its fruits remains to be elucidated. OBJECTIVE: The ethanol crude extract (CE) and its fractions [petroleum ether fraction (PF), ethyl acetate fraction (EF), n-butanol fraction (BF) and water fraction (WF) extracted by petroleum ether, ethyl acetate, n-butanol and water, respectively] of A. oxyphylla fruits were investigated for their antioxidant activity and cytotoxicity. MATERIALS AND METHODS: The total phenolic content (TPC) and antioxidant activity of the extracts were determined by Folin-Ciocalteu reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), Trolox equivalent antioxidant capacity and reducing power assay. Cytotoxicity of the extracts (0-200 μg/mL) was tested on six human cancer cell lines (breast cancer cell line, cervix carcinoma cell line, lung adenocarcinoma cell line, liver carcinoma cell line, gastric cancer cell line and colon cancer cell line) using the sulforhodamine B assay. RESULTS: The TPC of extracts varied from 8.2 to 20.3 mg gallic acid equivalents/g dry weight. DPPH radical scavenging effect of extracts decreased in the order of EF > BF > CE > PF > WF, with IC50 values ranging from 74.7 to 680.8 μg/mL. 2,2-azo-bis(3-Ethylbenzothiazoline-6-sulfoic acid) diammonium salt scavenging activity ranged from 0.118 to 0.236 mmol Trolox equivalence/mg extract. The extracts exhibited concentration-dependent reducing power, and EF showed the highest reducing ability. A satisfactory correlation (R(2) > 0.826) between TPC and antioxidant activity was observed. In addition, EF, PF and CE exhibited potent anticancer effects on six cancer cell lines with IC50 values ranging from 40.1 to 166.3 μg/mL. DISCUSSION AND CONCLUSION: The ethanol extract of A. oxyphylla fruit, especially the EF, was found to possess potent antioxidant and anticancer activities, and thus a great potential for the application in food and drug products. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/23841520/In_vitro_antioxidant_and_cytotoxic_properties_of_ethanol_extract_of_Alpinia_oxyphylla_fruits_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2013.794844 DB - PRIME DP - Unbound Medicine ER -