Tags

Type your tag names separated by a space and hit enter

Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island.
Trans R Soc Trop Med Hyg. 2013 Aug; 107(8):493-501.TR

Abstract

BACKGROUND

The diagnostic accuracy of three faecal egg count techniques (Kato-Katz, McMaster and FLOTAC) to assess albendazole efficacy against soil-transmitted helminth (STH) infections was compared.

METHODS

The study is registered with Current Controlled Trials [identifier: ISRCTN90088840]. During September-November 2009, 304 school-aged children on Pemba Island, Tanzania, were screened and those infected with Ascaris lumbricoides, hookworm or Trichuris trichiura were treated with a single dose of albendazole (400 mg). Twenty-one days post-treatment, children provided a single stool sample which was examined using the same diagnostic methods. All stool samples were divided into two aliquots and one was fixed in 5% formalin and examined using FLOTAC and McMaster approximately 6 months after collection.

RESULTS

Using fresh stool samples, comparable prevalences were demonstrated for the three methods at baseline (90-92.2% for T. trichiura, 41.1-52.8% for hookworm, 32.9-37.2% for A. lumbricoides); FLOTAC was the most sensitive method at baseline and follow-up. Albendazole showed high cure rate (CR) against A. lumbricoides (90-97%), moderate CR against hookworm (63-72%) and very low CR against T. trichiura (6-9%), regardless of the technique used. Egg counts (eggs per gram) at baseline were similar for A. lumbricoides and for hookworm among the three methods, and higher using McMaster and Kato-Katz compared with FLOTAC for T. trichiura. All methods were similar for hookworm and A. lumbricoides egg reduction rate (ERR) estimation, but Kato-Katz indicated a significantly higher ERR than McMaster and FLOTAC for T. trichiura. Preserved stool samples revealed consistently lower FECs at baseline and follow-up for all STHs.

CONCLUSION

Further development and validation of standard protocols for anthelminthic drug efficacy evaluation must be pursued.

Authors+Show Affiliations

Department of Biology, University of Torino, 10122 Torino, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23843559

Citation

Albonico, Marco, et al. "Comparison of Three Copromicroscopic Methods to Assess Albendazole Efficacy Against Soil-transmitted Helminth Infections in School-aged Children On Pemba Island." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 107, no. 8, 2013, pp. 493-501.
Albonico M, Rinaldi L, Sciascia S, et al. Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island. Trans R Soc Trop Med Hyg. 2013;107(8):493-501.
Albonico, M., Rinaldi, L., Sciascia, S., Morgoglione, M. E., Piemonte, M., Maurelli, M. P., Musella, V., Utzinger, J., Ali, S. M., Ame, S. M., & Cringoli, G. (2013). Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island. Transactions of the Royal Society of Tropical Medicine and Hygiene, 107(8), 493-501. https://doi.org/10.1093/trstmh/trt051
Albonico M, et al. Comparison of Three Copromicroscopic Methods to Assess Albendazole Efficacy Against Soil-transmitted Helminth Infections in School-aged Children On Pemba Island. Trans R Soc Trop Med Hyg. 2013;107(8):493-501. PubMed PMID: 23843559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island. AU - Albonico,Marco, AU - Rinaldi,Laura, AU - Sciascia,Sonia, AU - Morgoglione,Maria E, AU - Piemonte,Monica, AU - Maurelli,Maria P, AU - Musella,Vincenzo, AU - Utzinger,Jürg, AU - Ali,Said M, AU - Ame,Shaali M, AU - Cringoli,Giuseppe, PY - 2013/7/12/entrez PY - 2013/7/12/pubmed PY - 2014/6/6/medline KW - Diagnostic accuracy KW - Drug efficacy KW - FLOTAC KW - Kato–Katz KW - McMaster KW - Soil-transmitted helminths SP - 493 EP - 501 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans. R. Soc. Trop. Med. Hyg. VL - 107 IS - 8 N2 - BACKGROUND: The diagnostic accuracy of three faecal egg count techniques (Kato-Katz, McMaster and FLOTAC) to assess albendazole efficacy against soil-transmitted helminth (STH) infections was compared. METHODS: The study is registered with Current Controlled Trials [identifier: ISRCTN90088840]. During September-November 2009, 304 school-aged children on Pemba Island, Tanzania, were screened and those infected with Ascaris lumbricoides, hookworm or Trichuris trichiura were treated with a single dose of albendazole (400 mg). Twenty-one days post-treatment, children provided a single stool sample which was examined using the same diagnostic methods. All stool samples were divided into two aliquots and one was fixed in 5% formalin and examined using FLOTAC and McMaster approximately 6 months after collection. RESULTS: Using fresh stool samples, comparable prevalences were demonstrated for the three methods at baseline (90-92.2% for T. trichiura, 41.1-52.8% for hookworm, 32.9-37.2% for A. lumbricoides); FLOTAC was the most sensitive method at baseline and follow-up. Albendazole showed high cure rate (CR) against A. lumbricoides (90-97%), moderate CR against hookworm (63-72%) and very low CR against T. trichiura (6-9%), regardless of the technique used. Egg counts (eggs per gram) at baseline were similar for A. lumbricoides and for hookworm among the three methods, and higher using McMaster and Kato-Katz compared with FLOTAC for T. trichiura. All methods were similar for hookworm and A. lumbricoides egg reduction rate (ERR) estimation, but Kato-Katz indicated a significantly higher ERR than McMaster and FLOTAC for T. trichiura. Preserved stool samples revealed consistently lower FECs at baseline and follow-up for all STHs. CONCLUSION: Further development and validation of standard protocols for anthelminthic drug efficacy evaluation must be pursued. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/23843559/Comparison_of_three_copromicroscopic_methods_to_assess_albendazole_efficacy_against_soil_transmitted_helminth_infections_in_school_aged_children_on_Pemba_Island_ L2 - https://academic.oup.com/trstmh/article-lookup/doi/10.1093/trstmh/trt051 DB - PRIME DP - Unbound Medicine ER -