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Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation.
Toxicol Appl Pharmacol. 2013 Oct 15; 272(2):313-24.TA

Abstract

Gossypetin, a flavone originally isolated from Hibiscus species, has been shown to possess antioxidant, antimicrobial, and antimutagenic activities. Here, we investigated the mechanism(s) underlying the anti-atherosclerotic potential of gossypetin. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity assay showed that the addition of >50μM of gossypetin could scavenge over 50% of DPPH radicals. The inhibitory effects of gossypetin on the lipid and protein oxidation of LDL were defined by thiobarbituric acid reactive substance (TBARS) assay, the relative electrophoretic mobility (REM) of oxidized LDL (ox-LDL), and fragmentation of apoB in the Cu(2+)-induced oxidation of LDL. Gossypetin showed potential in reducing ox-LDL-induced foam cell formation and intracellular lipid accumulation, and uptake ability of macrophages under non-cytotoxic concentrations. Molecular data showed that these influences of gossypetin might be mediated via peroxisome proliferator-activated receptor α (PPARα)/liver-X receptor α (LXRα)/ATP-binding cassette transporter A1 (ABCA1) and PPARγ/scavenger receptor CD36 pathways, as demonstrated by the transfection of PPARα siRNA or PPARγ expression vector. Our data implied that gossypetin regulated the PPAR signals, which in turn led to stimulation of cholesterol removal from macrophages and delay atherosclerosis. These results suggested that gossypetin potentially could be developed as an anti-atherosclerotic agent.

Authors+Show Affiliations

School of Nutrition, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23845592

Citation

Chen, Jing-Hsien, et al. "Anti-atherosclerotic Potential of Gossypetin Via Inhibiting LDL Oxidation and Foam Cell Formation." Toxicology and Applied Pharmacology, vol. 272, no. 2, 2013, pp. 313-24.
Chen JH, Tsai CW, Wang CP, et al. Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. Toxicol Appl Pharmacol. 2013;272(2):313-24.
Chen, J. H., Tsai, C. W., Wang, C. P., & Lin, H. H. (2013). Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. Toxicology and Applied Pharmacology, 272(2), 313-24. https://doi.org/10.1016/j.taap.2013.06.027
Chen JH, et al. Anti-atherosclerotic Potential of Gossypetin Via Inhibiting LDL Oxidation and Foam Cell Formation. Toxicol Appl Pharmacol. 2013 Oct 15;272(2):313-24. PubMed PMID: 23845592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. AU - Chen,Jing-Hsien, AU - Tsai,Chia-Wen, AU - Wang,Chi-Ping, AU - Lin,Hui-Hsuan, Y1 - 2013/07/08/ PY - 2013/02/26/received PY - 2013/06/26/revised PY - 2013/06/28/accepted PY - 2013/7/13/entrez PY - 2013/7/13/pubmed PY - 2013/12/16/medline KW - 1,1-Diphenyl-2-picrylhydrazyl KW - 3-(4,5-Dimethylthiazol-zyl)-2,5-diphenyltetrazolium bromide KW - ABCA1 KW - ATP-binding cassette transporter A1 KW - Atherosclerosis KW - Cholesterol removal KW - DPPH KW - ECL KW - Foam cells KW - Gossypetin KW - LDL KW - LXRα KW - MTT KW - Oxidation KW - Oxidized low-density lipoprotein KW - PPARs KW - REM KW - SDS-PAGE KW - SR-As KW - TBARS KW - TBS KW - Tris-buffered saline KW - acLDL KW - acetylated LDL KW - class A scavenge receptors KW - enhanced chemiluminescence KW - liver-X receptor α KW - low-density lipoprotein KW - ox-LDL KW - oxidized LDL KW - peroxisome proliferator-activated receptors KW - relative electrophoretic mobility KW - sodium dodecyl sulfate polyacrylamide gel electrophoresis KW - thiobarbituric acid reactive substances SP - 313 EP - 24 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 272 IS - 2 N2 - Gossypetin, a flavone originally isolated from Hibiscus species, has been shown to possess antioxidant, antimicrobial, and antimutagenic activities. Here, we investigated the mechanism(s) underlying the anti-atherosclerotic potential of gossypetin. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity assay showed that the addition of >50μM of gossypetin could scavenge over 50% of DPPH radicals. The inhibitory effects of gossypetin on the lipid and protein oxidation of LDL were defined by thiobarbituric acid reactive substance (TBARS) assay, the relative electrophoretic mobility (REM) of oxidized LDL (ox-LDL), and fragmentation of apoB in the Cu(2+)-induced oxidation of LDL. Gossypetin showed potential in reducing ox-LDL-induced foam cell formation and intracellular lipid accumulation, and uptake ability of macrophages under non-cytotoxic concentrations. Molecular data showed that these influences of gossypetin might be mediated via peroxisome proliferator-activated receptor α (PPARα)/liver-X receptor α (LXRα)/ATP-binding cassette transporter A1 (ABCA1) and PPARγ/scavenger receptor CD36 pathways, as demonstrated by the transfection of PPARα siRNA or PPARγ expression vector. Our data implied that gossypetin regulated the PPAR signals, which in turn led to stimulation of cholesterol removal from macrophages and delay atherosclerosis. These results suggested that gossypetin potentially could be developed as an anti-atherosclerotic agent. SN - 1096-0333 UR - https://www.unboundmedicine.com/medline/citation/23845592/Anti_atherosclerotic_potential_of_gossypetin_via_inhibiting_LDL_oxidation_and_foam_cell_formation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(13)00300-1 DB - PRIME DP - Unbound Medicine ER -