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Density dynamics of diverse Spiroplasma strains naturally infecting different species of Drosophila.
Fly (Austin). 2013 Jul-Sep; 7(3):204-10.F

Abstract

Facultative heritable bacterial endosymbionts can have dramatic effects on their hosts, ranging from mutualistic to parasitic. Within-host bacterial endosymbiont density plays a critical role in maintenance of a symbiotic relationship, as it can affect levels of vertical transmission and expression of phenotypic effects, both of which influence the infection prevalence in host populations. Species of genus Drosophila are infected with Spiroplasma, whose characterized phenotypic effects range from that of a male-killing reproductive parasite to beneficial defensive endosymbiont. For many strains of Spiroplasma infecting at least 17 species of Drosophila, however, the phenotypic effects are obscure. The infection prevalence of these Spiroplasma vary within and among Drosophila species, and little is known about the within-host density dynamics of these diverse strains. To characterize the patterns of Spiroplasma density variation among Drosophila we used quantitative PCR to assess bacterial titer at various life stages of three species of Drosophila naturally-infected with two different types of Spiroplasma. For naturally infected Drosophila species we found that non-male-killing infections had consistently lower densities than the male-killing infection. The patterns of Spiroplasma titer change during aging varied among Drosophila species infected with different Spiroplasma strains. Bacterial density varied within and among populations of Drosophila, with individuals from the population with the highest prevalence of infection having the highest density. This density variation underscores the complex interaction of Spiroplasma strain and host genetic background in determining endosymbiont density.

Authors+Show Affiliations

Division of Biological Sciences; University of California, San Diego; La Jolla, CA USA; Current affiliation: Department of Biology, University of Rochester, Rochester, NY USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23846301

Citation

Haselkorn, Tamara S., et al. "Density Dynamics of Diverse Spiroplasma Strains Naturally Infecting Different Species of Drosophila." Fly, vol. 7, no. 3, 2013, pp. 204-10.
Haselkorn TS, Watts TD, Markow TA. Density dynamics of diverse Spiroplasma strains naturally infecting different species of Drosophila. Fly (Austin). 2013;7(3):204-10.
Haselkorn, T. S., Watts, T. D., & Markow, T. A. (2013). Density dynamics of diverse Spiroplasma strains naturally infecting different species of Drosophila. Fly, 7(3), 204-10. https://doi.org/10.4161/fly.25469
Haselkorn TS, Watts TD, Markow TA. Density Dynamics of Diverse Spiroplasma Strains Naturally Infecting Different Species of Drosophila. Fly (Austin). 2013 Jul-Sep;7(3):204-10. PubMed PMID: 23846301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Density dynamics of diverse Spiroplasma strains naturally infecting different species of Drosophila. AU - Haselkorn,Tamara S, AU - Watts,Thomas D, AU - Markow,Therese A, Y1 - 2013/07/11/ PY - 2013/7/13/entrez PY - 2013/7/13/pubmed PY - 2014/5/31/medline KW - Drosophila KW - Spiroplasma KW - endosymbiont density KW - vertically-transmitted endosymbiont SP - 204 EP - 10 JF - Fly JO - Fly (Austin) VL - 7 IS - 3 N2 - Facultative heritable bacterial endosymbionts can have dramatic effects on their hosts, ranging from mutualistic to parasitic. Within-host bacterial endosymbiont density plays a critical role in maintenance of a symbiotic relationship, as it can affect levels of vertical transmission and expression of phenotypic effects, both of which influence the infection prevalence in host populations. Species of genus Drosophila are infected with Spiroplasma, whose characterized phenotypic effects range from that of a male-killing reproductive parasite to beneficial defensive endosymbiont. For many strains of Spiroplasma infecting at least 17 species of Drosophila, however, the phenotypic effects are obscure. The infection prevalence of these Spiroplasma vary within and among Drosophila species, and little is known about the within-host density dynamics of these diverse strains. To characterize the patterns of Spiroplasma density variation among Drosophila we used quantitative PCR to assess bacterial titer at various life stages of three species of Drosophila naturally-infected with two different types of Spiroplasma. For naturally infected Drosophila species we found that non-male-killing infections had consistently lower densities than the male-killing infection. The patterns of Spiroplasma titer change during aging varied among Drosophila species infected with different Spiroplasma strains. Bacterial density varied within and among populations of Drosophila, with individuals from the population with the highest prevalence of infection having the highest density. This density variation underscores the complex interaction of Spiroplasma strain and host genetic background in determining endosymbiont density. SN - 1933-6942 UR - https://www.unboundmedicine.com/medline/citation/23846301/Density_dynamics_of_diverse_Spiroplasma_strains_naturally_infecting_different_species_of_Drosophila_ L2 - https://www.tandfonline.com/doi/full/10.4161/fly.25469 DB - PRIME DP - Unbound Medicine ER -