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Simultaneous RP-HPLC-DAD separation, and determination of flavonoids and phenolic acids in Plantago L. species.
Chem Biodivers. 2013 Jul; 10(7):1305-16.CB

Abstract

A rapid reversed-phase (RP) high-performance liquid chromatography method was developed and applied for simultaneous separation, and determination of flavonoids and phenolic acids in eight Plantago L. taxa (P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. ssp. depauperata Pilger, P. holosteum ssp. holosteum, P. holosteum ssp. scopulorum (Degen) Horvatić, P. lagopus L., and P. maritima L.) growing in Croatia. Chromatographic separation was carried out on Zorbax Eclipse XDB-C18 using gradient elution with a H2 O (pH 2.5, adjusted with CF3 COOH) and MeCN mixture at 30°. The contents of analyzed phenolic compounds (% of the dry weight of the leaves, dw) varied among examined species: rutin (max. 0.024%, P. argentea), hyperoside (max. 0.020%, P. lagopus), quercitrin (max. 0.013%, P. holosteum ssp. holosteum), quercetin (max. 0.028%, P. holosteum ssp. scopulorum), chlorogenic acid (max. 0.115%, P. lagopus), and caffeic acid (max. 0.046%, P. coronopus). Isoquercitrin was detected only in P. argentea (0.020%), while isochlorogenic acid content was below limit of quantification in all investigated species. Multivariate analyses (UPGMA and PCA) showed significant differences in contents of investigated polyphenolic compounds between different Plantago taxa. Accordingly, investigated substances might be employed as chemotaxonomic markers in the study of the complex genus Plantago.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jurišić Grubešić R
Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb. rjurisic@pharma.hr
Srečnik G
No affiliation info available
Kremer D
No affiliation info available
Vuković Rodríguez J
No affiliation info available
Nikolić T
No affiliation info available
Vladimir-Knežević S
No affiliation info available

MeSH

Chromatography, High Pressure LiquidChromatography, Reverse-PhaseFlavonoidsHydroxybenzoatesMultivariate AnalysisPlant LeavesPlantagoPrincipal Component Analysis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23847075

Citation

Jurišić Grubešić, Renata, et al. "Simultaneous RP-HPLC-DAD Separation, and Determination of Flavonoids and Phenolic Acids in Plantago L. Species." Chemistry & Biodiversity, vol. 10, no. 7, 2013, pp. 1305-16.
Jurišić Grubešić R, Srečnik G, Kremer D, et al. Simultaneous RP-HPLC-DAD separation, and determination of flavonoids and phenolic acids in Plantago L. species. Chem Biodivers. 2013;10(7):1305-16.
Jurišić Grubešić, R., Srečnik, G., Kremer, D., Vuković Rodríguez, J., Nikolić, T., & Vladimir-Knežević, S. (2013). Simultaneous RP-HPLC-DAD separation, and determination of flavonoids and phenolic acids in Plantago L. species. Chemistry & Biodiversity, 10(7), 1305-16. https://doi.org/10.1002/cbdv.201200210
Jurišić Grubešić R, et al. Simultaneous RP-HPLC-DAD Separation, and Determination of Flavonoids and Phenolic Acids in Plantago L. Species. Chem Biodivers. 2013;10(7):1305-16. PubMed PMID: 23847075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous RP-HPLC-DAD separation, and determination of flavonoids and phenolic acids in Plantago L. species. AU - Jurišić Grubešić,Renata, AU - Srečnik,Goran, AU - Kremer,Dario, AU - Vuković Rodríguez,Jadranka, AU - Nikolić,Toni, AU - Vladimir-Knežević,Sanda, PY - 2012/06/18/received PY - 2013/7/13/entrez PY - 2013/7/13/pubmed PY - 2014/2/12/medline KW - Chemotaxonomy KW - Flavonoids KW - Phenolic acids KW - Plantago species SP - 1305 EP - 16 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 10 IS - 7 N2 - A rapid reversed-phase (RP) high-performance liquid chromatography method was developed and applied for simultaneous separation, and determination of flavonoids and phenolic acids in eight Plantago L. taxa (P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. ssp. depauperata Pilger, P. holosteum ssp. holosteum, P. holosteum ssp. scopulorum (Degen) Horvatić, P. lagopus L., and P. maritima L.) growing in Croatia. Chromatographic separation was carried out on Zorbax Eclipse XDB-C18 using gradient elution with a H2 O (pH 2.5, adjusted with CF3 COOH) and MeCN mixture at 30°. The contents of analyzed phenolic compounds (% of the dry weight of the leaves, dw) varied among examined species: rutin (max. 0.024%, P. argentea), hyperoside (max. 0.020%, P. lagopus), quercitrin (max. 0.013%, P. holosteum ssp. holosteum), quercetin (max. 0.028%, P. holosteum ssp. scopulorum), chlorogenic acid (max. 0.115%, P. lagopus), and caffeic acid (max. 0.046%, P. coronopus). Isoquercitrin was detected only in P. argentea (0.020%), while isochlorogenic acid content was below limit of quantification in all investigated species. Multivariate analyses (UPGMA and PCA) showed significant differences in contents of investigated polyphenolic compounds between different Plantago taxa. Accordingly, investigated substances might be employed as chemotaxonomic markers in the study of the complex genus Plantago. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/23847075/Simultaneous_RP_HPLC_DAD_separation_and_determination_of_flavonoids_and_phenolic_acids_in_Plantago_L__species_ L2 - https://doi.org/10.1002/cbdv.201200210 DB - PRIME DP - Unbound Medicine ER -