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Regulation of lipid production by acetylcholine signalling in human sebaceous glands.
J Dermatol Sci. 2013 Nov; 72(2):116-22.JD

Abstract

BACKGROUND

The extraneuronal cholinergic system has been implicated in numerous functions in the skin, such as terminal differentiation, barrier formation, sweat secretion and the microcirculation. However, the evidence for cholinergic signalling in sebaceous glands is lacking, and its role needs to be clarified.

OBJECTIVE

We investigated the role of acetylcholine signalling in sebaceous glands using human sebocytes and a clinical study using botulinum toxin.

METHODS

Immunohistochemistry and immunocytofluorescence were performed to evaluate cholinergic receptor levels in sebaceous glands. Lipid levels were assessed by Oil Red O staining and signalling pathways by Western blotting. To evaluate the clinical relevance, we also assessed the effect of botulinum toxin on sebum production in healthy volunteers.

RESULTS

We demonstrated that human skin sebaceous glands in vivo and sebocytes in vitro express nicotinic acetylcholine receptor α7 (nAchRα7), and that acetylcholine increased lipid synthesis in a dose-dependent manner. When sebocytes were incubated with α-bungarotoxin, a competitive nAchR antagonist, acetylcholine failed to up-regulate lipid synthesis. Twenty healthy volunteers were enrolled in a double-blind, placebo-controlled, split-face study. A marked decrease in sebum production on the botulinum-treated side was found in volunteers with oily skin.

CONCLUSION

These results provide evidence that acetylcholine signalling plays a significant role in human sebaceous gland biology and identify acetylcholine signalling as a promising target in the clinical management of disorders in which sebum production is increased, such as acne vulgaris.

Authors+Show Affiliations

Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23849311

Citation

Li, Zheng Jun, et al. "Regulation of Lipid Production By Acetylcholine Signalling in Human Sebaceous Glands." Journal of Dermatological Science, vol. 72, no. 2, 2013, pp. 116-22.
Li ZJ, Park SB, Sohn KC, et al. Regulation of lipid production by acetylcholine signalling in human sebaceous glands. J Dermatol Sci. 2013;72(2):116-22.
Li, Z. J., Park, S. B., Sohn, K. C., Lee, Y., Seo, Y. J., Kim, C. D., Kim, Y. S., Lee, J. H., & Im, M. (2013). Regulation of lipid production by acetylcholine signalling in human sebaceous glands. Journal of Dermatological Science, 72(2), 116-22. https://doi.org/10.1016/j.jdermsci.2013.06.009
Li ZJ, et al. Regulation of Lipid Production By Acetylcholine Signalling in Human Sebaceous Glands. J Dermatol Sci. 2013;72(2):116-22. PubMed PMID: 23849311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of lipid production by acetylcholine signalling in human sebaceous glands. AU - Li,Zheng Jun, AU - Park,Seung Bae, AU - Sohn,Kyung Cheol, AU - Lee,Young, AU - Seo,Young Joon, AU - Kim,Chang Deok, AU - Kim,Youn Sung, AU - Lee,Jeung Hoon, AU - Im,Myung, Y1 - 2013/06/28/ PY - 2013/03/19/received PY - 2013/06/04/revised PY - 2013/06/16/accepted PY - 2013/7/16/entrez PY - 2013/7/16/pubmed PY - 2014/5/29/medline KW - Acetylcholine KW - Botulinum toxin KW - Lipid KW - Sebaceous gland KW - Sebocyte SP - 116 EP - 22 JF - Journal of dermatological science JO - J. Dermatol. Sci. VL - 72 IS - 2 N2 - BACKGROUND: The extraneuronal cholinergic system has been implicated in numerous functions in the skin, such as terminal differentiation, barrier formation, sweat secretion and the microcirculation. However, the evidence for cholinergic signalling in sebaceous glands is lacking, and its role needs to be clarified. OBJECTIVE: We investigated the role of acetylcholine signalling in sebaceous glands using human sebocytes and a clinical study using botulinum toxin. METHODS: Immunohistochemistry and immunocytofluorescence were performed to evaluate cholinergic receptor levels in sebaceous glands. Lipid levels were assessed by Oil Red O staining and signalling pathways by Western blotting. To evaluate the clinical relevance, we also assessed the effect of botulinum toxin on sebum production in healthy volunteers. RESULTS: We demonstrated that human skin sebaceous glands in vivo and sebocytes in vitro express nicotinic acetylcholine receptor α7 (nAchRα7), and that acetylcholine increased lipid synthesis in a dose-dependent manner. When sebocytes were incubated with α-bungarotoxin, a competitive nAchR antagonist, acetylcholine failed to up-regulate lipid synthesis. Twenty healthy volunteers were enrolled in a double-blind, placebo-controlled, split-face study. A marked decrease in sebum production on the botulinum-treated side was found in volunteers with oily skin. CONCLUSION: These results provide evidence that acetylcholine signalling plays a significant role in human sebaceous gland biology and identify acetylcholine signalling as a promising target in the clinical management of disorders in which sebum production is increased, such as acne vulgaris. SN - 1873-569X UR - https://www.unboundmedicine.com/medline/citation/23849311/Regulation_of_lipid_production_by_acetylcholine_signalling_in_human_sebaceous_glands_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-1811(13)00219-3 DB - PRIME DP - Unbound Medicine ER -