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Evaluation of hydro-alcoholic extract of Eclipta alba for its multidrug resistance reversal potential: an in vitro study.
Nutr Cancer. 2013; 65(5):775-80.NC

Abstract

The development of multidrug resistance (MDR) causes problems in the chemotherapy of human cancer. The present study was designed to evaluate and establish the role of Eclipta alba as MDR reversal agent using multidrug resistant hepatocellular carcinoma cell line (DR-HepG2). To develop DR-HepG2, hepatocellular carcinoma cell line (HepG2) was transfected with 2-Acetylaminofluorene (AAF) and Aflatoxin B1 (AFB). Cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) and standard anti-ancer drug Doxorubicin (DOX) were determined in DR-HepG2 and the parental cells HepG2 using MTT assay. The expression level of MDR1 gene and P-glycoprotein (P-gp) level was analyzed by RT-PCR and western blotting. From the present investigation, it was found that EAE (10 and 20 μg/ml) could significantly inhibit cell proliferation in DR-HepG2 whereas DOX (0.5 μg/ml) could not because of enhancement effect of MDR1/P-gp. This study demonstrated for the first time the antiproliferative activities of EAE in multidrug resistant DR-HepG2 cells. The findings revealed that Eclipta alba components are effective inhibitors of MDR1/P-gp.

Authors+Show Affiliations

Institute of Science, Nirma University, Chharodi, Ahmedabad, Gujarat, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23859045

Citation

Chaudhary, Harshita, et al. "Evaluation of Hydro-alcoholic Extract of Eclipta Alba for Its Multidrug Resistance Reversal Potential: an in Vitro Study." Nutrition and Cancer, vol. 65, no. 5, 2013, pp. 775-80.
Chaudhary H, Jena PK, Seshadri S. Evaluation of hydro-alcoholic extract of Eclipta alba for its multidrug resistance reversal potential: an in vitro study. Nutr Cancer. 2013;65(5):775-80.
Chaudhary, H., Jena, P. K., & Seshadri, S. (2013). Evaluation of hydro-alcoholic extract of Eclipta alba for its multidrug resistance reversal potential: an in vitro study. Nutrition and Cancer, 65(5), 775-80. https://doi.org/10.1080/01635581.2013.789116
Chaudhary H, Jena PK, Seshadri S. Evaluation of Hydro-alcoholic Extract of Eclipta Alba for Its Multidrug Resistance Reversal Potential: an in Vitro Study. Nutr Cancer. 2013;65(5):775-80. PubMed PMID: 23859045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of hydro-alcoholic extract of Eclipta alba for its multidrug resistance reversal potential: an in vitro study. AU - Chaudhary,Harshita, AU - Jena,Prasant Kumar, AU - Seshadri,Sriram, PY - 2013/7/18/entrez PY - 2013/7/19/pubmed PY - 2014/2/12/medline SP - 775 EP - 80 JF - Nutrition and cancer JO - Nutr Cancer VL - 65 IS - 5 N2 - The development of multidrug resistance (MDR) causes problems in the chemotherapy of human cancer. The present study was designed to evaluate and establish the role of Eclipta alba as MDR reversal agent using multidrug resistant hepatocellular carcinoma cell line (DR-HepG2). To develop DR-HepG2, hepatocellular carcinoma cell line (HepG2) was transfected with 2-Acetylaminofluorene (AAF) and Aflatoxin B1 (AFB). Cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) and standard anti-ancer drug Doxorubicin (DOX) were determined in DR-HepG2 and the parental cells HepG2 using MTT assay. The expression level of MDR1 gene and P-glycoprotein (P-gp) level was analyzed by RT-PCR and western blotting. From the present investigation, it was found that EAE (10 and 20 μg/ml) could significantly inhibit cell proliferation in DR-HepG2 whereas DOX (0.5 μg/ml) could not because of enhancement effect of MDR1/P-gp. This study demonstrated for the first time the antiproliferative activities of EAE in multidrug resistant DR-HepG2 cells. The findings revealed that Eclipta alba components are effective inhibitors of MDR1/P-gp. SN - 1532-7914 UR - https://www.unboundmedicine.com/medline/citation/23859045/Evaluation_of_hydro_alcoholic_extract_of_Eclipta_alba_for_its_multidrug_resistance_reversal_potential:_an_in_vitro_study_ L2 - https://www.tandfonline.com/doi/full/10.1080/01635581.2013.789116 DB - PRIME DP - Unbound Medicine ER -