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Serum microRNA-21 and microRNA-221 as potential biomarkers for cerebrovascular disease.
J Vasc Res. 2013; 50(4):346-54.JV

Abstract

BACKGROUND/AIMS

MicroRNA miR-21, miR-221 and miR-145 have been implicated in the cardiovascular system. We aimed to compare the serum levels of the three microRNAs (miRNAs) in different severities of cerebrovascular diseases and evaluate the feasibility of using these miRNAs as biomarkers for stroke.

METHODS

We enrolled 167 subjects with ischemic stroke, 66 atherosclerosis subjects with any carotid plaque score and 157 healthy controls. These three types of subjects represent three levels of severity in cerebrovascular diseases. Analysis of covariance was used to evaluate the relationship between miRNAs and disease severity with adjustment for conventional risk factors. To test the prediction for stroke, we built regression models containing the serum miRNA levels and risk factors. Prediction capabilities were compared by the receiver operating characteristic curves.

RESULTS

Stroke patients and atherosclerosis subjects had significantly higher miR-21 and lower miR-221 serum levels than healthy controls, while the miR-145 expression was too low to provide useful information in this regard. The best model showed that miR-21 and miR-221 were independent predictors. There was a 6.2-fold increase for stroke risk when miR-21 levels increase by log₁₀2(-ΔCt) = 1, while a 10.4-fold increase was observed as miR-221 decreases by log₁₀2(-ΔCt) = 1.

CONCLUSIONS

Serum miR-145 was not detected in over 50% of the patients and it may not be an ideal marker to predict stroke. MiR-21 and miR-221 are novel biomarkers for atherosclerosis and stroke.

Authors+Show Affiliations

Department of Medical Research, Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23860376

Citation

Tsai, Pei-Chien, et al. "Serum microRNA-21 and microRNA-221 as Potential Biomarkers for Cerebrovascular Disease." Journal of Vascular Research, vol. 50, no. 4, 2013, pp. 346-54.
Tsai PC, Liao YC, Wang YS, et al. Serum microRNA-21 and microRNA-221 as potential biomarkers for cerebrovascular disease. J Vasc Res. 2013;50(4):346-54.
Tsai, P. C., Liao, Y. C., Wang, Y. S., Lin, H. F., Lin, R. T., & Juo, S. H. (2013). Serum microRNA-21 and microRNA-221 as potential biomarkers for cerebrovascular disease. Journal of Vascular Research, 50(4), 346-54. https://doi.org/10.1159/000351767
Tsai PC, et al. Serum microRNA-21 and microRNA-221 as Potential Biomarkers for Cerebrovascular Disease. J Vasc Res. 2013;50(4):346-54. PubMed PMID: 23860376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum microRNA-21 and microRNA-221 as potential biomarkers for cerebrovascular disease. AU - Tsai,Pei-Chien, AU - Liao,Yi-Chu, AU - Wang,Yung-Song, AU - Lin,Hsiu-Fen, AU - Lin,Ruey-Tay, AU - Juo,Suh-Hang Hank, Y1 - 2013/07/13/ PY - 2013/01/16/received PY - 2013/04/21/accepted PY - 2013/7/18/entrez PY - 2013/7/19/pubmed PY - 2013/10/29/medline SP - 346 EP - 54 JF - Journal of vascular research JO - J Vasc Res VL - 50 IS - 4 N2 - BACKGROUND/AIMS: MicroRNA miR-21, miR-221 and miR-145 have been implicated in the cardiovascular system. We aimed to compare the serum levels of the three microRNAs (miRNAs) in different severities of cerebrovascular diseases and evaluate the feasibility of using these miRNAs as biomarkers for stroke. METHODS: We enrolled 167 subjects with ischemic stroke, 66 atherosclerosis subjects with any carotid plaque score and 157 healthy controls. These three types of subjects represent three levels of severity in cerebrovascular diseases. Analysis of covariance was used to evaluate the relationship between miRNAs and disease severity with adjustment for conventional risk factors. To test the prediction for stroke, we built regression models containing the serum miRNA levels and risk factors. Prediction capabilities were compared by the receiver operating characteristic curves. RESULTS: Stroke patients and atherosclerosis subjects had significantly higher miR-21 and lower miR-221 serum levels than healthy controls, while the miR-145 expression was too low to provide useful information in this regard. The best model showed that miR-21 and miR-221 were independent predictors. There was a 6.2-fold increase for stroke risk when miR-21 levels increase by log₁₀2(-ΔCt) = 1, while a 10.4-fold increase was observed as miR-221 decreases by log₁₀2(-ΔCt) = 1. CONCLUSIONS: Serum miR-145 was not detected in over 50% of the patients and it may not be an ideal marker to predict stroke. MiR-21 and miR-221 are novel biomarkers for atherosclerosis and stroke. SN - 1423-0135 UR - https://www.unboundmedicine.com/medline/citation/23860376/Serum_microRNA_21_and_microRNA_221_as_potential_biomarkers_for_cerebrovascular_disease_ L2 - https://www.karger.com?DOI=10.1159/000351767 DB - PRIME DP - Unbound Medicine ER -