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Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma.
Med Sci Monit. 2013 Jul 17; 19:584-91.MS

Abstract

BACKGROUND

The aim of the study was to evaluate the presence of cagA gene Helicobacter pylori in etiopathogenesis of initiation and development of larynx squamous cell carcinoma (LSCC) and its predictable role as a prognostic factor.

MATERIAL AND METHODS

The prospective, controlled study involved a series of 75 patients (65 male, 10 female, mean age 59.1 years, range 43 to 79 years) with larynx cancer. Samples of larynx cancerous tissue, each of 10-15 mg, were obtained from fresh tissues and were used for nucleic acid purification. DNA was extracted from 225 samples (larynx tumor - I (75), margin of tumor and normal tissue - II (75) and normal larynx tissue from opposite side to the tumor - III). All samples were subjected to H. pylori ureA detection by the PCR H. pylori diagnostic test. Samples that were positive for ureA H. pylori gene were evaluated for cagA H. pylori gene.

RESULTS

Presence of H. pylori cagA gene was identified in 46,7% to 49,3% of 75 H. pylori ureA gene-positive larynx cancer depending of tissue location. There was a correlation of high incidence of positive cagA gene in larynx cancer tissue in supraglottic versus subglottic and glottic location. We observed a predominance of cagA gene in LSCC in patients with positive cervical lymph nodes and clinical stage T3 and T4.

CONCLUSIONS

H. pylori is present in larynx tissue and may be a possible carcinogen or co-carcinogen in LSCC development, but that must be addressed by future investigations. The presence of cagA gene in larynx cancer tissues significantly decreases survival rate and increases the disease recurrence possibilities.

Authors+Show Affiliations

Nicolaus Copernicus University of Toruń, Bydgoszcz, Poland. pburduk@wp.pl

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23860397

Citation

Burduk, Paweł Krzysztof. "Association Between Infection of Virulence cagA Gene Helicobacter Pylori and Laryngeal Squamous Cell Carcinoma." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 19, 2013, pp. 584-91.
Burduk PK. Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma. Med Sci Monit. 2013;19:584-91.
Burduk, P. K. (2013). Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 19, 584-91. https://doi.org/10.12659/MSM.889011
Burduk PK. Association Between Infection of Virulence cagA Gene Helicobacter Pylori and Laryngeal Squamous Cell Carcinoma. Med Sci Monit. 2013 Jul 17;19:584-91. PubMed PMID: 23860397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma. A1 - Burduk,Paweł Krzysztof, Y1 - 2013/07/17/ PY - 2013/7/18/entrez PY - 2013/7/19/pubmed PY - 2013/10/22/medline SP - 584 EP - 91 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med Sci Monit VL - 19 N2 - BACKGROUND: The aim of the study was to evaluate the presence of cagA gene Helicobacter pylori in etiopathogenesis of initiation and development of larynx squamous cell carcinoma (LSCC) and its predictable role as a prognostic factor. MATERIAL AND METHODS: The prospective, controlled study involved a series of 75 patients (65 male, 10 female, mean age 59.1 years, range 43 to 79 years) with larynx cancer. Samples of larynx cancerous tissue, each of 10-15 mg, were obtained from fresh tissues and were used for nucleic acid purification. DNA was extracted from 225 samples (larynx tumor - I (75), margin of tumor and normal tissue - II (75) and normal larynx tissue from opposite side to the tumor - III). All samples were subjected to H. pylori ureA detection by the PCR H. pylori diagnostic test. Samples that were positive for ureA H. pylori gene were evaluated for cagA H. pylori gene. RESULTS: Presence of H. pylori cagA gene was identified in 46,7% to 49,3% of 75 H. pylori ureA gene-positive larynx cancer depending of tissue location. There was a correlation of high incidence of positive cagA gene in larynx cancer tissue in supraglottic versus subglottic and glottic location. We observed a predominance of cagA gene in LSCC in patients with positive cervical lymph nodes and clinical stage T3 and T4. CONCLUSIONS: H. pylori is present in larynx tissue and may be a possible carcinogen or co-carcinogen in LSCC development, but that must be addressed by future investigations. The presence of cagA gene in larynx cancer tissues significantly decreases survival rate and increases the disease recurrence possibilities. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/23860397/Association_between_infection_of_virulence_cagA_gene_Helicobacter_pylori_and_laryngeal_squamous_cell_carcinoma_ DB - PRIME DP - Unbound Medicine ER -