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Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins.
PLoS One. 2013; 8(7):e69438.Plos

Abstract

The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.

Authors+Show Affiliations

Laboratorio de Inmunogenética, INIGEM-CONICET, Hospital de Clínicas José de San Martín, Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23861971

Citation

Ibañez, Andrés E., et al. "Unlipidated Outer Membrane Protein Omp16 (U-Omp16) From Brucella Spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins." PloS One, vol. 8, no. 7, 2013, pp. e69438.
Ibañez AE, Smaldini P, Coria LM, et al. Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins. PLoS ONE. 2013;8(7):e69438.
Ibañez, A. E., Smaldini, P., Coria, L. M., Delpino, M. V., Pacífico, L. G., Oliveira, S. C., Risso, G. S., Pasquevich, K. A., Fossati, C. A., Giambartolomei, G. H., Docena, G. H., & Cassataro, J. (2013). Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins. PloS One, 8(7), e69438. https://doi.org/10.1371/journal.pone.0069438
Ibañez AE, et al. Unlipidated Outer Membrane Protein Omp16 (U-Omp16) From Brucella Spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow's Milk Proteins. PLoS ONE. 2013;8(7):e69438. PubMed PMID: 23861971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unlipidated outer membrane protein Omp16 (U-Omp16) from Brucella spp. as nasal adjuvant induces a Th1 immune response and modulates the Th2 allergic response to cow's milk proteins. AU - Ibañez,Andrés E, AU - Smaldini,Paola, AU - Coria,Lorena M, AU - Delpino,María V, AU - Pacífico,Lucila G G, AU - Oliveira,Sergio C, AU - Risso,Gabriela S, AU - Pasquevich,Karina A, AU - Fossati,Carlos Alberto, AU - Giambartolomei,Guillermo H, AU - Docena,Guillermo H, AU - Cassataro,Juliana, Y1 - 2013/07/05/ PY - 2012/09/05/received PY - 2013/06/13/accepted PY - 2013/7/18/entrez PY - 2013/7/19/pubmed PY - 2014/2/14/medline SP - e69438 EP - e69438 JF - PloS one JO - PLoS ONE VL - 8 IS - 7 N2 - The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow's Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23861971/Unlipidated_outer_membrane_protein_Omp16__U_Omp16__from_Brucella_spp__as_nasal_adjuvant_induces_a_Th1_immune_response_and_modulates_the_Th2_allergic_response_to_cow's_milk_proteins_ L2 - http://dx.plos.org/10.1371/journal.pone.0069438 DB - PRIME DP - Unbound Medicine ER -