Tags

Type your tag names separated by a space and hit enter

Anti-inflammatory and antinociceptive activities of Homalium letestui.
Pharm Biol. 2013 Nov; 51(11):1459-66.PB

Abstract

CONTEXT

Homalium letestui Pellegr (Flacourtiaceae) is used in various decoctions traditionally by the Ibibios of the Niger Delta of Nigeria to treat stomach ulcer, malaria and other inflammatory diseases, as well as an aphrodisiac.

OBJECTIVE

To investigate the anti-inflammatory and antinociceptive activities of the stem extract of the plant.

MATERIALS AND METHODS

The ethanol stem extract (500, 750, 1000 mg/kg, i.p.) of H. letestui was investigated for anti-inflammatory activity using carrageenan, egg albumin-induced and xylene-induced ear edema models and analgesic activity using acetic acid-induced writhing, formalin-induced paw licking and thermal-induced pain models. The ethanol extract was administered to the animals orally, 30 min to 1 h depending on the model, before induction of inflammation/pain. The LD50 was also determined. GC-MS analysis of dichloromethane fraction was carried out.

RESULTS

The extract caused a significant (p < 0.05-0.001) reduction of inflammation induced by carrageenan (8.3-70.0%), egg albumin (10.0-71.42%) and xylene (39.39-84.84%). The extract also reduced significantly (p < 0.05-0.001) pain induced by acetic acid (44.22-73.65%), formalin (55.89-79.21%) and hot plate (93.0-214.5%). The LD50 was determined to be 4.38 ± 35.72 g/kg.

DISCUSSION AND CONCLUSION

The results of this study suggest that the ethanol stem extract of H. letestui possesses anti-inflammatory and analgesic properties which may in part be mediated through the chemical constituents of the plant as revealed by the GC-MS.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Okokon JE
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo , Uyo , Nigeria and.
Okokon PJ
No affiliation info available
Dar Farooq A
No affiliation info available
Choudhary MI
No affiliation info available

MeSH

Administration, OralAnalgesicsAnimalsAnti-Inflammatory AgentsBehavior, AnimalDisease Models, AnimalDose-Response Relationship, DrugEdemaEthanolFemaleGas Chromatography-Mass SpectrometryInflammationLethal Dose 50MaleMicePainPain ThresholdPhytotherapyPlant ExtractsPlant StemsPlants, MedicinalRatsRats, WistarSalicaceaeSolvents

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23862982

Citation

Okokon, Jude E., et al. "Anti-inflammatory and Antinociceptive Activities of Homalium Letestui." Pharmaceutical Biology, vol. 51, no. 11, 2013, pp. 1459-66.
Okokon JE, Okokon PJ, Dar Farooq A, et al. Anti-inflammatory and antinociceptive activities of Homalium letestui. Pharm Biol. 2013;51(11):1459-66.
Okokon, J. E., Okokon, P. J., Dar Farooq, A., & Choudhary, M. I. (2013). Anti-inflammatory and antinociceptive activities of Homalium letestui. Pharmaceutical Biology, 51(11), 1459-66. https://doi.org/10.3109/13880209.2013.799707
Okokon JE, et al. Anti-inflammatory and Antinociceptive Activities of Homalium Letestui. Pharm Biol. 2013;51(11):1459-66. PubMed PMID: 23862982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory and antinociceptive activities of Homalium letestui. AU - Okokon,Jude E, AU - Okokon,Patience J, AU - Dar Farooq,Ahsana, AU - Choudhary,Mohammed Iqbal, Y1 - 2013/07/18/ PY - 2013/7/19/entrez PY - 2013/7/19/pubmed PY - 2014/5/28/medline SP - 1459 EP - 66 JF - Pharmaceutical biology JO - Pharm Biol VL - 51 IS - 11 N2 - CONTEXT: Homalium letestui Pellegr (Flacourtiaceae) is used in various decoctions traditionally by the Ibibios of the Niger Delta of Nigeria to treat stomach ulcer, malaria and other inflammatory diseases, as well as an aphrodisiac. OBJECTIVE: To investigate the anti-inflammatory and antinociceptive activities of the stem extract of the plant. MATERIALS AND METHODS: The ethanol stem extract (500, 750, 1000 mg/kg, i.p.) of H. letestui was investigated for anti-inflammatory activity using carrageenan, egg albumin-induced and xylene-induced ear edema models and analgesic activity using acetic acid-induced writhing, formalin-induced paw licking and thermal-induced pain models. The ethanol extract was administered to the animals orally, 30 min to 1 h depending on the model, before induction of inflammation/pain. The LD50 was also determined. GC-MS analysis of dichloromethane fraction was carried out. RESULTS: The extract caused a significant (p < 0.05-0.001) reduction of inflammation induced by carrageenan (8.3-70.0%), egg albumin (10.0-71.42%) and xylene (39.39-84.84%). The extract also reduced significantly (p < 0.05-0.001) pain induced by acetic acid (44.22-73.65%), formalin (55.89-79.21%) and hot plate (93.0-214.5%). The LD50 was determined to be 4.38 ± 35.72 g/kg. DISCUSSION AND CONCLUSION: The results of this study suggest that the ethanol stem extract of H. letestui possesses anti-inflammatory and analgesic properties which may in part be mediated through the chemical constituents of the plant as revealed by the GC-MS. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/23862982/Anti_inflammatory_and_antinociceptive_activities_of_Homalium_letestui_ DB - PRIME DP - Unbound Medicine ER -