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Characterization of flavonoids and phenolic acids in Myrcia bella Cambess. using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS combined with NMR.
Molecules 2013; 18(7):8402-16M

Abstract

The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MS(n) method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts.

Authors+Show Affiliations

Botany Department, Institute of Biosciences, Univ. Estadual Paulista (UNESP), CEP 18618-970, Botucatu, Sao Paulo, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23863778

Citation

Saldanha, Luiz L., et al. "Characterization of Flavonoids and Phenolic Acids in Myrcia Bella Cambess. Using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS Combined With NMR." Molecules (Basel, Switzerland), vol. 18, no. 7, 2013, pp. 8402-16.
Saldanha LL, Vilegas W, Dokkedal AL. Characterization of flavonoids and phenolic acids in Myrcia bella Cambess. using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS combined with NMR. Molecules. 2013;18(7):8402-16.
Saldanha, L. L., Vilegas, W., & Dokkedal, A. L. (2013). Characterization of flavonoids and phenolic acids in Myrcia bella Cambess. using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS combined with NMR. Molecules (Basel, Switzerland), 18(7), pp. 8402-16. doi:10.3390/molecules18078402.
Saldanha LL, Vilegas W, Dokkedal AL. Characterization of Flavonoids and Phenolic Acids in Myrcia Bella Cambess. Using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS Combined With NMR. Molecules. 2013 Jul 16;18(7):8402-16. PubMed PMID: 23863778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of flavonoids and phenolic acids in Myrcia bella Cambess. using FIA-ESI-IT-MS(n) and HPLC-PAD-ESI-IT-MS combined with NMR. AU - Saldanha,Luiz L, AU - Vilegas,Wagner, AU - Dokkedal,Anne L, Y1 - 2013/07/16/ PY - 2013/06/07/received PY - 2013/07/05/revised PY - 2013/07/08/accepted PY - 2013/7/19/entrez PY - 2013/7/19/pubmed PY - 2014/2/11/medline SP - 8402 EP - 16 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 18 IS - 7 N2 - The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MS(n) method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/23863778/Characterization_of_flavonoids_and_phenolic_acids_in_Myrcia_bella_Cambess__using_FIA_ESI_IT_MS_n__and_HPLC_PAD_ESI_IT_MS_combined_with_NMR_ L2 - http://www.mdpi.com/resolver?pii=molecules18078402 DB - PRIME DP - Unbound Medicine ER -