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Lignans from Carthamus tinctorius suppress tryptophan breakdown via indoleamine 2,3-dioxygenase.
Phytomedicine. 2013 Oct 15; 20(13):1190-5.P

Abstract

Seed extracts of Carthamus tinctorius L. (Asteraceae), safflower, have been traditionally used to treat coronary disease, thrombotic disorders, and menstrual problems but also against cancer and depression. A possible effect of C. tinctorius compounds on tryptophan-degrading activity of enzyme indoleamine 2,3-dioxygenase (IDO) could explain many of its activities. To test for an effect of C. tinctorius extracts and isolated compounds on cytokine-induced IDO activity in immunocompetent cells in vitro methanol and ethylacetate seed extracts were prepared from cold pressed seed cakes of C. tinctorius and three lignan derivatives, trachelogenin, arctigenin and matairesinol were isolated. The influence on tryptophan breakdown was investigated in peripheral blood mononuclear cells (PBMCs). Effects were compared to neopterin production in the same cellular assay. Both seed extracts suppressed tryptophan breakdown in stimulated PBMC. The three structurally closely related isolates exerted differing suppressive activity on PBMC: arctigenin (IC50 26.5μM) and trachelogenin (IC50 of 57.4μM) showed higher activity than matairesinol (IC50 >200μM) to inhibit tryptophan breakdown. Effects on neopterin production were similar albeit generally less strong. Data show an immunosuppressive property of compounds which slows down IDO activity. The in vitro results support the view that some of the anti-inflammatory, anticancer and antidepressant properties of C. tinctorius lignans might relate to their suppressive influence on tryptophan breakdown.

Authors+Show Affiliations

University of Innsbruck, Institute of Pharmacy/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), 6020 Innsbruck, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23867649

Citation

Kuehnl, Susanne, et al. "Lignans From Carthamus Tinctorius Suppress Tryptophan Breakdown Via Indoleamine 2,3-dioxygenase." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 20, no. 13, 2013, pp. 1190-5.
Kuehnl S, Schroecksnadel S, Temml V, et al. Lignans from Carthamus tinctorius suppress tryptophan breakdown via indoleamine 2,3-dioxygenase. Phytomedicine. 2013;20(13):1190-5.
Kuehnl, S., Schroecksnadel, S., Temml, V., Gostner, J. M., Schennach, H., Schuster, D., Schwaiger, S., Rollinger, J. M., Fuchs, D., & Stuppner, H. (2013). Lignans from Carthamus tinctorius suppress tryptophan breakdown via indoleamine 2,3-dioxygenase. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 20(13), 1190-5. https://doi.org/10.1016/j.phymed.2013.06.006
Kuehnl S, et al. Lignans From Carthamus Tinctorius Suppress Tryptophan Breakdown Via Indoleamine 2,3-dioxygenase. Phytomedicine. 2013 Oct 15;20(13):1190-5. PubMed PMID: 23867649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lignans from Carthamus tinctorius suppress tryptophan breakdown via indoleamine 2,3-dioxygenase. AU - Kuehnl,Susanne, AU - Schroecksnadel,Sebastian, AU - Temml,Veronika, AU - Gostner,Johanna M, AU - Schennach,Harald, AU - Schuster,Daniela, AU - Schwaiger,Stefan, AU - Rollinger,Judith M, AU - Fuchs,Dietmar, AU - Stuppner,Hermann, Y1 - 2013/07/15/ PY - 2013/04/10/received PY - 2013/04/19/revised PY - 2013/06/01/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/8/26/medline KW - Asteraceae KW - Carthamus tinctorius KW - Indoleamine 2,3-Dioxygenase KW - Neopterin KW - Safflower SP - 1190 EP - 5 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 20 IS - 13 N2 - Seed extracts of Carthamus tinctorius L. (Asteraceae), safflower, have been traditionally used to treat coronary disease, thrombotic disorders, and menstrual problems but also against cancer and depression. A possible effect of C. tinctorius compounds on tryptophan-degrading activity of enzyme indoleamine 2,3-dioxygenase (IDO) could explain many of its activities. To test for an effect of C. tinctorius extracts and isolated compounds on cytokine-induced IDO activity in immunocompetent cells in vitro methanol and ethylacetate seed extracts were prepared from cold pressed seed cakes of C. tinctorius and three lignan derivatives, trachelogenin, arctigenin and matairesinol were isolated. The influence on tryptophan breakdown was investigated in peripheral blood mononuclear cells (PBMCs). Effects were compared to neopterin production in the same cellular assay. Both seed extracts suppressed tryptophan breakdown in stimulated PBMC. The three structurally closely related isolates exerted differing suppressive activity on PBMC: arctigenin (IC50 26.5μM) and trachelogenin (IC50 of 57.4μM) showed higher activity than matairesinol (IC50 >200μM) to inhibit tryptophan breakdown. Effects on neopterin production were similar albeit generally less strong. Data show an immunosuppressive property of compounds which slows down IDO activity. The in vitro results support the view that some of the anti-inflammatory, anticancer and antidepressant properties of C. tinctorius lignans might relate to their suppressive influence on tryptophan breakdown. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/23867649/Lignans_from_Carthamus_tinctorius_suppress_tryptophan_breakdown_via_indoleamine_23_dioxygenase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(13)00212-2 DB - PRIME DP - Unbound Medicine ER -