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Variation in the FADS1/2 gene cluster alters plasma n-6 PUFA and is weakly associated with hsCRP levels in healthy young adults.
Prostaglandins Leukot Essent Fatty Acids. 2013 Sep; 89(4):257-63.PL

Abstract

INTRODUCTION

Past research has reported that single nucleotide polymorphisms (SNPs) in fatty acid desaturase 1 and 2 (FADS1/2) can influence plasma fatty acid (FA) profiles. Changes in FA profiles are known to influence inflammatory processes; therefore both FA and SNPs in FADS1/2 may affect inflammation. The goals of this study were to (i) examine the relationships between individual n-6 FA and estimates of FA desaturation with circulating high sensitivity C-reactive protein (hsCRP) levels, and (ii) determine whether SNPs in FADS1/2 are associated with changes in hsCRP.

METHODS

FA and hsCRP were measured in fasted plasma samples from 878 healthy young adults (20-29yrs). Circulating levels of plasma linoleic (LA), γ-linolenic (GLA), dihomo-γ-linolenic (DGLA) and arachidonic (AA) acids were measured by gas chromatography and used to calculate desaturase indices for FADS1/2. Nineteen SNPs in FADS1/2 were genotyped in all subjects and six (rs174579, rs174593, rs174626, rs526126, rs968567 and rs17831757) were further analyzed.

RESULTS

Significant inverse associations were found between LA and hsCRP (p=8.55×10(-9)) and the FADS1 desaturase index and hsCRP (p=4.41×10(-6)). A significant positive association was found between DGLA and hsCRP (p=9.10×10(-11)). Several SNPs were associated with circulating levels of individual FA and desaturase indices, with minor allele carriers having lower AA levels and reduced desaturase indices. A single SNP in FADS2 (rs526126) was weakly associated with hsCRP (p=0.05).

CONCLUSIONS

This study highlights the relationships between FA and hsCRP, and confirms that FA are strongly influenced by SNPs in FADS1/2. Furthermore, we found weak evidence that SNPs in FADS1/2 may influence hsCRP levels in young adults.

Authors+Show Affiliations

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada N1G 2W1. Electronic address: kroke@uoguelph.ca.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23867726

Citation

Roke, Kaitlin, et al. "Variation in the FADS1/2 Gene Cluster Alters Plasma N-6 PUFA and Is Weakly Associated With hsCRP Levels in Healthy Young Adults." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 89, no. 4, 2013, pp. 257-63.
Roke K, Ralston JC, Abdelmagid S, et al. Variation in the FADS1/2 gene cluster alters plasma n-6 PUFA and is weakly associated with hsCRP levels in healthy young adults. Prostaglandins Leukot Essent Fatty Acids. 2013;89(4):257-63.
Roke, K., Ralston, J. C., Abdelmagid, S., Nielsen, D. E., Badawi, A., El-Sohemy, A., Ma, D. W., & Mutch, D. M. (2013). Variation in the FADS1/2 gene cluster alters plasma n-6 PUFA and is weakly associated with hsCRP levels in healthy young adults. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 89(4), 257-63. https://doi.org/10.1016/j.plefa.2013.06.003
Roke K, et al. Variation in the FADS1/2 Gene Cluster Alters Plasma N-6 PUFA and Is Weakly Associated With hsCRP Levels in Healthy Young Adults. Prostaglandins Leukot Essent Fatty Acids. 2013;89(4):257-63. PubMed PMID: 23867726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variation in the FADS1/2 gene cluster alters plasma n-6 PUFA and is weakly associated with hsCRP levels in healthy young adults. AU - Roke,Kaitlin, AU - Ralston,Jessica C, AU - Abdelmagid,Salma, AU - Nielsen,Daiva E, AU - Badawi,Alaa, AU - El-Sohemy,Ahmed, AU - Ma,David W L, AU - Mutch,David M, Y1 - 2013/07/16/ PY - 2013/02/18/received PY - 2013/06/04/revised PY - 2013/06/06/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/5/20/medline KW - AA KW - BMI KW - DGLA KW - Dihomo-γ-linolenic acid KW - FA KW - FADS1, FADS2 KW - FADS1/2 KW - FFQ KW - Fatty acid desaturase KW - GLA KW - High sensitivity C-reactive protein KW - Inflammation KW - LA KW - Linolenic acid KW - PUFA KW - SNPs KW - Single nucleotide polymorphism KW - TNH KW - Toronto Nutrigenomics and Health Study KW - arachidonic acid KW - body mass index KW - dihomo-γ-linolenic acid KW - fatty acid desaturase 1 and 2 KW - fatty acid desaturase gene cluster KW - fatty acids KW - food frequency questionnaire KW - high sensitivity C-reactive protein KW - hsCRP KW - linoleic acid KW - polyunsaturated fatty acids KW - single nucleotide polymorphisms KW - tSNPs KW - tag SNPs KW - γ-linolenic acid SP - 257 EP - 63 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 89 IS - 4 N2 - INTRODUCTION: Past research has reported that single nucleotide polymorphisms (SNPs) in fatty acid desaturase 1 and 2 (FADS1/2) can influence plasma fatty acid (FA) profiles. Changes in FA profiles are known to influence inflammatory processes; therefore both FA and SNPs in FADS1/2 may affect inflammation. The goals of this study were to (i) examine the relationships between individual n-6 FA and estimates of FA desaturation with circulating high sensitivity C-reactive protein (hsCRP) levels, and (ii) determine whether SNPs in FADS1/2 are associated with changes in hsCRP. METHODS: FA and hsCRP were measured in fasted plasma samples from 878 healthy young adults (20-29yrs). Circulating levels of plasma linoleic (LA), γ-linolenic (GLA), dihomo-γ-linolenic (DGLA) and arachidonic (AA) acids were measured by gas chromatography and used to calculate desaturase indices for FADS1/2. Nineteen SNPs in FADS1/2 were genotyped in all subjects and six (rs174579, rs174593, rs174626, rs526126, rs968567 and rs17831757) were further analyzed. RESULTS: Significant inverse associations were found between LA and hsCRP (p=8.55×10(-9)) and the FADS1 desaturase index and hsCRP (p=4.41×10(-6)). A significant positive association was found between DGLA and hsCRP (p=9.10×10(-11)). Several SNPs were associated with circulating levels of individual FA and desaturase indices, with minor allele carriers having lower AA levels and reduced desaturase indices. A single SNP in FADS2 (rs526126) was weakly associated with hsCRP (p=0.05). CONCLUSIONS: This study highlights the relationships between FA and hsCRP, and confirms that FA are strongly influenced by SNPs in FADS1/2. Furthermore, we found weak evidence that SNPs in FADS1/2 may influence hsCRP levels in young adults. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/23867726/Variation_in_the_FADS1/2_gene_cluster_alters_plasma_n_6_PUFA_and_is_weakly_associated_with_hsCRP_levels_in_healthy_young_adults_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(13)00132-4 DB - PRIME DP - Unbound Medicine ER -