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Effect of jujube honey on Candida albicans growth and biofilm formation.

Abstract

BACKGROUND AND AIMS

Candida species, especially Candida albicans, are major fungal pathogens of humans that are capable of causing superficial mucosal infections and systemic infections in humans. The aim of this study was to evaluate the jujube (Zizyphus spina-christi) honey for its in vitro inhibitory activity against pre-formed biofilm and its interference with the biofilm formation of C. albicans.

METHODS

The XTT reduction assay, scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to determine the inhibitory effect of Jujube honey on C. albicans biofilm. Changes in the infrared spectrum after treatment with honey were also determined by Fourier transform infrared (FTIR) spectroscopy.

RESULTS

Jujube honey affects biofilms by decreasing the size of mature biofilms and by disruption of their structure. At a concentration of 40% w/v, it interferes with formation of C. albicans biofilms and disrupts established biofilms. The SEM and AFM results indicated that this type of honey affected the cellular morphology of C. albicans and decreased biofilm thickness.

CONCLUSIONS

The present findings show that jujube honey has antifungal properties against C. albicans and has the ability to inhibit the formation of C. albicans biofilms and disrupt established biofilms.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Chair of Engineer Abdullah Ahmad Bugshan for Bee Research, Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.

    , , , , ,

    Source

    Archives of medical research 44:5 2013 Jul pg 352-60

    MeSH

    Analysis of Variance
    Antifungal Agents
    Biofilms
    Candida albicans
    Honey
    Humans
    Microbial Sensitivity Tests
    Microscopy, Electron, Scanning
    Phytotherapy
    Ziziphus

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23867789

    Citation

    TY - JOUR T1 - Effect of jujube honey on Candida albicans growth and biofilm formation. AU - Ansari,Mohammad Javed, AU - Al-Ghamdi,Ahmad, AU - Usmani,Salma, AU - Al-Waili,Noori S, AU - Sharma,Deepak, AU - Nuru,Adgaba, AU - Al-Attal,Yehya, Y1 - 2013/07/16/ PY - 2012/12/15/received PY - 2013/6/19/accepted PY - 2013/7/16/aheadofprint PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2013/12/18/medline KW - Atomic force microscopy KW - Biofilm KW - Candida albicans KW - Jujube honey KW - Scanning electron microscopy SP - 352 EP - 60 JF - Archives of medical research JO - Arch. Med. Res. VL - 44 IS - 5 N2 - BACKGROUND AND AIMS: Candida species, especially Candida albicans, are major fungal pathogens of humans that are capable of causing superficial mucosal infections and systemic infections in humans. The aim of this study was to evaluate the jujube (Zizyphus spina-christi) honey for its in vitro inhibitory activity against pre-formed biofilm and its interference with the biofilm formation of C. albicans. METHODS: The XTT reduction assay, scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to determine the inhibitory effect of Jujube honey on C. albicans biofilm. Changes in the infrared spectrum after treatment with honey were also determined by Fourier transform infrared (FTIR) spectroscopy. RESULTS: Jujube honey affects biofilms by decreasing the size of mature biofilms and by disruption of their structure. At a concentration of 40% w/v, it interferes with formation of C. albicans biofilms and disrupts established biofilms. The SEM and AFM results indicated that this type of honey affected the cellular morphology of C. albicans and decreased biofilm thickness. CONCLUSIONS: The present findings show that jujube honey has antifungal properties against C. albicans and has the ability to inhibit the formation of C. albicans biofilms and disrupt established biofilms. SN - 1873-5487 UR - https://www.unboundmedicine.com/medline/citation/23867789/Effect_of_jujube_honey_on_Candida_albicans_growth_and_biofilm_formation_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S0188-4409(13)00144-6 ER -