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The effect of nitrogen rate on transgenic corn Cry3Bb1 protein expression.
Pest Manag Sci. 2014 May; 70(5):763-70.PM

Abstract

BACKGROUND

Combining herbicide-resistant and Bacillus thuringiensis (Bt) traits in corn (Zea mays L.) hybrids may affect insect resistance management owing to volunteer corn. Some Bt toxins may be expressed at lower levels by nitrogen-deficient corn roots. Corn plants with sublethal levels of Bt expression could accelerate the evolution of Bt resistance in target insects. The present objective was to quantify the concentration of Bt (Cry3Bb1) in corn root tissue with varying tissue nitrogen concentrations.

RESULTS

Expression of Cry3Bb1 toxin in root tissue was highly variable, but there were no differences in the overall concentration of Cry3Bb1 expressed between roots taken from Cry3Bb1-positive volunteer and hybrid corn plants. The nitrogen rate did affect Cry3Bb1 expression in the greenhouse, less nitrogen resulted in decreased Cry3Bb1 expression, yet this result was not documented in the field.

CONCLUSION

A positive linear relationship of plant nitrogen status on Cry3Bb1 toxin expression was documented. Also, high variability in Cry3Bb1 expression is potentially problematic from an insect resistance management perspective. This variability could create a mosaic of toxin doses in the field, which does not fit into the high-dose refuge strategy and could alter predictions about the speed of evolution of resistance to Cry3Bb1 in western corn rootworm Diabrotica virgifera virgifera LeConte.

Authors+Show Affiliations

Purdue University Department of Botany and Plant Pathology, West Lafayette, IN, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23868342

Citation

Marquardt, Paul T., et al. "The Effect of Nitrogen Rate On Transgenic Corn Cry3Bb1 Protein Expression." Pest Management Science, vol. 70, no. 5, 2014, pp. 763-70.
Marquardt PT, Krupke CH, Camberato JJ, et al. The effect of nitrogen rate on transgenic corn Cry3Bb1 protein expression. Pest Manag Sci. 2014;70(5):763-70.
Marquardt, P. T., Krupke, C. H., Camberato, J. J., & Johnson, W. G. (2014). The effect of nitrogen rate on transgenic corn Cry3Bb1 protein expression. Pest Management Science, 70(5), 763-70. https://doi.org/10.1002/ps.3611
Marquardt PT, et al. The Effect of Nitrogen Rate On Transgenic Corn Cry3Bb1 Protein Expression. Pest Manag Sci. 2014;70(5):763-70. PubMed PMID: 23868342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of nitrogen rate on transgenic corn Cry3Bb1 protein expression. AU - Marquardt,Paul T, AU - Krupke,Christian H, AU - Camberato,James J, AU - Johnson,William G, Y1 - 2013/08/27/ PY - 2013/01/08/received PY - 2013/07/11/revised PY - 2013/07/18/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/12/19/medline KW - Bt KW - Cry3Bb1 KW - insect resistance management KW - nitrogen KW - volunteer corn KW - western corn rootworm SP - 763 EP - 70 JF - Pest management science JO - Pest Manag Sci VL - 70 IS - 5 N2 - BACKGROUND: Combining herbicide-resistant and Bacillus thuringiensis (Bt) traits in corn (Zea mays L.) hybrids may affect insect resistance management owing to volunteer corn. Some Bt toxins may be expressed at lower levels by nitrogen-deficient corn roots. Corn plants with sublethal levels of Bt expression could accelerate the evolution of Bt resistance in target insects. The present objective was to quantify the concentration of Bt (Cry3Bb1) in corn root tissue with varying tissue nitrogen concentrations. RESULTS: Expression of Cry3Bb1 toxin in root tissue was highly variable, but there were no differences in the overall concentration of Cry3Bb1 expressed between roots taken from Cry3Bb1-positive volunteer and hybrid corn plants. The nitrogen rate did affect Cry3Bb1 expression in the greenhouse, less nitrogen resulted in decreased Cry3Bb1 expression, yet this result was not documented in the field. CONCLUSION: A positive linear relationship of plant nitrogen status on Cry3Bb1 toxin expression was documented. Also, high variability in Cry3Bb1 expression is potentially problematic from an insect resistance management perspective. This variability could create a mosaic of toxin doses in the field, which does not fit into the high-dose refuge strategy and could alter predictions about the speed of evolution of resistance to Cry3Bb1 in western corn rootworm Diabrotica virgifera virgifera LeConte. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/23868342/The_effect_of_nitrogen_rate_on_transgenic_corn_Cry3Bb1_protein_expression_ L2 - https://doi.org/10.1002/ps.3611 DB - PRIME DP - Unbound Medicine ER -