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Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats.
J Physiol Biochem. 2014 Mar; 70(1):1-14.JP

Abstract

Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Aseervatham GS
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India.
Sivasudha T
No affiliation info available
Sasikumar JM
No affiliation info available
Christabel PH
No affiliation info available
Jeyadevi R
No affiliation info available
Ananth DA
No affiliation info available

MeSH

AcetaminophenAnalgesics, Non-NarcoticAnimalsBenzothiazolesBiphenyl CompoundsCatalaseChemical and Drug Induced Liver InjuryChromatography, Thin LayerCytoprotectionFree Radical ScavengersFruitHepatocytesLiverMaleNitric OxideOxidative StressPicratesPlant ExtractsPolyphenolsPouteriaRatsRats, WistarSulfonic AcidsSuperoxide Dismutase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23872880

Citation

Aseervatham, G Smilin Bell, et al. "Antioxidant and Hepatoprotective Potential of Pouteria Campechiana On Acetaminophen-induced Hepatic Toxicity in Rats." Journal of Physiology and Biochemistry, vol. 70, no. 1, 2014, pp. 1-14.
Aseervatham GS, Sivasudha T, Sasikumar JM, et al. Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. J Physiol Biochem. 2014;70(1):1-14.
Aseervatham, G. S., Sivasudha, T., Sasikumar, J. M., Christabel, P. H., Jeyadevi, R., & Ananth, D. A. (2014). Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. Journal of Physiology and Biochemistry, 70(1), 1-14. https://doi.org/10.1007/s13105-013-0274-3
Aseervatham GS, et al. Antioxidant and Hepatoprotective Potential of Pouteria Campechiana On Acetaminophen-induced Hepatic Toxicity in Rats. J Physiol Biochem. 2014;70(1):1-14. PubMed PMID: 23872880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. AU - Aseervatham,G Smilin Bell, AU - Sivasudha,T, AU - Sasikumar,J M, AU - Christabel,P Hephzibah, AU - Jeyadevi,R, AU - Ananth,D Arul, Y1 - 2013/07/20/ PY - 2013/02/25/received PY - 2013/07/02/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/11/5/medline SP - 1 EP - 14 JF - Journal of physiology and biochemistry JO - J Physiol Biochem VL - 70 IS - 1 N2 - Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity. SN - 1877-8755 UR - https://www.unboundmedicine.com/medline/citation/23872880/Antioxidant_and_hepatoprotective_potential_of_Pouteria_campechiana_on_acetaminophen_induced_hepatic_toxicity_in_rats_ L2 - https://dx.doi.org/10.1007/s13105-013-0274-3 DB - PRIME DP - Unbound Medicine ER -