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Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats.

Abstract

Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity.

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  • Authors+Show Affiliations

    ,

    Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India.

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    Source

    MeSH

    Acetaminophen
    Analgesics, Non-Narcotic
    Animals
    Benzothiazoles
    Biphenyl Compounds
    Catalase
    Chemical and Drug Induced Liver Injury
    Chromatography, Thin Layer
    Cytoprotection
    Free Radical Scavengers
    Fruit
    Hepatocytes
    Liver
    Male
    Nitric Oxide
    Oxidative Stress
    Picrates
    Plant Extracts
    Polyphenols
    Pouteria
    Rats
    Rats, Wistar
    Sulfonic Acids
    Superoxide Dismutase

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    23872880

    Citation

    Aseervatham, G Smilin Bell, et al. "Antioxidant and Hepatoprotective Potential of Pouteria Campechiana On Acetaminophen-induced Hepatic Toxicity in Rats." Journal of Physiology and Biochemistry, vol. 70, no. 1, 2014, pp. 1-14.
    Aseervatham GS, Sivasudha T, Sasikumar JM, et al. Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. J Physiol Biochem. 2014;70(1):1-14.
    Aseervatham, G. S., Sivasudha, T., Sasikumar, J. M., Christabel, P. H., Jeyadevi, R., & Ananth, D. A. (2014). Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. Journal of Physiology and Biochemistry, 70(1), pp. 1-14. doi:10.1007/s13105-013-0274-3.
    Aseervatham GS, et al. Antioxidant and Hepatoprotective Potential of Pouteria Campechiana On Acetaminophen-induced Hepatic Toxicity in Rats. J Physiol Biochem. 2014;70(1):1-14. PubMed PMID: 23872880.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Antioxidant and hepatoprotective potential of Pouteria campechiana on acetaminophen-induced hepatic toxicity in rats. AU - Aseervatham,G Smilin Bell, AU - Sivasudha,T, AU - Sasikumar,J M, AU - Christabel,P Hephzibah, AU - Jeyadevi,R, AU - Ananth,D Arul, Y1 - 2013/07/20/ PY - 2013/02/25/received PY - 2013/07/02/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/11/5/medline SP - 1 EP - 14 JF - Journal of physiology and biochemistry JO - J. Physiol. Biochem. VL - 70 IS - 1 N2 - Pouteria campechiana (Kunth) Baehni. is used as a remedy for coronary trouble, liver disorders, epilepsy, skin disease, and ulcer. Therefore, the present study aims to investigate the antioxidant and hepatoprotective effect of polyphenolic-rich P. campechiana fruit extract against acetaminophen-intoxicated rats. Total phenolic and flavonoid contents of egg fruit were estimated followed by the determination of antioxidant activities. Treatment with P. campechiana fruit extract effectively scavenged the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed by high-performance thin-layer chromatography (HPTLC). The animals were treated with acetaminophen (250 mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). Acetaminophen treatment was found to trigger an oxidative stress in liver, leading to an increase of serum marker enzymes. However, treatment with P. campechiana fruit extract significantly reduced the elevated liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase) and glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen-induced hepatotoxicity. SN - 1877-8755 UR - https://www.unboundmedicine.com/medline/citation/23872880/Antioxidant_and_hepatoprotective_potential_of_Pouteria_campechiana_on_acetaminophen_induced_hepatic_toxicity_in_rats_ L2 - https://dx.doi.org/10.1007/s13105-013-0274-3 DB - PRIME DP - Unbound Medicine ER -