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Carnosine and taurine treatments decreased oxidative stress and tissue damage induced by D-galactose in rat liver.
J Physiol Biochem 2014; 70(1):15-25JP

Abstract

D-galactose (GAL) causes aging-related changes and oxidative stress in the organism. We investigated the effect of carnosine (CAR) or taurine (TAU), having antioxidant effects, on hepatic injury and oxidative stress in GAL-treated rats. Rats received GAL (300 mg/kg; s.c.; 5 days/week) alone or together with CAR (250 mg/kg/daily; i.p.; 5 days/week) or TAU (2.5 % w/w; in rat chow) for 2 months. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-0050x), and glutathione transferase (GST) activities were determined. Hepatic expressions of B cell lymphoma-2 (Bcl-2), Bax and Ki-67 were evaluated. Serum ALT, AST, hepatic MDA, and PC levels were observed to increase in GAL-treated rats. Hepatic Bax expression, but not Bcl-2, increased, Ki-67 expression decreased. GAL treatment caused decreases in GSH levels, SOD and GSH-Px activities in the liver. Hepatic mRNA expressions of SOD, but not GSH-Px, also diminished. CAR or TAU treatments caused significant decreases in serum ALT and AST activities. These treatments decreased apoptosis and increased proliferation and ameliorated histopathological findings in the livers of GAL-treated rats. Both CAR and TAU reduced MDA and PC levels and elevated GSH levels, SOD and GSH-Px (non significant in TAU + GAL group) activities. These treatments did not alter hepatic mRNA expressions of SOD and GSH-Px enzymes. Our results indicate that CAR and TAU restored liver prooxidant status together with histopathological amelioration in GAL-induced liver damage.

Authors+Show Affiliations

Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23872881

Citation

Kalaz, Esra Betül, et al. "Carnosine and Taurine Treatments Decreased Oxidative Stress and Tissue Damage Induced By D-galactose in Rat Liver." Journal of Physiology and Biochemistry, vol. 70, no. 1, 2014, pp. 15-25.
Kalaz EB, Çoban J, Aydın AF, et al. Carnosine and taurine treatments decreased oxidative stress and tissue damage induced by D-galactose in rat liver. J Physiol Biochem. 2014;70(1):15-25.
Kalaz, E. B., Çoban, J., Aydın, A. F., Doğan-Ekici, I., Doğru-Abbasoğlu, S., Öztezcan, S., & Uysal, M. (2014). Carnosine and taurine treatments decreased oxidative stress and tissue damage induced by D-galactose in rat liver. Journal of Physiology and Biochemistry, 70(1), pp. 15-25. doi:10.1007/s13105-013-0275-2.
Kalaz EB, et al. Carnosine and Taurine Treatments Decreased Oxidative Stress and Tissue Damage Induced By D-galactose in Rat Liver. J Physiol Biochem. 2014;70(1):15-25. PubMed PMID: 23872881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carnosine and taurine treatments decreased oxidative stress and tissue damage induced by D-galactose in rat liver. AU - Kalaz,Esra Betül, AU - Çoban,Jale, AU - Aydın,A Fatih, AU - Doğan-Ekici,Işın, AU - Doğru-Abbasoğlu,Semra, AU - Öztezcan,Serdar, AU - Uysal,Müjdat, Y1 - 2013/07/20/ PY - 2013/05/02/received PY - 2013/07/02/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/11/5/medline SP - 15 EP - 25 JF - Journal of physiology and biochemistry JO - J. Physiol. Biochem. VL - 70 IS - 1 N2 - D-galactose (GAL) causes aging-related changes and oxidative stress in the organism. We investigated the effect of carnosine (CAR) or taurine (TAU), having antioxidant effects, on hepatic injury and oxidative stress in GAL-treated rats. Rats received GAL (300 mg/kg; s.c.; 5 days/week) alone or together with CAR (250 mg/kg/daily; i.p.; 5 days/week) or TAU (2.5 % w/w; in rat chow) for 2 months. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-0050x), and glutathione transferase (GST) activities were determined. Hepatic expressions of B cell lymphoma-2 (Bcl-2), Bax and Ki-67 were evaluated. Serum ALT, AST, hepatic MDA, and PC levels were observed to increase in GAL-treated rats. Hepatic Bax expression, but not Bcl-2, increased, Ki-67 expression decreased. GAL treatment caused decreases in GSH levels, SOD and GSH-Px activities in the liver. Hepatic mRNA expressions of SOD, but not GSH-Px, also diminished. CAR or TAU treatments caused significant decreases in serum ALT and AST activities. These treatments decreased apoptosis and increased proliferation and ameliorated histopathological findings in the livers of GAL-treated rats. Both CAR and TAU reduced MDA and PC levels and elevated GSH levels, SOD and GSH-Px (non significant in TAU + GAL group) activities. These treatments did not alter hepatic mRNA expressions of SOD and GSH-Px enzymes. Our results indicate that CAR and TAU restored liver prooxidant status together with histopathological amelioration in GAL-induced liver damage. SN - 1877-8755 UR - https://www.unboundmedicine.com/medline/citation/23872881/Carnosine_and_taurine_treatments_decreased_oxidative_stress_and_tissue_damage_induced_by_D_galactose_in_rat_liver_ L2 - https://dx.doi.org/10.1007/s13105-013-0275-2 DB - PRIME DP - Unbound Medicine ER -