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Involvement of amygdalar protein kinase A, but not calcium/calmodulin-dependent protein kinase II, in the reconsolidation of cocaine-related contextual memories in rats.
Psychopharmacology (Berl). 2014 Jan; 231(1):55-65.P

Abstract

RATIONALE

Contextual control over drug relapse depends on the successful reconsolidation and retention of context-response-cocaine associations in long-term memory stores. The basolateral amygdala (BLA) plays a critical role in cocaine memory reconsolidation and subsequent drug context-induced cocaine-seeking behavior; however, less is known about the cellular mechanisms of this phenomenon.

OBJECTIVES

The present study evaluated the hypothesis that protein kinase A (PKA) and calcium/calmodulin-dependent protein kinase II (CaMKII) activation in the BLA is necessary for the reconsolidation of context-response-cocaine memories that promote subsequent drug context-induced cocaine-seeking behavior.

METHODS

Rats were trained to lever-press for cocaine infusions in a distinct context, followed by extinction training in a different context. Rats were then briefly re-exposed to the previously cocaine-paired context or an unpaired context in order to reactivate cocaine-related contextual memories and initiate their reconsolidation or to provide a similar behavioral experience without explicit cocaine-related memory reactivation, respectively. Immediately after this session, rats received bilateral microinfusions of vehicle, the PKA inhibitor, Rp-adenosine 3',5'-cyclic monophosphorothioate triethylammonium salt (Rp-cAMPS), or the CaMKII inhibitor, KN-93, into the BLA or the posterior caudate putamen (anatomical control region). Rats were then tested for cocaine-seeking behavior (responses on the previously cocaine-paired lever) in the cocaine-paired context and the extinction context.

RESULTS

Intra-BLA infusion of Rp-cAMPS, but not KN-93, following cocaine memory reconsolidation impaired subsequent cocaine-seeking behavior in a dose-dependent, site-specific, and memory reactivation-dependent fashion.

CONCLUSIONS

PKA, but not CaMKII, activation in the BLA is critical for cocaine memory re-stabilization processes that facilitate subsequent drug context-induced instrumental cocaine-seeking behavior.

Authors+Show Affiliations

Department of Psychology, University of North Carolina, CB# 3270, Davie Hall, Chapel Hill, NC, 27599-3270, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23873418

Citation

Arguello, Amy A., et al. "Involvement of Amygdalar Protein Kinase A, but Not Calcium/calmodulin-dependent Protein Kinase II, in the Reconsolidation of Cocaine-related Contextual Memories in Rats." Psychopharmacology, vol. 231, no. 1, 2014, pp. 55-65.
Arguello AA, Hodges MA, Wells AM, et al. Involvement of amygdalar protein kinase A, but not calcium/calmodulin-dependent protein kinase II, in the reconsolidation of cocaine-related contextual memories in rats. Psychopharmacology (Berl). 2014;231(1):55-65.
Arguello, A. A., Hodges, M. A., Wells, A. M., Lara, H., Xie, X., & Fuchs, R. A. (2014). Involvement of amygdalar protein kinase A, but not calcium/calmodulin-dependent protein kinase II, in the reconsolidation of cocaine-related contextual memories in rats. Psychopharmacology, 231(1), 55-65. https://doi.org/10.1007/s00213-013-3203-9
Arguello AA, et al. Involvement of Amygdalar Protein Kinase A, but Not Calcium/calmodulin-dependent Protein Kinase II, in the Reconsolidation of Cocaine-related Contextual Memories in Rats. Psychopharmacology (Berl). 2014;231(1):55-65. PubMed PMID: 23873418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of amygdalar protein kinase A, but not calcium/calmodulin-dependent protein kinase II, in the reconsolidation of cocaine-related contextual memories in rats. AU - Arguello,Amy A, AU - Hodges,Matthew A, AU - Wells,Audrey M, AU - Lara,Honorio,3rd AU - Xie,Xiaohu, AU - Fuchs,Rita A, Y1 - 2013/07/20/ PY - 2013/02/11/received PY - 2013/07/03/accepted PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2014/8/19/medline SP - 55 EP - 65 JF - Psychopharmacology JO - Psychopharmacology (Berl) VL - 231 IS - 1 N2 - RATIONALE: Contextual control over drug relapse depends on the successful reconsolidation and retention of context-response-cocaine associations in long-term memory stores. The basolateral amygdala (BLA) plays a critical role in cocaine memory reconsolidation and subsequent drug context-induced cocaine-seeking behavior; however, less is known about the cellular mechanisms of this phenomenon. OBJECTIVES: The present study evaluated the hypothesis that protein kinase A (PKA) and calcium/calmodulin-dependent protein kinase II (CaMKII) activation in the BLA is necessary for the reconsolidation of context-response-cocaine memories that promote subsequent drug context-induced cocaine-seeking behavior. METHODS: Rats were trained to lever-press for cocaine infusions in a distinct context, followed by extinction training in a different context. Rats were then briefly re-exposed to the previously cocaine-paired context or an unpaired context in order to reactivate cocaine-related contextual memories and initiate their reconsolidation or to provide a similar behavioral experience without explicit cocaine-related memory reactivation, respectively. Immediately after this session, rats received bilateral microinfusions of vehicle, the PKA inhibitor, Rp-adenosine 3',5'-cyclic monophosphorothioate triethylammonium salt (Rp-cAMPS), or the CaMKII inhibitor, KN-93, into the BLA or the posterior caudate putamen (anatomical control region). Rats were then tested for cocaine-seeking behavior (responses on the previously cocaine-paired lever) in the cocaine-paired context and the extinction context. RESULTS: Intra-BLA infusion of Rp-cAMPS, but not KN-93, following cocaine memory reconsolidation impaired subsequent cocaine-seeking behavior in a dose-dependent, site-specific, and memory reactivation-dependent fashion. CONCLUSIONS: PKA, but not CaMKII, activation in the BLA is critical for cocaine memory re-stabilization processes that facilitate subsequent drug context-induced instrumental cocaine-seeking behavior. SN - 1432-2072 UR - https://www.unboundmedicine.com/medline/citation/23873418/Involvement_of_amygdalar_protein_kinase_A_but_not_calcium/calmodulin_dependent_protein_kinase_II_in_the_reconsolidation_of_cocaine_related_contextual_memories_in_rats_ L2 - https://dx.doi.org/10.1007/s00213-013-3203-9 DB - PRIME DP - Unbound Medicine ER -