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RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC.
J Bacteriol. 2013 Sep; 195(18):4274-82.JB

Abstract

Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class 1 gene product FlhD(4)C(2). The flhDC promoter is a target for a broad range of transcriptional regulators that bind within the flhDC promoter region and either negatively or positively regulate flhDC operon transcription. In this work, we demonstrate that the RflM protein is a key component of flhDC regulation. Transposon mutagenesis was performed to investigate a previously described autoinhibitory effect of the flagellar master regulatory complex FlhD(4)C(2). RflM is a LuxR homolog that functions as a flagellar class 1 transcriptional repressor. RflM was found to be the negative regulator of flhDC expression that is responsible for the formerly described autoinhibitory effect of the FlhD(4)C(2) complex on flhDC operon transcription (K. Kutsukake, Mol. Gen. Genet. 254:440-448, 1997). We conclude that upon commencement of flagellar gene expression, the FlhD(4)C(2) complex initiates a regulatory feedback loop by activating rflM gene expression. rflM encodes a transcriptional repressor, RflM, which fine-tunes flhDC expression levels.

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Authors+Show Affiliations

Singer HM
Microbiologie, Département de Médecine, Université de Fribourg, Fribourg, Switzerland.
Erhardt M
No affiliation info available
Hughes KT
No affiliation info available

MeSH

Bacterial ProteinsDNA Transposable ElementsDown-RegulationFlagellaGene Expression Regulation, BacterialGenes, RegulatorMutagenesis, InsertionalOperonPromoter Regions, GeneticSalmonella typhimuriumTrans-Activators

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23873910

Citation

Singer, Hanna M., et al. "RflM Functions as a Transcriptional Repressor in the Autogenous Control of the Salmonella Flagellar Master Operon FlhDC." Journal of Bacteriology, vol. 195, no. 18, 2013, pp. 4274-82.
Singer HM, Erhardt M, Hughes KT. RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC. J Bacteriol. 2013;195(18):4274-82.
Singer, H. M., Erhardt, M., & Hughes, K. T. (2013). RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC. Journal of Bacteriology, 195(18), 4274-82. https://doi.org/10.1128/JB.00728-13
Singer HM, Erhardt M, Hughes KT. RflM Functions as a Transcriptional Repressor in the Autogenous Control of the Salmonella Flagellar Master Operon FlhDC. J Bacteriol. 2013;195(18):4274-82. PubMed PMID: 23873910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC. AU - Singer,Hanna M, AU - Erhardt,Marc, AU - Hughes,Kelly T, Y1 - 2013/07/19/ PY - 2013/7/23/entrez PY - 2013/7/23/pubmed PY - 2013/11/6/medline SP - 4274 EP - 82 JF - Journal of bacteriology JO - J Bacteriol VL - 195 IS - 18 N2 - Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class 1 gene product FlhD(4)C(2). The flhDC promoter is a target for a broad range of transcriptional regulators that bind within the flhDC promoter region and either negatively or positively regulate flhDC operon transcription. In this work, we demonstrate that the RflM protein is a key component of flhDC regulation. Transposon mutagenesis was performed to investigate a previously described autoinhibitory effect of the flagellar master regulatory complex FlhD(4)C(2). RflM is a LuxR homolog that functions as a flagellar class 1 transcriptional repressor. RflM was found to be the negative regulator of flhDC expression that is responsible for the formerly described autoinhibitory effect of the FlhD(4)C(2) complex on flhDC operon transcription (K. Kutsukake, Mol. Gen. Genet. 254:440-448, 1997). We conclude that upon commencement of flagellar gene expression, the FlhD(4)C(2) complex initiates a regulatory feedback loop by activating rflM gene expression. rflM encodes a transcriptional repressor, RflM, which fine-tunes flhDC expression levels. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/23873910/RflM_functions_as_a_transcriptional_repressor_in_the_autogenous_control_of_the_Salmonella_Flagellar_master_operon_flhDC_ L2 - https://journals.asm.org/doi/10.1128/JB.00728-13?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -