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Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions.
Biomaterials. 2013 Oct; 34(32):7884-94.B

Abstract

Silver nanoparticles (AgNPs) have anti-cancer effect. However, whether and how these particles could inhibit the growth of acute myeloid leukemia (AML) cells is unclear. In the present study, we prepared AgNPs with various sizes and investigated their cytotoxic effect on AML cells. We found that AgNPs could inhibit the viability of AML cells including the isolates from AML patients. AgNPs caused the production of reactive oxygen species (ROS), losses of mitochondrial membrane potential (MMP), DNA damage and apoptosis. Both vitamin C (Vit C) and N-acetyl-L-cysteine (NAC) could completely reverse the generation of ROS upon AgNPs, however only NAC but not Vit C could protect the cells from losses of MMP, DNA damage and apoptosis thoroughly. Similar results were obtained when cells were treated with silver ions alone. As NAC was not only an antioxidant to scavenge ROS but also a silver ion chelator, these data supported the model that both generation of ROS and release of silver ions played critical roles in the AgNPs-induced cytotoxic effect against AML cells. Taken together, this work elucidated the cytotoxic effect of AgNPs on AML cells and their underlying mechanism and might have significant impact on AML treatment.

Authors+Show Affiliations

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23876760

Citation

Guo, Dawei, et al. "Anti-leukemia Activity of PVP-coated Silver Nanoparticles Via Generation of Reactive Oxygen Species and Release of Silver Ions." Biomaterials, vol. 34, no. 32, 2013, pp. 7884-94.
Guo D, Zhu L, Huang Z, et al. Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions. Biomaterials. 2013;34(32):7884-94.
Guo, D., Zhu, L., Huang, Z., Zhou, H., Ge, Y., Ma, W., Wu, J., Zhang, X., Zhou, X., Zhang, Y., Zhao, Y., & Gu, N. (2013). Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions. Biomaterials, 34(32), 7884-94. https://doi.org/10.1016/j.biomaterials.2013.07.015
Guo D, et al. Anti-leukemia Activity of PVP-coated Silver Nanoparticles Via Generation of Reactive Oxygen Species and Release of Silver Ions. Biomaterials. 2013;34(32):7884-94. PubMed PMID: 23876760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions. AU - Guo,Dawei, AU - Zhu,Lingying, AU - Huang,Zhihai, AU - Zhou,Haixia, AU - Ge,Yue, AU - Ma,Wenjuan, AU - Wu,Jie, AU - Zhang,Xiuyan, AU - Zhou,Xuefeng, AU - Zhang,Yu, AU - Zhao,Yun, AU - Gu,Ning, Y1 - 2013/07/19/ PY - 2013/05/10/received PY - 2013/07/03/accepted PY - 2013/7/24/entrez PY - 2013/7/24/pubmed PY - 2014/3/1/medline KW - Acute myeloid leukemia KW - Cytotoxicity KW - Reactive oxygen species KW - Silver ions KW - Silver nanoparticles SP - 7884 EP - 94 JF - Biomaterials JO - Biomaterials VL - 34 IS - 32 N2 - Silver nanoparticles (AgNPs) have anti-cancer effect. However, whether and how these particles could inhibit the growth of acute myeloid leukemia (AML) cells is unclear. In the present study, we prepared AgNPs with various sizes and investigated their cytotoxic effect on AML cells. We found that AgNPs could inhibit the viability of AML cells including the isolates from AML patients. AgNPs caused the production of reactive oxygen species (ROS), losses of mitochondrial membrane potential (MMP), DNA damage and apoptosis. Both vitamin C (Vit C) and N-acetyl-L-cysteine (NAC) could completely reverse the generation of ROS upon AgNPs, however only NAC but not Vit C could protect the cells from losses of MMP, DNA damage and apoptosis thoroughly. Similar results were obtained when cells were treated with silver ions alone. As NAC was not only an antioxidant to scavenge ROS but also a silver ion chelator, these data supported the model that both generation of ROS and release of silver ions played critical roles in the AgNPs-induced cytotoxic effect against AML cells. Taken together, this work elucidated the cytotoxic effect of AgNPs on AML cells and their underlying mechanism and might have significant impact on AML treatment. SN - 1878-5905 UR - https://www.unboundmedicine.com/medline/citation/23876760/Anti_leukemia_activity_of_PVP_coated_silver_nanoparticles_via_generation_of_reactive_oxygen_species_and_release_of_silver_ions_ DB - PRIME DP - Unbound Medicine ER -