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Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite.
Analyst. 2013 Sep 21; 138(18):5365-70.A

Abstract

A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine-graphene (AuNPs/Th-G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine viaπ-stacking interaction, and then AuNPs were electrodeposited onto the Th-G surface. The morphology and conductivity of the AuNPs/Th-G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th-G nanocomposite to prepare an electrochemical biosensing platform. The aptamer-thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).

Authors+Show Affiliations

College of Sciences, Shanghai University, Shanghai 200444, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23877321

Citation

Zhang, Zhao, et al. "Aptamer-linked Biosensor for Thrombin Based On AuNPs/thionine-graphene Nanocomposite." The Analyst, vol. 138, no. 18, 2013, pp. 5365-70.
Zhang Z, Luo L, Zhu L, et al. Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite. Analyst. 2013;138(18):5365-70.
Zhang, Z., Luo, L., Zhu, L., Ding, Y., Deng, D., & Wang, Z. (2013). Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite. The Analyst, 138(18), 5365-70. https://doi.org/10.1039/c3an01006f
Zhang Z, et al. Aptamer-linked Biosensor for Thrombin Based On AuNPs/thionine-graphene Nanocomposite. Analyst. 2013 Sep 21;138(18):5365-70. PubMed PMID: 23877321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite. AU - Zhang,Zhao, AU - Luo,Liqiang, AU - Zhu,Limei, AU - Ding,Yaping, AU - Deng,Dongmei, AU - Wang,Zhenxin, Y1 - 2013/07/23/ PY - 2013/7/24/entrez PY - 2013/7/24/pubmed PY - 2014/2/4/medline SP - 5365 EP - 70 JF - The Analyst JO - Analyst VL - 138 IS - 18 N2 - A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine-graphene (AuNPs/Th-G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine viaπ-stacking interaction, and then AuNPs were electrodeposited onto the Th-G surface. The morphology and conductivity of the AuNPs/Th-G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th-G nanocomposite to prepare an electrochemical biosensing platform. The aptamer-thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3). SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/23877321/Aptamer_linked_biosensor_for_thrombin_based_on_AuNPs/thionine_graphene_nanocomposite_ L2 - https://doi.org/10.1039/c3an01006f DB - PRIME DP - Unbound Medicine ER -