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Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae.
New Phytol. 2013 Nov; 200(3):808-819.NP

Abstract

Bacterial plant-pathogenic Xanthomonas strains translocate transcription activator-like (TAL) effectors into plant cells to function as specific transcription factors. Only a few plant target genes of TAL effectors have been identified, so far. Three plant SWEET genes encoding putative sugar transporters are known to be induced by TAL effectors from rice-pathogenic Xanthomonas oryzae pv. oryzae (Xoo). We predict and validate that expression of OsSWEET14 is induced by a novel TAL effector, Tal5, from an African Xoo strain. Artificial TAL effectors (ArtTALs) were constructed to individually target 20 SWEET orthologs in rice. They were used as designer virulence factors to study which rice SWEET genes can support Xoo virulence. The Tal5 target box differs from those of the already known TAL effectors TalC, AvrXa7 and PthXo3, which also induce expression of OsSWEET14, suggesting evolutionary convergence on key targets. ArtTALs efficiently complemented an Xoo talC mutant, demonstrating that specific induction of OsSWEET14 is the key target of TalC. ArtTALs that specifically target individual members of the rice SWEET family revealed three known and two novel SWEET genes to support bacterial virulence. Our results demonstrate that five phylogenetically close SWEET proteins, which presumably act as sucrose transporters, can support Xoo virulence.

Authors+Show Affiliations

Department of Genetics, Martin Luther University Halle-Wittenberg, Weinbergweg 10, D-06120, Halle (Saale), Germany.UMR 186 IRD-UM2-Cirad 'Résistance des Plantes aux Bioagresseurs', BP 64501, 34394, Montpellier Cedex 5, France. Earth and Life Institute, Applied Microbiology Phytopathology, Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium.UMR 186 IRD-UM2-Cirad 'Résistance des Plantes aux Bioagresseurs', BP 64501, 34394, Montpellier Cedex 5, France.UMR 186 IRD-UM2-Cirad 'Résistance des Plantes aux Bioagresseurs', BP 64501, 34394, Montpellier Cedex 5, France.Department of Genetics, Martin Luther University Halle-Wittenberg, Weinbergweg 10, D-06120, Halle (Saale), Germany.UMR 186 IRD-UM2-Cirad 'Résistance des Plantes aux Bioagresseurs', BP 64501, 34394, Montpellier Cedex 5, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23879865

Citation

Streubel, Jana, et al. "Five Phylogenetically Close Rice SWEET Genes Confer TAL Effector-mediated Susceptibility to Xanthomonas Oryzae Pv. Oryzae." The New Phytologist, vol. 200, no. 3, 2013, pp. 808-819.
Streubel J, Pesce C, Hutin M, et al. Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae. New Phytol. 2013;200(3):808-819.
Streubel, J., Pesce, C., Hutin, M., Koebnik, R., Boch, J., & Szurek, B. (2013). Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae. The New Phytologist, 200(3), 808-819. https://doi.org/10.1111/nph.12411
Streubel J, et al. Five Phylogenetically Close Rice SWEET Genes Confer TAL Effector-mediated Susceptibility to Xanthomonas Oryzae Pv. Oryzae. New Phytol. 2013;200(3):808-819. PubMed PMID: 23879865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae. AU - Streubel,Jana, AU - Pesce,Céline, AU - Hutin,Mathilde, AU - Koebnik,Ralf, AU - Boch,Jens, AU - Szurek,Boris, Y1 - 2013/07/24/ PY - 2013/04/17/received PY - 2013/06/03/accepted PY - 2013/7/25/entrez PY - 2013/7/25/pubmed PY - 2014/4/30/medline KW - OsSWEET KW - TALE KW - designer TALE KW - plant pathogen KW - plant resistance KW - rice KW - type-III effector KW - virulence SP - 808 EP - 819 JF - The New phytologist JO - New Phytol VL - 200 IS - 3 N2 - Bacterial plant-pathogenic Xanthomonas strains translocate transcription activator-like (TAL) effectors into plant cells to function as specific transcription factors. Only a few plant target genes of TAL effectors have been identified, so far. Three plant SWEET genes encoding putative sugar transporters are known to be induced by TAL effectors from rice-pathogenic Xanthomonas oryzae pv. oryzae (Xoo). We predict and validate that expression of OsSWEET14 is induced by a novel TAL effector, Tal5, from an African Xoo strain. Artificial TAL effectors (ArtTALs) were constructed to individually target 20 SWEET orthologs in rice. They were used as designer virulence factors to study which rice SWEET genes can support Xoo virulence. The Tal5 target box differs from those of the already known TAL effectors TalC, AvrXa7 and PthXo3, which also induce expression of OsSWEET14, suggesting evolutionary convergence on key targets. ArtTALs efficiently complemented an Xoo talC mutant, demonstrating that specific induction of OsSWEET14 is the key target of TalC. ArtTALs that specifically target individual members of the rice SWEET family revealed three known and two novel SWEET genes to support bacterial virulence. Our results demonstrate that five phylogenetically close SWEET proteins, which presumably act as sucrose transporters, can support Xoo virulence. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/23879865/Five_phylogenetically_close_rice_SWEET_genes_confer_TAL_effector_mediated_susceptibility_to_Xanthomonas_oryzae_pv__oryzae_ L2 - https://doi.org/10.1111/nph.12411 DB - PRIME DP - Unbound Medicine ER -