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Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage.
Planta Med. 2013 Sep; 79(14):1319-23.PM

Abstract

Paeonia suffruticosa, an important traditional herbal medicine, has been reported to prevent the pathogenesis of diabetic nephropathy through modulating advanced glycation end products-induced inflammatory and oxidative stress responses. However, little was known about the protective effect of the two major compounds in P. suffruticosa, paeoniflorin and oxypaeoniflora, on advanced glycation end products-induced mesangial cell damage. In the present study, we investigated the protective activities of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced oxidative stress and inflammation in mesangial cells HBZY-1. The IC50 values of paeoniflorin and oxypaeoniflora for inhibiting 2,2'-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were 4.197 × 10-4 M and 1.002 × 10-4 M, respectively. The pretreatment with paeoniflorin and oxypaeoniflora (10-8-10-4 M) significantly increased advanced glycation end product-induced glutathione peroxidase and catalase activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin and oxypaeoniflora could inhibit remarkably the migration of macrophages. Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced glycation end products-induced inflammation cytokines interleukin-6 and monocyte chemoattractant protein-1 levels in ELISA and western blot analysis in a dose-dependent manner. Taken together, our data provided the reliable evidence that paeniflorin and oxypaeniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells. Paeniflorin and oxypaeniflora might therefore have a beneficial effect in the treatment of diabetic nephropathy.

Authors+Show Affiliations

Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23881455

Citation

Zhang, Ming-hua, et al. "Antioxidative and Anti-inflammatory Activities of Paeoniflorin and Oxypaeoniflora On AGEs-induced Mesangial Cell Damage." Planta Medica, vol. 79, no. 14, 2013, pp. 1319-23.
Zhang MH, Feng L, Zhu MM, et al. Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage. Planta Med. 2013;79(14):1319-23.
Zhang, M. H., Feng, L., Zhu, M. M., Gu, J. F., Wu, C., & Jia, X. B. (2013). Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage. Planta Medica, 79(14), 1319-23. https://doi.org/10.1055/s-0033-1350649
Zhang MH, et al. Antioxidative and Anti-inflammatory Activities of Paeoniflorin and Oxypaeoniflora On AGEs-induced Mesangial Cell Damage. Planta Med. 2013;79(14):1319-23. PubMed PMID: 23881455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative and anti-inflammatory activities of paeoniflorin and oxypaeoniflora on AGEs-induced mesangial cell damage. AU - Zhang,Ming-hua, AU - Feng,Liang, AU - Zhu,Mao-mao, AU - Gu,Jun-fei, AU - Wu,Chan, AU - Jia,Xiao-bin, Y1 - 2013/07/23/ PY - 2013/7/25/entrez PY - 2013/7/25/pubmed PY - 2014/4/9/medline SP - 1319 EP - 23 JF - Planta medica JO - Planta Med VL - 79 IS - 14 N2 - Paeonia suffruticosa, an important traditional herbal medicine, has been reported to prevent the pathogenesis of diabetic nephropathy through modulating advanced glycation end products-induced inflammatory and oxidative stress responses. However, little was known about the protective effect of the two major compounds in P. suffruticosa, paeoniflorin and oxypaeoniflora, on advanced glycation end products-induced mesangial cell damage. In the present study, we investigated the protective activities of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced oxidative stress and inflammation in mesangial cells HBZY-1. The IC50 values of paeoniflorin and oxypaeoniflora for inhibiting 2,2'-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were 4.197 × 10-4 M and 1.002 × 10-4 M, respectively. The pretreatment with paeoniflorin and oxypaeoniflora (10-8-10-4 M) significantly increased advanced glycation end product-induced glutathione peroxidase and catalase activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin and oxypaeoniflora could inhibit remarkably the migration of macrophages. Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced glycation end products-induced inflammation cytokines interleukin-6 and monocyte chemoattractant protein-1 levels in ELISA and western blot analysis in a dose-dependent manner. Taken together, our data provided the reliable evidence that paeniflorin and oxypaeniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells. Paeniflorin and oxypaeniflora might therefore have a beneficial effect in the treatment of diabetic nephropathy. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/23881455/Antioxidative_and_anti_inflammatory_activities_of_paeoniflorin_and_oxypaeoniflora_on_AGEs_induced_mesangial_cell_damage_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0033-1350649 DB - PRIME DP - Unbound Medicine ER -