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Assessment of genotoxic, cytotoxic, and protective effects of Salacia crassifolia (Mart. Ex. Schult.) G. Don. stem bark fractions in mice.
Genet Mol Res. 2013 Jul 03; 12(3):2167-77.GM

Abstract

Salacia crassifolia (Mart. Ex. Schult.) G. Don., popularly known in Brazil as "bacupari", "cascudo", and "saputá", is a shrub of the Celastraceae family that is unique to the Brazilian Cerrado region. In folk medicine, this plant has been mainly used to treat skin cancer and gastric ulcers. In the present study, the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of S. crassifolia stem bark fractions (hexane, ethyl acetate, and hydroalcoholic extracts) were evaluated using the mouse bone marrow micronucleus test. Our results showed that none of the S. crassifolia fractions led to a significant increase in the frequency of micronucleated polychromatic erythrocytes (MNPCE) (P > 0.05), suggesting the absence of genotoxicity. In the antigenotoxicity assessment, a significant decrease in the MNPCE frequency was observed in all fractions of this plant (P < 0.05), demonstrating its protective action against genotoxicity induced by mitomycin C (MMC), which was used as the positive control. Only the hexane fraction of S. crassifolia significantly decreased the poly- and normochromatic erythrocyte ratio (PCE/NCE) in all doses tested (P < 0.05), demonstrating its cytotoxic activity. In association with MMC, both ethyl acetate and hydroalcoholic fractions significantly increased the PCE/NCE ratio in almost all doses tested (P < 0.05), demonstrating the protective action of S. crassifolia against the cytotoxic effect of the positive control. In contrast, the hexane fraction presented a significant decrease in the PCE/NCE ratio in all treatments (P < 0.05), demonstrating an increase in this plant's cytotoxicity in mouse bone marrow cells.

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Authors+Show Affiliations

Carneiro CC
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
Silva CR
No affiliation info available
Menezes AC
No affiliation info available
Pérez CN
No affiliation info available
Chen-Chen L
No affiliation info available

MeSH

AnimalsAntibiotics, AntineoplasticBone Marrow CellsChemical FractionationCytotoxinsDNA DamageErythrocytesMiceMicronuclei, Chromosome-DefectiveMitomycinPlant BarkPlant ExtractsPlant StemsSalacia

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23884760

Citation

Carneiro, C C., et al. "Assessment of Genotoxic, Cytotoxic, and Protective Effects of Salacia Crassifolia (Mart. Ex. Schult.) G. Don. Stem Bark Fractions in Mice." Genetics and Molecular Research : GMR, vol. 12, no. 3, 2013, pp. 2167-77.
Carneiro CC, Silva CR, Menezes AC, et al. Assessment of genotoxic, cytotoxic, and protective effects of Salacia crassifolia (Mart. Ex. Schult.) G. Don. stem bark fractions in mice. Genet Mol Res. 2013;12(3):2167-77.
Carneiro, C. C., Silva, C. R., Menezes, A. C., Pérez, C. N., & Chen-Chen, L. (2013). Assessment of genotoxic, cytotoxic, and protective effects of Salacia crassifolia (Mart. Ex. Schult.) G. Don. stem bark fractions in mice. Genetics and Molecular Research : GMR, 12(3), 2167-77. https://doi.org/10.4238/2013.July.3.1
Carneiro CC, et al. Assessment of Genotoxic, Cytotoxic, and Protective Effects of Salacia Crassifolia (Mart. Ex. Schult.) G. Don. Stem Bark Fractions in Mice. Genet Mol Res. 2013 Jul 3;12(3):2167-77. PubMed PMID: 23884760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of genotoxic, cytotoxic, and protective effects of Salacia crassifolia (Mart. Ex. Schult.) G. Don. stem bark fractions in mice. AU - Carneiro,C C, AU - Silva,C R, AU - Menezes,A C S, AU - Pérez,C N, AU - Chen-Chen,L, Y1 - 2013/07/03/ PY - 2013/7/26/entrez PY - 2013/7/26/pubmed PY - 2014/2/13/medline SP - 2167 EP - 77 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 12 IS - 3 N2 - Salacia crassifolia (Mart. Ex. Schult.) G. Don., popularly known in Brazil as "bacupari", "cascudo", and "saputá", is a shrub of the Celastraceae family that is unique to the Brazilian Cerrado region. In folk medicine, this plant has been mainly used to treat skin cancer and gastric ulcers. In the present study, the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of S. crassifolia stem bark fractions (hexane, ethyl acetate, and hydroalcoholic extracts) were evaluated using the mouse bone marrow micronucleus test. Our results showed that none of the S. crassifolia fractions led to a significant increase in the frequency of micronucleated polychromatic erythrocytes (MNPCE) (P > 0.05), suggesting the absence of genotoxicity. In the antigenotoxicity assessment, a significant decrease in the MNPCE frequency was observed in all fractions of this plant (P < 0.05), demonstrating its protective action against genotoxicity induced by mitomycin C (MMC), which was used as the positive control. Only the hexane fraction of S. crassifolia significantly decreased the poly- and normochromatic erythrocyte ratio (PCE/NCE) in all doses tested (P < 0.05), demonstrating its cytotoxic activity. In association with MMC, both ethyl acetate and hydroalcoholic fractions significantly increased the PCE/NCE ratio in almost all doses tested (P < 0.05), demonstrating the protective action of S. crassifolia against the cytotoxic effect of the positive control. In contrast, the hexane fraction presented a significant decrease in the PCE/NCE ratio in all treatments (P < 0.05), demonstrating an increase in this plant's cytotoxicity in mouse bone marrow cells. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/23884760/Assessment_of_genotoxic_cytotoxic_and_protective_effects_of_Salacia_crassifolia__Mart__Ex__Schult___G__Don__stem_bark_fractions_in_mice_ L2 - http://www.geneticsmr.com/articles/2312 DB - PRIME DP - Unbound Medicine ER -