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Characterization of Dicrocoelium dendriticum isolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal DNA.
J Helminthol. 2015 Jan; 89(1):124-9.JH

Abstract

The genetic variations in internal transcribed spacers (ITS) spanning ITS-1, 5.8S and ITS-2 rDNA of Dicrocoelium dendriticum, isolated from sheep and goats in four geographical regions in Shaanxi province, were examined. The lengths of ITS-1, 5.8S and ITS-2 rDNA sequences for D. dendriticum were 749 bp, 161 bp and 234 bp, respectively. Intra-specific sequence variations of D. dendriticum were 0-0.5% for ITS-1 and 0-1.3% for ITS-2 rDNA, while the inter-specific variations among species in genus Dicrocoelium in ITS-2 rDNA were 3.4-12.3%. Phylogenetic analysis based on sequences of ITS-2 rDNA showed that all D. dendriticum isolates in the present study were grouped with reference D. dendriticum isolates from sheep and goats, and D. dendriticum isolates from cattle and Japanese serow were clustered in a sister clade. However, the phylogenetic tree could not reveal geographically genetic relationships of D. dendriticum isolates in different origins and hosts. These findings provided basic information for further study of molecular epidemiology and control of D. dendriticum infection in Shaanxi province as well as in the world.

Authors+Show Affiliations

College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.College of Veterinary Medicine, Northwest A&F University,Yangling,Shaanxi Province712100,PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23890204

Citation

Bian, Q Q., et al. "Characterization of Dicrocoelium Dendriticum Isolates From Small Ruminants in Shaanxi Province, North-western China, Using Internal Transcribed Spacers of Nuclear Ribosomal DNA." Journal of Helminthology, vol. 89, no. 1, 2015, pp. 124-9.
Bian QQ, Zhao GH, Jia YQ, et al. Characterization of Dicrocoelium dendriticum isolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal DNA. J Helminthol. 2015;89(1):124-9.
Bian, Q. Q., Zhao, G. H., Jia, Y. Q., Fang, Y. Q., Cheng, W. Y., Du, S. Z., Ma, X. T., & Lin, Q. (2015). Characterization of Dicrocoelium dendriticum isolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal DNA. Journal of Helminthology, 89(1), 124-9. https://doi.org/10.1017/S0022149X13000503
Bian QQ, et al. Characterization of Dicrocoelium Dendriticum Isolates From Small Ruminants in Shaanxi Province, North-western China, Using Internal Transcribed Spacers of Nuclear Ribosomal DNA. J Helminthol. 2015;89(1):124-9. PubMed PMID: 23890204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Dicrocoelium dendriticum isolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal DNA. AU - Bian,Q Q, AU - Zhao,G H, AU - Jia,Y Q, AU - Fang,Y Q, AU - Cheng,W Y, AU - Du,S Z, AU - Ma,X T, AU - Lin,Q, Y1 - 2013/07/26/ PY - 2013/7/30/entrez PY - 2013/7/31/pubmed PY - 2016/2/3/medline SP - 124 EP - 9 JF - Journal of helminthology JO - J. Helminthol. VL - 89 IS - 1 N2 - The genetic variations in internal transcribed spacers (ITS) spanning ITS-1, 5.8S and ITS-2 rDNA of Dicrocoelium dendriticum, isolated from sheep and goats in four geographical regions in Shaanxi province, were examined. The lengths of ITS-1, 5.8S and ITS-2 rDNA sequences for D. dendriticum were 749 bp, 161 bp and 234 bp, respectively. Intra-specific sequence variations of D. dendriticum were 0-0.5% for ITS-1 and 0-1.3% for ITS-2 rDNA, while the inter-specific variations among species in genus Dicrocoelium in ITS-2 rDNA were 3.4-12.3%. Phylogenetic analysis based on sequences of ITS-2 rDNA showed that all D. dendriticum isolates in the present study were grouped with reference D. dendriticum isolates from sheep and goats, and D. dendriticum isolates from cattle and Japanese serow were clustered in a sister clade. However, the phylogenetic tree could not reveal geographically genetic relationships of D. dendriticum isolates in different origins and hosts. These findings provided basic information for further study of molecular epidemiology and control of D. dendriticum infection in Shaanxi province as well as in the world. SN - 1475-2697 UR - https://www.unboundmedicine.com/medline/citation/23890204/Characterization_of_Dicrocoelium_dendriticum_isolates_from_small_ruminants_in_Shaanxi_Province_north_western_China_using_internal_transcribed_spacers_of_nuclear_ribosomal_DNA_ L2 - https://www.cambridge.org/core/product/identifier/S0022149X13000503/type/journal_article DB - PRIME DP - Unbound Medicine ER -