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Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal specimen.
Diagn Microbiol Infect Dis. 2013 Oct; 77(2):101-5.DM

Abstract

A prospective, multicenter trial was designed to compare the performance characteristics of the cobas® 4800 (Roche Diagnostics, Indianapolis, IN, USA) and m2000 real-time™ (Abbott Molecular Inc., Des Plaines, IL, USA) assays for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in rectal and self-collected vaginal swabs. Rectal (n = 234) or self-collected vaginal swabs (n = 687) were obtained from consenting individuals visiting their general practitioners, dermatologists, gynecologists, sexually transmitted disease clinics, or family planning centers from May 2010 to February 2011. High concordance rates (≥96%) were observed between the cobas® 4800 and m2000 real-time™ assays for CT/NG detection in both rectal and self-collected vaginal swabs. The performance profiles confirm the usefulness of both kinds of swab types for CT and NG detection using described nucleic acid amplification tests assays. Based on this study, rectal and self-collected vaginal swabs offer a noninvasive alternative, which may improve screening for CT and NG infections.

Authors+Show Affiliations

Department of Medical Microbiology, University Medical Center Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23891224

Citation

Geelen, Tanja H., et al. "Performance of Cobas® 4800 and M2000 Real-time™ Assays for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Rectal and Self-collected Vaginal Specimen." Diagnostic Microbiology and Infectious Disease, vol. 77, no. 2, 2013, pp. 101-5.
Geelen TH, Rossen JW, Beerens AM, et al. Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal specimen. Diagn Microbiol Infect Dis. 2013;77(2):101-5.
Geelen, T. H., Rossen, J. W., Beerens, A. M., Poort, L., Morré, S. A., Ritmeester, W. S., van Kruchten, H. E., van de Pas, M. M., & Savelkoul, P. H. (2013). Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal specimen. Diagnostic Microbiology and Infectious Disease, 77(2), 101-5. https://doi.org/10.1016/j.diagmicrobio.2013.06.020
Geelen TH, et al. Performance of Cobas® 4800 and M2000 Real-time™ Assays for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Rectal and Self-collected Vaginal Specimen. Diagn Microbiol Infect Dis. 2013;77(2):101-5. PubMed PMID: 23891224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of cobas® 4800 and m2000 real-time™ assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and self-collected vaginal specimen. AU - Geelen,Tanja H, AU - Rossen,John W, AU - Beerens,Antoine M, AU - Poort,Linda, AU - Morré,Servaas A, AU - Ritmeester,Wilma S, AU - van Kruchten,Harry E, AU - van de Pas,Masja M, AU - Savelkoul,Paul H M, Y1 - 2013/07/23/ PY - 2013/03/08/received PY - 2013/06/10/revised PY - 2013/06/14/accepted PY - 2013/7/30/entrez PY - 2013/7/31/pubmed PY - 2014/7/1/medline KW - Chlamydia trachomatis KW - Diagnostic screening KW - Neisseria gonorrhoeae KW - PCR KW - Rectal swabs KW - Self-collected vaginal swabs KW - Sexually transmitted infections SP - 101 EP - 5 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 77 IS - 2 N2 - A prospective, multicenter trial was designed to compare the performance characteristics of the cobas® 4800 (Roche Diagnostics, Indianapolis, IN, USA) and m2000 real-time™ (Abbott Molecular Inc., Des Plaines, IL, USA) assays for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in rectal and self-collected vaginal swabs. Rectal (n = 234) or self-collected vaginal swabs (n = 687) were obtained from consenting individuals visiting their general practitioners, dermatologists, gynecologists, sexually transmitted disease clinics, or family planning centers from May 2010 to February 2011. High concordance rates (≥96%) were observed between the cobas® 4800 and m2000 real-time™ assays for CT/NG detection in both rectal and self-collected vaginal swabs. The performance profiles confirm the usefulness of both kinds of swab types for CT and NG detection using described nucleic acid amplification tests assays. Based on this study, rectal and self-collected vaginal swabs offer a noninvasive alternative, which may improve screening for CT and NG infections. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/23891224/Performance_of_cobas®_4800_and_m2000_real_time™_assays_for_detection_of_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_in_rectal_and_self_collected_vaginal_specimen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(13)00375-1 DB - PRIME DP - Unbound Medicine ER -