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Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers.
Int J Med Microbiol. 2013 Dec; 303(8):475-83.IJ

Abstract

Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. To date, little information is available on the molecular basis of antimicrobial resistance in APEC in Africa. Therefore, the objective of this study was to characterize the virulence and antimicrobial resistance of multidrug-resistant APEC isolated from septicemic broilers in Egypt at the molecular level. Among 91 non-repetitive E. coli isolates, 73 (80.2%) carried three or more of the APEC virulence genes iroN, ompT, iss, iutA, and hlyF. All 73 APEC isolates showed multidrug resistance phenotypes, particularly against ampicillin, tetracycline, spectinomycin, streptomycin, kanamycin, and trimethoprim/sulfamethoxazole. PCR and DNA sequencing identified class 1 and class 2 integrons in 34 (46.6%) and seven (9.6%) isolates, respectively. The β-lactamase-encoding genes, bla(TEM-1), bla(TEM-104), bla(CMY-2), bla(OXA-30), bla(CTX-M-15), and bla(SHV-2); tetracycline resistance genes, tet(A), tet(B), tet(C), tet(D), and tet(E); the plasmid-mediated quinolone resistance genes, qnrA1, qnrB2, qnrS1, and aac(6')-Ib-cr, and florfenicol resistance gene, floR, were also identified in 69 (94.5%), 67 (91.8%), 47 (64.4%), and 13 (17.8%) isolates, respectively. To the best of our knowledge, this is the first report of molecular characterization of antimicrobial resistance in APEC strains from Africa.

Authors+Show Affiliations

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23891276

Citation

Ahmed, Ashraf M., et al. "Molecular Characterization of Multidrug-resistant Avian Pathogenic Escherichia Coli Isolated From Septicemic Broilers." International Journal of Medical Microbiology : IJMM, vol. 303, no. 8, 2013, pp. 475-83.
Ahmed AM, Shimamoto T, Shimamoto T. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Int J Med Microbiol. 2013;303(8):475-83.
Ahmed, A. M., Shimamoto, T., & Shimamoto, T. (2013). Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. International Journal of Medical Microbiology : IJMM, 303(8), 475-83. https://doi.org/10.1016/j.ijmm.2013.06.009
Ahmed AM, Shimamoto T, Shimamoto T. Molecular Characterization of Multidrug-resistant Avian Pathogenic Escherichia Coli Isolated From Septicemic Broilers. Int J Med Microbiol. 2013;303(8):475-83. PubMed PMID: 23891276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. AU - Ahmed,Ashraf M, AU - Shimamoto,Toshi, AU - Shimamoto,Tadashi, Y1 - 2013/06/20/ PY - 2013/02/05/received PY - 2013/06/08/revised PY - 2013/06/16/accepted PY - 2013/7/30/entrez PY - 2013/7/31/pubmed PY - 2014/6/24/medline KW - APEC KW - Integrons KW - Septicemic broilers KW - Virulence KW - β-Lactamase SP - 475 EP - 83 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 303 IS - 8 N2 - Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. To date, little information is available on the molecular basis of antimicrobial resistance in APEC in Africa. Therefore, the objective of this study was to characterize the virulence and antimicrobial resistance of multidrug-resistant APEC isolated from septicemic broilers in Egypt at the molecular level. Among 91 non-repetitive E. coli isolates, 73 (80.2%) carried three or more of the APEC virulence genes iroN, ompT, iss, iutA, and hlyF. All 73 APEC isolates showed multidrug resistance phenotypes, particularly against ampicillin, tetracycline, spectinomycin, streptomycin, kanamycin, and trimethoprim/sulfamethoxazole. PCR and DNA sequencing identified class 1 and class 2 integrons in 34 (46.6%) and seven (9.6%) isolates, respectively. The β-lactamase-encoding genes, bla(TEM-1), bla(TEM-104), bla(CMY-2), bla(OXA-30), bla(CTX-M-15), and bla(SHV-2); tetracycline resistance genes, tet(A), tet(B), tet(C), tet(D), and tet(E); the plasmid-mediated quinolone resistance genes, qnrA1, qnrB2, qnrS1, and aac(6')-Ib-cr, and florfenicol resistance gene, floR, were also identified in 69 (94.5%), 67 (91.8%), 47 (64.4%), and 13 (17.8%) isolates, respectively. To the best of our knowledge, this is the first report of molecular characterization of antimicrobial resistance in APEC strains from Africa. SN - 1618-0607 UR - https://www.unboundmedicine.com/medline/citation/23891276/Molecular_characterization_of_multidrug_resistant_avian_pathogenic_Escherichia_coli_isolated_from_septicemic_broilers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(13)00088-X DB - PRIME DP - Unbound Medicine ER -