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Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites.
Biosens Bioelectron. 2013 Dec 15; 50:356-61.BB

Abstract

In this work, a sandwich-format electrochemical immunosensor for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was fabricated using biofunctional carboxyl graphene nanosheets (CGS) as immunosensing probes, which were fabricated by means of immobilization of toluidine blue (TB) and labeled anti-CEA (Ab2,1), Prussian blue (PB) and anti-AFP (Ab2,2) successively on CGS. The capture anti-CEA (Ab1,1) and anti-AFP (Ab1,2) were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) modified electrode through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide (EDC/NHS). Experimental results revealed that this sandwich-type immunoassay enabled simultaneous detection of CEA and AFP with linear range of 0.5-60 ng mL(-1) for both analytes. The detection limit was 0.1 ng mL(-1) for CEA and 0.05 ng mL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from the standard ELISA method. And the negligible cross-reactivity between the two analytes allows it to possess potential promise in clinical diagnosis.

Authors+Show Affiliations

Department of Chemistry, Capital Normal University, Beijing 100048, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23891798

Citation

Chen, Xia, et al. "Electrochemical Immunosensor for Simultaneous Detection of Multiplex Cancer Biomarkers Based On Graphene Nanocomposites." Biosensors & Bioelectronics, vol. 50, 2013, pp. 356-61.
Chen X, Jia X, Han J, et al. Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites. Biosens Bioelectron. 2013;50:356-61.
Chen, X., Jia, X., Han, J., Ma, J., & Ma, Z. (2013). Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites. Biosensors & Bioelectronics, 50, 356-61. https://doi.org/10.1016/j.bios.2013.06.054
Chen X, et al. Electrochemical Immunosensor for Simultaneous Detection of Multiplex Cancer Biomarkers Based On Graphene Nanocomposites. Biosens Bioelectron. 2013 Dec 15;50:356-61. PubMed PMID: 23891798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites. AU - Chen,Xia, AU - Jia,Xinle, AU - Han,Jingman, AU - Ma,Jie, AU - Ma,Zhanfang, Y1 - 2013/07/04/ PY - 2013/04/14/received PY - 2013/06/01/revised PY - 2013/06/26/accepted PY - 2013/7/30/entrez PY - 2013/7/31/pubmed PY - 2014/3/29/medline KW - Alpha-fetoprotein KW - Carboxyl graphene nanosheet-Prussian blue KW - Carboxyl graphene nanosheet-toluidine blue KW - Carcinoembryonic antigen KW - Chitosan-Au nanoparticles SP - 356 EP - 61 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 50 N2 - In this work, a sandwich-format electrochemical immunosensor for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was fabricated using biofunctional carboxyl graphene nanosheets (CGS) as immunosensing probes, which were fabricated by means of immobilization of toluidine blue (TB) and labeled anti-CEA (Ab2,1), Prussian blue (PB) and anti-AFP (Ab2,2) successively on CGS. The capture anti-CEA (Ab1,1) and anti-AFP (Ab1,2) were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) modified electrode through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide (EDC/NHS). Experimental results revealed that this sandwich-type immunoassay enabled simultaneous detection of CEA and AFP with linear range of 0.5-60 ng mL(-1) for both analytes. The detection limit was 0.1 ng mL(-1) for CEA and 0.05 ng mL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from the standard ELISA method. And the negligible cross-reactivity between the two analytes allows it to possess potential promise in clinical diagnosis. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23891798/Electrochemical_immunosensor_for_simultaneous_detection_of_multiplex_cancer_biomarkers_based_on_graphene_nanocomposites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00451-X DB - PRIME DP - Unbound Medicine ER -